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Optimization of lactic acid production by immobilized Lactococcus lactis IO-1

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Production of lactic acid from glucose by immobilized cells of Lactococcus lactis IO-1 was investigated using cells that had been immobilized by either entrapment in beads of alginate or encapsulation in microcapsules of alginate membrane. The fermentation process was optimized in shake flasks using the Taguchi method and then further assessed in a production bioreactor. The bioreactor consisted of a packed bed of immobilized cells and its operation involved recycling of the broth through the bed. Both batch and continuous modes of operation of the reactor were investigated. Microencapsulation proved to be the better method of immobilization. For microencapsulated cells at immobilized cell concentration of 5.3 g l−1, the optimal production medium had the following initial concentrations of nutrients (g l−1): glucose 45, yeast extract 10, beef extract 10, peptone 7.5 and calcium chloride 10 at an initial pH of 6.85. Under these conditions, at 37 °C, the volumetric productivity of lactic acid in shake flasks was 1.8 g l−1 h−1. Use of a packed bed of encapsulated cells with recycle of the broth through the bed, increased the volumetric productivity to 4.5 g l−1 h−1. The packed bed could be used in repeated batch runs to produce lactic acid.

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Acknowledgments

This work was financially supported by the Graduate School, Kasetsart University, Thailand, in fiscal year 2004 and officially promoted by Pingtung University, Taiwan, under the student exchange program with Kasetsart University in fiscal year 2005.

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Authors and Affiliations

  1. Department of Biotechnology, Kasetsart University, Bangkok, 10900, Thailand

    Sarote Sirisansaneeyakul, Tiyaporn Luangpipat & Wirat Vanichsriratana

  2. Department of Chemical Engineering, Kasetsart University, Bangkok, 10900, Thailand

    Thongchai Srinophakun

  3. Department of Food Science, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan, ROC

    Henry Ho-Hsien Chen

  4. Institute of Technology and Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand

    Yusuf Chisti

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  1. Sarote Sirisansaneeyakul
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  2. Tiyaporn Luangpipat
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Correspondence to Sarote Sirisansaneeyakul.

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Sirisansaneeyakul, S., Luangpipat, T., Vanichsriratana, W. et al. Optimization of lactic acid production by immobilized Lactococcus lactis IO-1. J Ind Microbiol Biotechnol 34, 381–391 (2007). https://doi.org/10.1007/s10295-007-0208-6

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  • DOI: https://doi.org/10.1007/s10295-007-0208-6

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