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Survival of anaerobic fungus Caecomyces sp. in various preservation methods: a comparative study

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Mycoscience

Abstract

The present investigation was designed to observe the survival of the anaerobic fungus Caecomyces sp. in various routine preservation methods. Among all the treatments, cryopreservation of fungi at −70 °C with glycerol was found to be most effective for long-term maintenance (more than 90 days) of rumen fungi, followed by dimethyl sulfoxide (DMSO) and ethylene glycol (up to 60 days). In contrast, at −196 °C, DMSO showed maximum survival (more than 90 days), followed by glycerol (up to 90 days) and ethylene glycol (up to 30 days). At 39 °C, maximum survival (up to 30 days) was observed with soft agar and wheat straw; at refrigeration temperature, preservation with Orpin’s media containing straw showed maximum survival (up to 30 days).

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Acknowledgments

The authors are thankful for the research fellowships and funding provided by National Dairy Research Institute, Karnal, and Indian Council of Agricultural Research, New Delhi, India.

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Authors and Affiliations

  1. Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, Haryana, India

    Ravinder Nagpal, Anil Kumar Puniya & Kishan Singh

  2. Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal, 132001, Haryana, India

    Jatinder Paul Sehgal

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  1. Ravinder Nagpal
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  2. Anil Kumar Puniya
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  3. Jatinder Paul Sehgal
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  4. Kishan Singh
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Correspondence to Ravinder Nagpal.

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Nagpal, R., Puniya, A.K., Sehgal, J.P. et al. Survival of anaerobic fungus Caecomyces sp. in various preservation methods: a comparative study. Mycoscience 53, 427–432 (2012). https://doi.org/10.1007/s10267-012-0187-y

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  • DOI: https://doi.org/10.1007/s10267-012-0187-y

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