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The use of RAPD markers for detecting genetic diversity, relationship and molecular identification of Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in a tea germplasm repository

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Abstract.

The genetic diversity, relationship and molecular identification of 15 well known, widely planted traditional Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in the China National Germplasm Hangzhou Tea Repository in the Tea Research Institute of the Chinese Academy of Agricultural Sciences located in Zhejiang province, China, were investigated using RAPD markers. A total of 1050 bands with an average of 52.5 bands per primer, 70 bands per genetic resource were generated by the 20 selected primers from the 15 tea genetic resources. In the total of 137 amplified products, 129 were polymorphic, corresponding to 94.2% genetic diversity. The relative frequency of polymorphic products was from 0.24 to 0.83, with an average of 0.47. In general, this average frequency was relatively high. The genetic distances among the genetic resources were from 0.16 to 0.62, with an average of 0.37. The 15 tea genetic resources were grouped into three groups by UPGMA cluster analysis based on RAPD data. By using the presence of 20 unique RAPD markers and the absence of 11 unique markers, all the 15 investigated tea genetic resources could be easily identified. RAPD markers provided a practical method not only to evaluate the genetic diversity and relationship, but also to identify tea genetic resources.

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References

  1. A. Belaj I. Trujilo R. Rosa L. Rallo M.J. Gimenez (2001) ArticleTitlePolymorphism and discrimination capacity of randomly amplified polymorphic markers in an olive germplasm bank Journal of the American Society for Horticultural Science 126 IssueID1 64–71 Occurrence Handle1:CAS:528:DC%2BD3MXjsFektw%3D%3D

    CAS  Google Scholar 

  2. B.B. Borse J.M. Rao S. Nagalakshmi N. Krishnamurthy (2002) ArticleTitleFingerprint of black teas from India: identification of regio-specific characteristics Food Chemistry 79 419–424 Occurrence Handle1:CAS:528:DC%2BD38XotFOjsrg%3D

    CAS  Google Scholar 

  3. L. Chen D.M. Chen Q.K. Gao Y.J. Yang F.L. Yu (1997) ArticleTitleIsolation and Appraisal of Genomic DNA from Tea Plant [Camellia sinensis (L) O Kuntze] Journal of Tea Science 17 IssueID2 177–181 Occurrence Handle1:CAS:528:DyaK1cXmslCksw%3D%3D

    CAS  Google Scholar 

  4. L. Chen Q.K. Gao Y.J. Yang F.L. Yu D.M. Chen (1998) ArticleTitleOptimum amplification procedure and reaction system for RAPD analysis of tea plants [Camellia sinensis (L.) O. Kuntze] Journal of Tea Science 18 IssueID1 16–20

    Google Scholar 

  5. L. Chen S. Yamaguchi (2002) ArticleTitleGenetic diversity and phylogeny of tea plant (Camellia sinensis) and its related species and varieties in the section Thea genus Camellia determined by randomly amplified polymorphic DNA analysis Journal of Horticultural Science and Biotechnology 77 IssueID6 729–732 Occurrence Handle1:CAS:528:DC%2BD3sXpsVyg

    CAS  Google Scholar 

  6. L. Chen F.L. Yu Y.J. Yang D.M. Chen C.J. Xu Q.K. Gao (1999) ArticleTitleA study on genetic stability of excellent tea germplasms [Camellia sinensis (L.) O. Kuntze] using RAPD markers Journal of Tea Science 19 IssueID1 11–13 Occurrence Handle1:CAS:528:DyaK1MXlvVWhsLY%3D

    CAS  Google Scholar 

  7. P.J. Conner B.W. Wood (2001) ArticleTitleIdentification of pecan cultivars and their genetic relatedness as determined by randomly amplified polymorphic DNA analysis Journal of the American Society for Horticultural Science 126 IssueID4 474–480 Occurrence Handle1:CAS:528:DC%2BD3MXltFehsLo%3D

    CAS  Google Scholar 

  8. A.L. Dhanaraj E.V.V.B. Rao K.R.M. Swamy M.G. Bhat D.T. Parsad S.N. Sondur (2002) ArticleTitleUsing RAPDs to assess the diversity in India cashew (Anacarditum occidentale L.) germplasm Journal of Horticultural Science and Biotechnology 77 IssueID1 41–47 Occurrence Handle1:CAS:528:DC%2BD38XitFOkur0%3D

    CAS  Google Scholar 

  9. U. Galderisi M. Cipollaro G.D. Bernardo L.D. Masi G. Galano A. Cascino (1999) ArticleTitleIdentification of hazelnut (Corylus avellana) cultivars by RAPD analysis Plant Cell Reports 18 652–655 Occurrence Handle1:CAS:528:DyaK1MXitVOlsrk%3D

