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Analysis of Major Isoforms of Human Growth Hormone before and after Intensive Physical Exercise

  • Sports Medicine
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Bulletin of Experimental Biology and Medicine Aims and scope

We developed a semiquantitative method for the analysis of main growth hormone isoforms. The use of immunoaffinity sorbents, two-dimensional electrophoresis, and immunoblotting allows detection of more than 90% circulating growth hormone. It was demonstrated that the proportion of growth hormone isoforms in human serum before and after strenuous exercise remained unchanged.

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Authors and Affiliations

  1. Russian Physical Training and Sports Institute, Moscow, Russia

    D. A. Sakharov, M. Thevis & A. G. Tonevitsky

  2. Institute for Biochemistry, German Sport University, Cologne, Germany

    D. A. Sakharov, M. Thevis & A. G. Tonevitsky

Authors
  1. D. A. Sakharov
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  2. M. Thevis
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  3. A. G. Tonevitsky
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Corresponding author

Correspondence to D. A. Sakharov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 146, No. 10, pp. 446–450, October, 2008

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Sakharov, D.A., Thevis, M. & Tonevitsky, A.G. Analysis of Major Isoforms of Human Growth Hormone before and after Intensive Physical Exercise. Bull Exp Biol Med 146, 466–469 (2008). https://doi.org/10.1007/s10517-009-0335-3

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  • DOI: https://doi.org/10.1007/s10517-009-0335-3

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