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Cell Attachment–Detachment Control on Temperature-Responsive Thin Surfaces for Novel Tissue Engineering

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Abstract

Temperature-responsive intelligent surfaces, prepared by the modification of an interface mainly with poly(N-isopropylacrylamide) and its derivatives, have been investigated. Such surfaces exhibit temperature-responsive hydrophilic/hydrophobic alterations with external temperature changes, which, in turn, result in thermally modulated attachment and detachment with cells. The advantage of this system is that cells cultured on such temperature-responsive surfaces can be recovered as single cells and/or confluent cell sheets, while keeping the deposited extracellular matrix intact, simply by lowering the temperature without conventional enzymatic treatment. Here, we focus and compare various methods of producing temperature-responsive surfaces for controlling cell attachment/detachment. Spontaneous cell attachment and detachment using several types of temperature-responsive surfaces are mentioned and various effects, such as film thickness and polymer conformation, are discussed. In addition, the development of the next generation of temperature-responsive surfaces using modifications of the polymer coating to allow for rapid cell recovery is summarized.

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Abbreviations

AAc:

Acrylic acid

AFM:

Atomic force microscopy

ATR-FTIR:

Attenuated total reflection-Fourier transform

ATRP:

Atom transfer radical polymerization

CIPAAm:

2-Carboxyisopropylacrylamide

CS:

Coverslips

EB:

Electron beam

ECM:

Extra cellular matrix

EC:

Bovine aortic endothelial cell

EDC:

1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide

FN:

Fibronectin

HPDL:

Human periodontal ligament cells

HUVEC:

Human umbilical vein endothelial cells

IPAAm:

N-isopropylacrylamide

LCST:

Lower critical solution temperature

MDCK:

Madin-Darby canine kidney

NHS:

N-hydroxysuccinimide

PEG:

Poly(ethylene glycol)

RCO:

Rat calvarial osteoblasts

RGD:

Arg-Gly-Asp

RGDS:

Arg-Gly-Asp-Ser

TCPS:

Tissue Culture Polystyrene

ToF-SIMS:

Time of flight secondary ion mass spectrometer

XPS:

X-ray photoelectron spectroscopy

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Acknowledgments

The present research was financially supported by Formation of Innovation Center for fusion of Advanced Technologies in the Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. The authors are grateful to Dr. N. Ueno (Tokyo Women’s Medical University) for her valuable comments and suggestions.

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  1. Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawadacho, Shinjuku, Tokyo, 162-8666, Japan

    Yoshikazu Kumashiro, Masayuki Yamato & Teruo Okano

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  1. Yoshikazu Kumashiro
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  2. Masayuki Yamato
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  3. Teruo Okano
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Correspondence to Teruo Okano.

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Associate Editor Michael S. Detamore oversaw the review of this article.

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Kumashiro, Y., Yamato, M. & Okano, T. Cell Attachment–Detachment Control on Temperature-Responsive Thin Surfaces for Novel Tissue Engineering. Ann Biomed Eng 38, 1977–1988 (2010). https://doi.org/10.1007/s10439-010-0035-1

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