Abstract
A Gram-staining-negative, aerobic, non-spore-forming, non-flagellated and rod- or short rod-shaped bacterial strain, designated BH-4T, was isolated from coastal seawater in the East Sea, South Korea. Strain BH-4T grew optimally at 25 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. Strain BH-4T exhibited the highest 16S rRNA gene sequence similarity value (96.5 %) to Hoeflea phototrophica NCIMB 14078T. It exhibited 94.2–95.5, 94.7 and 93.8 % sequence similarity values to the type strains of the other Hoeflea species, Ahrensia kielensis and Pseudahrensia aquimaris, respectively. Neighbor-joining, maximum-likelihood and maximum-parsimony phylogenetic trees revealed that strain BH-4T clustered with the type strains of A. kielensis and P. aquimaris. Strain BH-4T contained Q-10 as the predominant ubiquinone as shown in the genera Ahrensia, Pseudahrensia and Hoeflea. The fatty acid and polar lipid profiles of strain BH-4T were distinguished from those of members of the genera Ahrensia, Pseudahrensia and Hoeflea. The DNA G + C content was 49.3 mol%, lower than those of the type strains of P. aquimaris and Hoeflea species. The phylogenetic data, differential chemotaxonomic and other phenotypic properties revealed that strain BH-4T constitutes a new genus and species within the Alphaproteobacteria, for which the name Lentilitoribacter donghaensis gen. nov., sp. nov., is proposed. The type strain of Lentilitoribacter donghaensis is BH-4T (=KCTC 32082T = CCUG 62792T).
Similar content being viewed by others
References
Biebl H, Tindall BJ, Pukall R, Lünsdorf H, Allgaier M, Wagner-Döbler I (2006) Hoeflea phototrophica sp. nov., a novel marine aerobic alphaproteobacterium that forms bacteriochlorophyll a. Int J Syst Evol Microbiol 56:821–826
Bruns A, Rohde M, Berthe-Corti L (2001) Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006
Cowan ST, Steel KJ (1965) Manual for the identification of medical bacteria. Cambridge University Press, London
Jung YT, Park S, Lee JS, Oh TK, Yoon JH (2012) Pseudahrensia aquimaris gen. nov., sp. nov., isolated from seawater. Int J Syst Evol Microbiol 62:2056–2061
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721
Komagata K, Suzuki KI (1987) Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207
Krieg NR (2005) Genus II. Ahrensia. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol 2, part C, 2nd edn. Springer, New York, pp 167–168
Lányí B (1987) Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
Lee KB, Liu CT, Anzai Y, Kim H, Aono T, Oyaizu H (2005) The hierarchical system of the Alphaproteobacteria: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55:1907–1919
Leifson E (1963) Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
Minnikin DE, Patel PV, Alshamaony L, Goodfellow M (1977) Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117
Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241
Palacios L, Arahal DR, Reguera B, Marín I (2006) Hoeflea alexandrii sp. nov., isolated from the toxic dinoflagellate Alexandrium minutum AL1 V. Int J Syst Evol Microbiol 56:1991–1995
Peix A, Rivas R, Trujillo ME, Vancanneyt M, Velázquez E, Willems A (2005) Reclassification of Agrobacterium ferrugineum LMG 128 as Hoeflea marina gen. nov., sp. nov. Int J Syst Evol Microbiol 55:1163–1166
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. Microbial ID, Inc., Newark
Stevenson BS, Suflita MT, Stamps BW, Moore ER, Johnson CN, Lawson PA (2011) Hoeflea anabaenae sp. nov., an epiphytic symbiont that attaches to the heterocysts of a strain of Anabaena. Int J Syst Evol Microbiol 61:2439–2444
Tamaoka J, Komagata K (1984) Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128
Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Uchino Y, Hirata A, Yokota A, Sugiyama J (1998) Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44:201–210
Yoon JH, Kim H, Kim SB, Kim HJ, Kim WY, Lee ST, Goodfellow M, Park YH (1996) Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505
Yoon JH, Lee ST, Park YH (1998) Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194
Yoon JH, Kang KH, Park YH (2003) Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454
Yoon JH, Kang SJ, Lee SY (2012) Salinimonas lutimais sp. nov., a polysaccharide-degrading bacterium isolated from a tidal flat. Antonie Van Leeuwenhoek 101:803–810
Acknowledgments
This work was supported by the Program for Collection of Domestic Biological Resources from the National Institute of Biological Resources (NIBR) and BK 21 program from the Ministry of Education, Science and Technology (MEST) of the Republic of Korea.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Supplementary Fig. 1
Maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showing the positions of Lentilitoribacter donghaensis BH-4T and representatives of some other related taxa. Bootstrap values (expressed as percentages of 1000 replications) of >50 % are shown at branching points. Hyphomicrobium denitrificans DSM1869T was used as an outgroup. Bar, 0.01 substitutions per nucleotide position (PPT 127 kb)
Supplementary Fig. 2
Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences showing the positions of Lentilitoribacter donghaensis BH-4T and representatives of some other related taxa. Bootstrap values (expressed as percentages of 1000 replications) of >50 % are shown at branching points. Hyphomicrobium denitrificans DSM1869T was used as an outgroup. Bar, 0.01 substitutions per nucleotide position (PPT 128 kb)
Supplementary Fig. 3
Thin layer chromatogram of the total polar lipids of Lentilitoribacter donghaensis BH-4T. Spots were revealed by spraying the plates with 10 % ethanolic molybdophosphoric acid. Abbreviations: PG, phosphatidylglycerol; PME, phosphatidylmonomethylethanolamine; AL, unidentified aminolipid; PL, unidentified phospholipid (PPT 146 kb)
Rights and permissions
About this article
Cite this article
Park, S., Lee, JS., Lee, KC. et al. Lentilitoribacter donghaensis gen. nov., sp. nov., a slowly-growing alphaproteobacterium isolated from coastal seawater. Antonie van Leeuwenhoek 103, 457–464 (2013). https://doi.org/10.1007/s10482-012-9825-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10482-012-9825-9