    CAS  Google Scholar 

  10. C.A. Hackett F.N. Wachira S. Paul W. Powell R. Waugh (2000) ArticleTitleConstruction of a genetic linkage map for Camellia sinensis (tea) Heredity 85 IssueID4 346–355 Occurrence Handle1:CAS:528:DC%2BD3MXkvFWkug%3D%3D Occurrence Handle11122412

    CAS  PubMed  Google Scholar 

  11. M. Hashimoto S. Takasi (1978) ArticleTitleMorphological studies on the origin of the tea plant V: a proposal of one place of origin by cluster analysis Japanese Journal of Tropical Agriculture 21 93–101

    Google Scholar 

  12. J. Hu C.F. Quiros (1991) ArticleTitleIdentification of broccoli and cauliflower cultivars with RAPD markers Plant Cell Reports 10 505–511

    Google Scholar 

  13. S.S. Kaundun A. Zhyvoloup Y.G. Park (2000) ArticleTitleEvaluation of the genetic polymorphism among elite tea (Camellia sinensis var sinensis) accessions using RAPD markers Euphytica 115 7–16 Occurrence Handle1:CAS:528:DC%2BD3cXntFKqsrc%3D

    CAS  Google Scholar 

  14. P.D. Khasa B.P. Dancik (1996) ArticleTitleRapid identification of white-Engelmann spruce species by RAPD markers Theoretical and Applied Genetics 92 IssueID1 46–52 Occurrence Handle1:CAS:528:DyaK28Xlt1Cmu7Y%3D

    CAS  Google Scholar 

  15. B.S. Landry R.Q. Li W.Y. Cheung R.L. Granger (1994) ArticleTitlePhylogeny analysis of 25 apple rootstocks using RAPD markers and tactical gene tagging Theoretical and Applied Genetics 89 847–852 Occurrence Handle1:CAS:528:DyaK2MXkt1emtr8%3D

    CAS  Google Scholar 

  16. S.H. Lee H.S. Choi R.H. Kim H.Y. Lee I.S. Nou (1995) ArticleTitleIdentification of Korean wild tea plants and Japanese green tea cultivars using RAPD markers Journal of the Korean Tea Society 1 IssueID1 129–148

    Google Scholar 

  17. C.Y. Lu W.H. Liu M.J. Li (1992) ArticleTitleRelationship between the evolutionary relatives and the variation of esterase isozymes in tea plant Journal of Tea Science 12 IssueID1 15–20

    Google Scholar 

  18. R.K. Mirshra S. Sen-Mandi (2001) Genome analysis and isozyme studies for developing molecular markers associated with drought tolerance in tea plant Proceedings of 2001 International Conference on O-Cha (Tea) Culture and Science (Session II), 5–8 October 2001 Shizuoka Japan 66–69

    Google Scholar 

  19. T.K. Mondal P.K. Chand (2002) ArticleTitleDetection of genetic variation among micropropagated tea [Camellia sinensis (L.) O. Kuntze] by RAPD analysis In Vitro Cellular and Development Biology-Plant 38 296–299 Occurrence Handle1:CAS:528:DC%2BD38XmsFags7c%3D

    CAS  Google Scholar 

  20. M. Nei W.H. Li (1979) ArticleTitleMathematical model for studying genetic variation in terms of restriction endonuclease Proceedings of the National Academy of Science USA 76 IssueID10 5269–5273 Occurrence Handle1:CAS:528:DyaL3cXitVWn

    CAS  Google Scholar 

  21. M.J. Oh (1994) Studies on genetic relationship among the Korean native tea trees and physico-chemical properties of its green tea Department of AgricultureKorean University Korea 27–29

    Google Scholar 

  22. C. Orozco-Castillo K.J. Chalmers W. Powell R. Waugh (1996) ArticleTitleRAPD and organelle specific PCR re-affirms taxonomic relationships within the genus Coffea Plant Cell Reports 15 IssueID5 337–341 Occurrence Handle1:CAS:528:DyaK28XjtVeis7w%3D

    CAS  Google Scholar 

  23. Y.G. Park S.S. Kaundun A. Zhyvoloup (2002) ArticleTitleUse of the bulked genomic DNA-based RAPD methodology to access the genetic diversity among abandoned Korean tea plantations Genetic Resources and Crop Evolution 49 IssueID2 159–165

    Google Scholar 

  24. M.H. Sedra P. Lasherms P. Trouslot M. Combes S. Hamon (1998) ArticleTitleIdentification and genetic diversity analysis of date palm (Phoenix dactylifera L.) varieties from Morocco using RAPD markers Euphytica 103 75–82 Occurrence Handle1:CAS:528:DyaK1cXmvFantbo%3D

