Abstract
Copper is an integral part of a number of proteins and thus an essential trace metal. However, free copper ions can be highly toxic and every organism has to carefully control its bioavailability. Eukaryotes contain three copper chaperones; Atx1p/Atox1 which delivers copper to ATP7 transporters located in the trans-Golgi network, Cox17 which provides copper to the mitochondrial cytochrome c oxidase, and CCS which is a copper chaperone for superoxide dismutase 1. Here we describe the knockout phenotype of the Drosophila homolog of mammalian Atox1 (ATX1 in yeast). Atox1−/− flies develop normally, though at reduced numbers, and the eclosing flies are fertile. However, the mutants are unable to develop on low-copper food. Furthermore, the intestinal copper importer Ctr1B, which is regulated by copper demand, fails to be induced upon copper starvation in Atox1−/− larvae. At the same time, intestinal metallothionein is upregulated. This phenotype, which resembles the one of the ATP7 mutant, is best explained by intestinal copper accumulation, combined with insufficient delivery to the rest of the body. In addition, compared to controls, Drosophila Atox1 mutants are relatively insensitive to the anticancer drug cisplatin, a compound which is also imported via Ctr1 copper transporters and was recently found to bind mammalian Atox1.
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Acknowledgments
We thank Drs. Johannes Bischof and Konrad Basler (University of Zürich) for the Phi C31 integration system, attP fly lines and the flies carrying transposase (y w; + ; ∆2-3 Sb/TM2). We are also grateful to Till Strassen for the maintenance of fly stocks, to Dr. Dominik Steiger for valuable discussions and to Drs. George Hausmann (University of Zürich) and Dennis J. Thiele (Duke University, NC) for critical reading of the manuscript. This work was supported by the Kanton Zürich and by the Swiss National Science Foundation.
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Haiqing Hua and Viola Günther contributed equally to this work.
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Hua, H., Günther, V., Georgiev, O. et al. Distorted copper homeostasis with decreased sensitivity to cisplatin upon chaperone Atox1 deletion in Drosophila . Biometals 24, 445–453 (2011). https://doi.org/10.1007/s10534-011-9438-1
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DOI: https://doi.org/10.1007/s10534-011-9438-1