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Recovery of succinic acid from fermentation broth

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Abstract

Succinic acid is of high interest as bio-feedstock for the chemical industry. It is a precursor for a variety of many other chemicals, e.g. 1,4-butandiol, tetrahydrofuran, biodegradable polymers and fumaric acid. Besides optimized production strains and fermentation processes it is indispensable to develop cost-saving and energy-effective downstream processes to compete with the current petrochemical production process. Various methods such as precipitation, sorption and ion exchange, electrodialysis, and liquid–liquid extraction have been investigated for the recovery of succinic acid from fermentation broth and are reviewed critically here.

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References

  • Baniel AM, Eyal AM (1995) Citric acid extraction. US patent 5,426,220

  • Bechthold I, Bretz K, Kabasci S, Kopitzky R, Springer A (2008) Succinic acid: a new platform chemical for biobased polymers from renewable resources. Chem Eng Technol 5:647–654

    Article  Google Scholar 

  • Berglund KA, Elankovan P, Glassner DA (1991) Carboxylic acid purification and crystallization process. US patent 5,034,105

  • Berglund KA, Yedur S, Dunuwila D (1999) Succinic acid production and purification. US patent 5,958,744

  • Cascaval D, Balaction AI (2004) New separation techniques on bioseparations 1. Reactive extraction. Chem Ind 58(9):375–386

    CAS  Google Scholar 

  • Cornils B, Lappe P (2002) Dicarboxylic acids, aliphatic Ullmann’s Enzyklopedia of industrial chemistry. Wiley-VCH Verlag, Weinheim

    Google Scholar 

  • Datta R (1992) Process for the production of succinic acid by anaerobic fermentation. US patent 5,143,833

  • Datta R, Glassner DA, Jain MK, Vicky Roy JR (1992) Fermentation and purification process for succinic acid. US patent 5,168,055

  • Delhomme C, Weuster-Botz D, Kühn F (2009) Succinic acid from renewable resources as a C4 building-block chemical–a review of the catalytic possibilities in aqueous media. Green Chem 11:13–26

    Article  CAS  Google Scholar 

  • Glassner DA, Satinwood RD, Datta R (1992) Process for the production and purification of succinic acid. US patent 5,143,834

  • Hermann BG, Patel M (2007) Today’s and tomorrow’s bio-based bulk chemicals from white biotechnology–a techno-economic analysis. Appl Biochem Biotechnol 136:361–388

    Article  CAS  PubMed  Google Scholar 

  • Heyberger A, Prochazka J, Volaufova E (1997) Extraction of citric acid with tertiary amine–third phase formation. Chem Eng Sci 97:00321–00327

    Google Scholar 

  • Hong YK, Hong WH (2000a) Equilibrium studies on reactive extraction of succinic acid from aqueous solutions with tertiary amines. Bioproc Eng 22:477–481

    Article  CAS  Google Scholar 

  • Hong YK, Hong WH (2000b) Reactive extraction of succinic acid with tripropylamine (TPA) in various diluents. Bioproc Eng 22:281–284

    Article  CAS  Google Scholar 

  • Hong YK, Hong WH (2000c) Extraction of succinic acid with 1-octanol/n-heptane solutions of mixed tertiary amine. Bioproc Eng 23:535–538

    Article  CAS  Google Scholar 

  • Hong YK, Hong WH (2004) Influence of chain length of tertiary amines on extractability and chemical interactions in reactive extraction of succinic acid. Korean J Chem Eng 21:488–493

    Article  CAS  Google Scholar 

  • Hong SH, Lee SY (2001) Metabolic flux analysis for succinic acid production by recombinant Escherichia coli with amplified malic enzyme activity. Biotechnol Bioeng 74:89–95

    Article  CAS  PubMed  Google Scholar 

  • Hong YK, Hong WH, Han DH (2001) Application of reactive extraction to recovery of carboxylic acids. Biotechnol Bioproc Eng 6:386–394

    Article  CAS  Google Scholar 

  • Hong YK, Han DH, Hong WH (2002) Water enhanced solubilities of succinic acid in reactive extraction using tertiary amines/alcohol systems. Korean J Chem Eng 19:83–86

    Article  CAS  Google Scholar 

  • Huh YS, Hong YK, Hong WH, Chang HN (2004) Selective extraction of acetic acid from the fermentation broth produced by Mannheimia succiniciproducens. Biotechnol Lett 26:1581–1584

    Article  CAS  PubMed  Google Scholar 

  • Huh YS, Jun Y-S, Kong YK, Song H, Lee SY, Hong WH (2006) Effective purification of succinic acid from fermentation broth produced by Mannheimia succiniciproducens. Proc Biochem 41:1461–1465

