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Lentilitoribacter donghaensis gen. nov., sp. nov., a slowly-growing alphaproteobacterium isolated from coastal seawater

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Abstract

A Gram-staining-negative, aerobic, non-spore-forming, non-flagellated and rod- or short rod-shaped bacterial strain, designated BH-4T, was isolated from coastal seawater in the East Sea, South Korea. Strain BH-4T grew optimally at 25 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. Strain BH-4T exhibited the highest 16S rRNA gene sequence similarity value (96.5 %) to Hoeflea phototrophica NCIMB 14078T. It exhibited 94.2–95.5, 94.7 and 93.8 % sequence similarity values to the type strains of the other Hoeflea species, Ahrensia kielensis and Pseudahrensia aquimaris, respectively. Neighbor-joining, maximum-likelihood and maximum-parsimony phylogenetic trees revealed that strain BH-4T clustered with the type strains of A. kielensis and P. aquimaris. Strain BH-4T contained Q-10 as the predominant ubiquinone as shown in the genera Ahrensia, Pseudahrensia and Hoeflea. The fatty acid and polar lipid profiles of strain BH-4T were distinguished from those of members of the genera Ahrensia, Pseudahrensia and Hoeflea. The DNA G + C content was 49.3 mol%, lower than those of the type strains of P. aquimaris and Hoeflea species. The phylogenetic data, differential chemotaxonomic and other phenotypic properties revealed that strain BH-4T constitutes a new genus and species within the Alphaproteobacteria, for which the name Lentilitoribacter donghaensis gen. nov., sp. nov., is proposed. The type strain of Lentilitoribacter donghaensis is BH-4T (=KCTC 32082T = CCUG 62792T).

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Acknowledgments

This work was supported by the Program for Collection of Domestic Biological Resources from the National Institute of Biological Resources (NIBR) and BK 21 program from the Ministry of Education, Science and Technology (MEST) of the Republic of Korea.

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Correspondence to Jung-Hoon Yoon.

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Supplementary Fig. 1

Maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showing the positions of Lentilitoribacter donghaensis BH-4T and representatives of some other related taxa. Bootstrap values (expressed as percentages of 1000 replications) of >50 % are shown at branching points. Hyphomicrobium denitrificans DSM1869T was used as an outgroup. Bar, 0.01 substitutions per nucleotide position (PPT 127 kb)

Supplementary Fig. 2

Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences showing the positions of Lentilitoribacter donghaensis BH-4T and representatives of some other related taxa. Bootstrap values (expressed as percentages of 1000 replications) of >50 % are shown at branching points. Hyphomicrobium denitrificans DSM1869T was used as an outgroup. Bar, 0.01 substitutions per nucleotide position (PPT 128 kb)

Supplementary Fig. 3

Thin layer chromatogram of the total polar lipids of Lentilitoribacter donghaensis BH-4T. Spots were revealed by spraying the plates with 10 % ethanolic molybdophosphoric acid. Abbreviations: PG, phosphatidylglycerol; PME, phosphatidylmonomethylethanolamine; AL, unidentified aminolipid; PL, unidentified phospholipid (PPT 146 kb)

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Park, S., Lee, JS., Lee, KC. et al. Lentilitoribacter donghaensis gen. nov., sp. nov., a slowly-growing alphaproteobacterium isolated from coastal seawater. Antonie van Leeuwenhoek 103, 457–464 (2013). https://doi.org/10.1007/s10482-012-9825-9

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  • DOI: https://doi.org/10.1007/s10482-012-9825-9

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