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Simultaneous degradation of phytic acid and starch by an industrial strain of Saccharomyces cerevisiae producing phytase and α-amylase

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Abstract

Phytase liberates inorganic phosphate from phytic acid (myo-inositol hexakisphosphate) which is the major phosphate reserve in plant-derived foods and feeds. An industrial strain of Saccharomyces cerevisiae expressing the Debaryomyces castellii phytase gene (phytDc) and D. occidentalis α-amylase gene (AMY) was developed. The phytDc and AMY genes were constitutively expressed under the ADC1 promoter in S. cerevisiae by using the δ-integration system, which contains DNA derived exclusively from yeast. The recombinant industrial strain secreted both phytase and α-amylase for the efficient degradation of phytic acid and starch as main components of plant seeds. This new strain hydrolyzed 90% of 0.5% (w/v) sodium phytate within 5 days of growth and utilized 100% of 2% (w/v) starch within 48 h simultaneously.

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Acknowledgement

Mi-Hyeon Lim and Ok-Hee Lee were supported by the second stage of the Brain Korea 21 project.

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Authors and Affiliations

  1. Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju, 500-757, South Korea

    Mi-Hyeon Lim, Ok-Hee Lee, Hyun-Mi Ko, Il-Chul Kim, Hwanghee Blaise Lee, Suhn-Young Im & Suk Bai

  2. Department of Cosmetology, Dongkang College, Gwangju, South Korea

    Jong-Eon Chin

Authors
  1. Mi-Hyeon Lim
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  2. Ok-Hee Lee
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  3. Jong-Eon Chin
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  4. Hyun-Mi Ko
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  5. Il-Chul Kim
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  6. Hwanghee Blaise Lee
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  7. Suhn-Young Im
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  8. Suk Bai
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Correspondence to Suk Bai.

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Lim, MH., Lee, OH., Chin, JE. et al. Simultaneous degradation of phytic acid and starch by an industrial strain of Saccharomyces cerevisiae producing phytase and α-amylase. Biotechnol Lett 30, 2125–2130 (2008). https://doi.org/10.1007/s10529-008-9799-x

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  • DOI: https://doi.org/10.1007/s10529-008-9799-x

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