    Article  CAS  Google Scholar 

  • Jaquet A, Quan R, Marison W, vain Stockar U (1999) Factors influenceing in potentioluse of aliquat 336 for the in situ extraction of carboxylic acids from cultures of Pseudomonas putida. J Biotechnol 68:185

    Article  CAS  PubMed  Google Scholar 

  • Juang RS, Huang RH (1997) Equilibrium studies on reactive extraction of lactic acid with an amine extractant. Chem Eng J 65:47–53

    Article  CAS  Google Scholar 

  • Jun YS, Huh YS, Park HS, Thomas A, Jeon SJ, Lee EZ, Won HJ, Hong WH, Lee SY, Hong YK (2007) Adsorption of pyruvic and succinic acid by amine-functionalized SBA-15 for the purification of succinic acid from fermentation broth. J Phys Chem C 200(111):13076–13086

    Article  Google Scholar 

  • Kahya E, Bayraktar E, Mehmetoglu Ü (2001) Optimization of process parameters for reactive lactic acid extraction. Turk J Chem 25:223–230

    CAS  Google Scholar 

  • Kertes AS, King CJ (1985) Extraction chemistry of fermentation product carboxylic acids. Biotechnol Bioeng 28:269

    Article  Google Scholar 

  • Kim BS, Hong YK, Hong WH (2002) Effect of pH on the extraction characteristics of succinic acid and the stability of colloidal liquid aphrons. Korean J Chem Eng 19:669–672

    Article  CAS  Google Scholar 

  • Kim BS, Hong YK, Hong WH (2004a) Effect of salts on the extraction characteristics of succinic acid by predispersed solvent extraction. Biotechnol Bioproc Eng 9:207–211

    Article  CAS  Google Scholar 

  • Kim BS, Hong YK, Huh YS, Hong WH (2004b) Predispersed solvent extraction of succinic acid aqueous solution by colloidal liquid aphrons in column. Biotechnol Bioproc Eng 9:454–458

    Article  CAS  Google Scholar 

  • King CJ, Dtarr J (1990) Recovery of carboxylic acids from water by precipitation from organic solutions. US patent 5,104,492

  • King CJ, Poole LJ (1993) Craboxylic acid sorption regeneration process. US patent 5,412,126

  • King CJ, Sanchez PA (1985) Process for sorption solute recovery. US patent 4,670,155

  • Kirk-Othmer (1979) Encyclopedia of chemical technology, 3rd edn. Wiley, New York. Citric acid, vol 6; Hxdroxycarboxylic acids, vol 13

  • Kushiku T, Fujiwara K, Satou T, Sano (2006) Method for purifying succinic acid from fermentation broth. US patent 2006/0276674A1

  • Lee SY, Kim JM, Song H, Lee JW, Kim TY, Yang YS (2008) From genome sequence to integrated bioprocess for succinic acid production by Mannheimia succiniciproducens. Appl Microbiol Biotechnol 79:11–22

    Article  CAS  PubMed  Google Scholar 

  • Li Q, Li WL, Wang D, Liu BB, Tang H, Yang MH, Liu Q, Xing JM, Su ZG (2008) pH neutralization while succinic acid adsorption onto anion-exchange resins. Appl Biochem Biotechnol. doi:10.1007/s12010-008-8355-4

  • Likidis Z, Schügerl K (1987) Recovery of penicillin by reactive extraction in centrifugal extractors. Biotechnol Bioeng 30:1032–1040

    Article  CAS  PubMed  Google Scholar 

  • Matsumoto M, Otono T, Kondo K (2001) Synergistic extraction of organic acids with tri-n-octylamine ant tri-n-butylphosphate. Sep Purif Technol 24:337–342

    Article  CAS  Google Scholar 

  • McKinlay JB, Vieille C, Zeikus JG (2007) Prospects for a bio-based succinate industry. Appl Microbiol Biotechnol. doi:10.1007/s00253-007-1057-y

  • Meyinal-Salles I, Dorotyn S, Soucaille P (2007) A new process for the continuous production of succinic acid from glucose at high yield, titer, an productivity. Biotechnol Bioeng 99(1) Jan 1, 2008

  • Okino SH, Noburyu R, Suda M, Jojima T, Inui M, Yukawa H (2008) An efficient succinic acid production process in a metabolically engineered Corynebacterium glutamicum strain. Appl Microbiol Biotechnol 81:459–464

    Article  CAS  PubMed  Google Scholar 

  • Pai RA, Doherty MF, Malone MF (2002) Design of reactive extraction systems for bioproduct recovery. AIChE J 48:514–526

