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Phytase expression in transgenic soybeans: stable transformation with a vector-less construct

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Abstract

A minimal linear gene cassette (35S-phytase gene-nos) with T-DNA borders was acquired by PCR and directly introduced into soybean through the pollen tube pathway. A total of 13% of T1 plants were positive for phyA by specific PCR. Southern blot analyses showed that phyA insertions were harbored stably in T2 progeny. Phytase expression level increased 2.5-fold over a 6-week period; its highest activity was 150 U/mg protein, compared to 56 U/mg protein in untransformed controls. Activity of phytase increased to 125 FTU/kg in T3 transgenic seeds as compared to 64 FTU/kg in wild-type plants.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of the Dalian Government in China (2005J22JH044).

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian, 116024, P.R. China

    Xiao Rong Gao, Guo Kun Wang, Qiao Su, Yan Wang & Li Jia An

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  1. Xiao Rong Gao
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  2. Guo Kun Wang
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  3. Qiao Su
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  4. Yan Wang
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  5. Li Jia An
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Correspondence to Xiao Rong Gao.

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Gao, X.R., Wang, G.K., Su, Q. et al. Phytase expression in transgenic soybeans: stable transformation with a vector-less construct. Biotechnol Lett 29, 1781–1787 (2007). https://doi.org/10.1007/s10529-007-9439-x

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  • DOI: https://doi.org/10.1007/s10529-007-9439-x

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