    CAS  Google Scholar 

  25. J.L. Shu L. Chen H.S. Wang P.S. Wang M. Xu W.X. Song (1998) ArticleTitlePollen morphology, ultrastructure and evolution of tea plants and other genus Camellia plants Journal of Tea Science 18 IssueID1 7–17

    Google Scholar 

  26. Y. Takeda (2000) History and development in Japanese tea breeding Y.G. Park D.I. Shin (Eds) Tea CultureTea Food Industry and Tea Breeding in KoreaChina and Japan The Korea Tea Society Korea 139–158

    Google Scholar 

  27. T. Takeo X.Q. You H.F. Wang H. Kinukasa M.J. Li Q.K. Cheng H.S. Wang (1992) ArticleTitleOne speculation on the origin and dispersion of tea plant in China – One speculation based on the chemotaxonomy by using the contest-ration of terpene – alcohols found in the aroma composition Journal of Tea Science 12 IssueID2 81–86

    Google Scholar 

  28. J. Tanaka S. Yamaguchi (1996) ArticleTitleUse of RAPD markers for the identification of parentage of tea cultivars Bulletin of the National Institute of Vegetables, Ornamental Plants & TeaJapan 9 IssueIDB 31–36

    Google Scholar 

  29. J. Tanaka N. Yamaguchi Y. Nakamura (2001) ArticleTitlePollen parent of tea cultivar Sayamakaori with insect and cold resistance may not exist Breeding Research 3 43–48

    Google Scholar 

  30. Q.Y. Tang M.G. Feng (1997) Practical Statistics and DPS Data Processing System China Agricultural Press Beijing p. 407

    Google Scholar 

  31. F.N. Wachira W. Powell R. Waugh (1997) ArticleTitleAn assessment of genetic diversity among Camellia sinensis L. (cultivated tea) and its wild relatives based on randomly amplified polymorphic DNA and organelle-specific STS Heredity 78 603–611 Occurrence Handle1:CAS:528:DyaK2sXks1Sjs7w%3D

    CAS  Google Scholar 

  32. F.N. Wachira R. Waugh C.A. Hackett W. Powell (1995) ArticleTitleDetection of genetic diversity in tea (Camellia sinensis) using RAPD markers Genome 38 201–210 Occurrence Handle1:CAS:528:DyaK2MXmsV2itrs%3D Occurrence Handle7774794

    CAS  PubMed  Google Scholar 

  33. Y. Wang F.Y. Lin L.M. Shi (1994) ArticleTitleInvestigation on the mtDNA polymorphism from the natural population of Drosophila sp, (II) Origin and differentiation of Drosophila sp Acta Genetica Sinica 21 IssueID4 263–274 Occurrence Handle1:CAS:528:DyaK2cXksFSjtLo%3D

    CAS  Google Scholar 

  34. X.Q. Wang Y.P. Zou D.M. Zhang D.Y. Hong (1996) ArticleTitleRAPD analysis on genetic polymorphism of silvery fir Science in China Series C 26 436–444

    Google Scholar 

  35. J.G.K. Williams A.R. Kubelik K.J. Livak J.A. Rafalski S.V. Tingey (1990) ArticleTitleDNA polymorphisms amplified by arbitrary primers are useful as genetic markers Nucleic Acids Research 18 IssueID22 6531–6535 Occurrence Handle1:CAS:528:DyaK3MXjslWmsA%3D%3D Occurrence Handle1979162

    CAS  PubMed  Google Scholar 

  36. F.L. Yu (1986) ArticleTitleDiscussion on the originating place and the originating center of tea plant Journal of Tea Science 6 IssueID1 1–8

    Google Scholar 

Download references

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Authors and Affiliations

  1. Laboratory for Germplasm, Breeding and Molecular Biology, Tea Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, 1 Yunqi Road, Hangzhou, Zhejiang, 310008, China

    Liang Chen

  2. Biological Macromolecular Research Laboratory of Analyzing and Measurement Center, China

    Qi-kang Gao

  3. Department of Horticulture, Zhejiang University, 268 Kaixuan Road, Hangzhou, Zhejiang, 310029, China

    Da-ming Chen & Chang-jie Xu

Authors
  1. Liang Chen
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  2. Qi-kang Gao
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  3. Da-ming Chen
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  4. Chang-jie Xu
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Correspondence to Liang Chen.

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Chen, L., Gao, Qk., Chen, Dm. et al. The use of RAPD markers for detecting genetic diversity, relationship and molecular identification of Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in a tea germplasm repository. Biodivers Conserv 14, 1433–1444 (2005). https://doi.org/10.1007/s10531-004-9787-y

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  • DOI: https://doi.org/10.1007/s10531-004-9787-y

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