    Article  CAS  Google Scholar 

  • Patel MA (2006) Medium and long-term opportunities and risks of the biotechnological production of bulk chemicals from renewable resources—the potential of white biotechnology. The BREW Project (http:www.chem.uu.nl/brew/)

  • Pazouki M, Panda T (1998) Recovery of citric acid—a review. Bioproc Eng 19:435–439

    Article  CAS  Google Scholar 

  • Poole LJ, King CJ (1991) Regeneration of carboxylic acid-amine extracts by back-extraction with an aqueous solution of a volatile amine. Ind Eng Chem Res 30:923–929

    Article  CAS  Google Scholar 

  • Rüffer N, Heidersdorf U, Kretzers I, Sprenger GA, Raeven L, Takors R (2004) Fully integrated L-phenylalanine separation and concentration using reactive-extraction with liquid-liquid centrifuges in a fed-batch process with E. coli. Bioprocess Biosyst Eng 26:239–248

    Article  PubMed  Google Scholar 

  • Schlichting E, Halwachst W, Schügerl K (1987) Reactive extraction of salicylic acid and D/L phenylalanine in a stirred cell. Chem Eng Com 51:193–205

    Article  CAS  Google Scholar 

  • Song H, Lee SY (2005) Production of succinic acid by bacterial fermentation. Enzym Microbial Technol 39:352–361

    Article  Google Scholar 

  • Song H, Huh YS, Lee SY, Hong WH, Hong YK (2007) Recovery of succinic acid produced by fermentation of a metabolomically engineered Mannheimia succiniciproducens strain. J Biotechnnol 132:445–452

    Article  CAS  Google Scholar 

  • Tamada JA, King CJ (1990a) Extraction of carboxylic acids with amine extractants: 2. Chemical interactions and interpretation of data. Ind Eng Chem Res 29:1327–1333

    Article  CAS  Google Scholar 

  • Tamada JA, King CJ (1990b) Extraction of carboxylic acids with amine extractants: 3. Effects of temperature, water coextraction and process considerations. Ind Eng Chem Res 29:1333–1338

    Article  CAS  Google Scholar 

  • Tamada JA, Kertes AS, King CJ (1990) Extraction of carboxylic acids with amine extractants: 1. Equilibria and law of mass action modeling. Ind Eng Chem Res 29:1319–1326

    Article  CAS  Google Scholar 

  • Tung LA, King CJ (1994a) Sorption and extraction of lactic and succinic acids at pH > pKa1. 1. Factors governing equilibria. Ind Eng Chem Res 33:3217–3223

    Article  CAS  Google Scholar 

  • Tung LA, King CJ (1994b) Sorption and extraction of lactic and succinic acids at pH > pKa1. 2. Regeneration and process considerations. Ind Eng Chem Res 33:3224–3229

    Article  CAS  Google Scholar 

  • Vickroy TB (1985) Lactic acid. In: Comprehensive Biotechnology, Blanch HW, Drew S, Wang DIC (eds) Comprehensive biotechnology, vol 3, pp 761–776. Pergamon Press, New York

  • Wieczorek S, Bauer H (1998) Continuous production of citric acid with recirculation of the fermentation broth after product recovery. Bioprocess Eng 18:1–5

    Article  CAS  Google Scholar 

  • Wilke T, Vorlop K-D (2004) Industrial bioconversion of renewable resources as an alternative to conventional chemistry. Appl Microbiol Biotechnol 66:131–142

    Article  Google Scholar 

  • Yedur S, Berglung KS, Dunuwila DD (2001) Succinic acid production and purification. US patent 6,265,190

  • Zang JA, Moshy RJ, Smith RN (1966) Electrodialysis in food processing. Chem Eng Progress Symp Ser 62:105–110

    CAS  Google Scholar 

  • Zeikus JG, Jain MK, Elankovan P (1999) Biotechnology of succinic acid production and markets for derived industrial products. Appl Microbiol Biotechnol 51:545–552

    Article  CAS  Google Scholar 

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Acknowledgments

This work was generously supported by the Deutsche Bundesstiftung Umwelt (DBU), particularly by a DBU Ph.D. grant for Tanja Kurzrock.

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  1. Institute of Biochemical Engineering, Technische Universität München, Boltzmannstr. 15, 85748, Garching, Germany

    Tanja Kurzrock & Dirk Weuster-Botz

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  1. Tanja Kurzrock
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  2. Dirk Weuster-Botz
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Correspondence to Dirk Weuster-Botz.

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Kurzrock, T., Weuster-Botz, D. Recovery of succinic acid from fermentation broth. Biotechnol Lett 32, 331–339 (2010). https://doi.org/10.1007/s10529-009-0163-6

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  • DOI: https://doi.org/10.1007/s10529-009-0163-6

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