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Plasma protein oxidation in aging rats after alpha-lipoic acid administration

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Abstract

In the present study, we investigated whether alpha-lipoic acid administration could have prooxidant or antioxidant effect on oxidative protein damage parameters such as protein carbonyl, nitrotyrosine, advanced oxidation protein products, and protein thiol, as well as oxidative stress parameters such as total thiol, nonprotein thiol, and lipid hydroperoxide in the plasma proteins of aged rats. Alpha-lipoic acid (100 mg/kg body wt/day) was administrated intraperitoneally to the Sprague-Dawley rats for 14 days. Protein carbonyl, nitrotyrosine, and advanced oxidation protein products levels were increased, protein thiol, nonprotein thiol, and total thiol levels were not changed in the plasma proteins of aged rats with alpha-lipoic acid administration. In aging rats with and without alpha-lipoic acid administration, plasma lipid hydroperoxide levels were significantly increased compared with those of the control group. The increased levels of protein oxidation markers such as protein carbonyl, nitrotyrosine and advanced oxidation protein products in the plasma proteins of alpha-lipoic acid-administrated aged rats compared with nonadministrated aged rats suggests that protein oxidation is increased in alpha-lipoic acid-administrated aged rats. We assume that an explanation for our findings regarding alpha-lipoic acid administration on protein oxidation markers in the plasma proteins of aged rats may be due to the prooxidant effects of alpha-lipoic acid.

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Abbreviations

AOPP:

advanced oxidation protein products

DHLA:

dihydrolipoic acid

LHP:

lipid hydroperoxide

NT:

nitrotyrosine

Np-SH:

nonprotein thiol

ONOO−:

peroxynitrite

PCO:

protein carbonyl

LA:

DL-alpha-lipoic acid

PO:

protein oxidation

PSH:

protein thiol

T-SH:

total thiol

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Authors and Affiliations

  1. Istanbul Faculty of Medicine, Central Laboratory of Biochemistry, Istanbul University, 34390, Capa, Istanbul, Turkey

    Ufuk Çakatay

  2. Cerrahpasa Faculty of Medicine, Department of Biochemistry, Istanbul University, 34303, Kocamustafapasa, Istanbul, Turkey

    Refik Kayaliı

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  1. Ufuk Çakatay
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  2. Refik Kayaliı
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Correspondence to Ufuk Çakatay.

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Çakatay, U., Kayaliı , R. Plasma protein oxidation in aging rats after alpha-lipoic acid administration. Biogerontology 6, 87–93 (2005). https://doi.org/10.1007/s10522-005-3462-x

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