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Components of the gene network associated with genotype-dependent response of wheat to the Fusarium mycotoxin deoxynivalenol

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Abstract

The Fusarium mycotoxin deoxynivalenol (DON) facilitates fungal spread within wheat tissue and the development of Fusarium head blight disease. The ability of wheat spikelets to resist DON-induced bleaching is genotype-dependent. In wheat cultivar (cv.) CM82036 DON resistance is associated with a quantitative trait locus, Fhb1, located on the short arm of chromosome 3B. Gene expression profiling (microarray and real-time RT-PCR analyses) of DON-treated spikelets of progeny derived from a cross between cv. CM82036 and the DON-susceptible cv. Remus discriminated ten toxin-responsive transcripts associated with the inheritance of DON resistance and Fhb1. These genes do not exclusively map to Fhb1. Based on the putative function of the ten Fhb1-associated transcripts, we discuss how cascades involving classical metabolite biotransformation and sequestration processes, alleviation of oxidative stress and promotion of cell survival might contribute to the host response and defence against DON.

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Acknowledgements

We thank Fany Doustaly for technical assistance. We thank Dr. Hermann Buerstmayr (IFA-Tulln, Austria) and the Wheat Genetics Resource Center at Kansas State University (Manhattan, Kansas, USA) for providing wheat seed. We also thank Dr. Peader O’ Gaora (Conway Institute, University College Dublin, Ireland) for critical reading of the microarray analyses section.

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Author notes

  1. Khairul I. Ansari

    Present address: Department of Chemistry and Biochemistry, University of Texas at Arlington, 700 Planetarium Place, Arlington, TX, 76019, USA

  2. Josephine M. Brennan

    Present address: Plant Health Laboratory, Department of Agriculture and Food, Backweston, Co. Kildare, Ireland

  3. Xuejun Hu

    Present address: Medical College, Dalian University, Dalian, 116622, China

  4. Stephanie Walter

    Present address: Department of Integrated Pest Management, Research Centre Flakkebjerg, University of Aarhus, Forsøgsvej 1, 4200, Slagelse, Denmark

Authors and Affiliations

  1. Molecular Plant-Microbe Interactions Laboratory, School of Biology and Environmental Sciences, College of Life Sciences, University College Dublin, Belfield, Dublin 4, Ireland

    Stephanie Walter, Josephine M. Brennan, Chanemougasoundharam Arunachalam, Khairul I. Ansari, Xuejun Hu, Mojibur R. Khan, Gerald Leonard, Damian Egan & Fiona M. Doohan

  2. Biogenetics-Natural Resources Division, Bioresources Department, Austrian Research Centers GmbH (ARC), 2444 Seibersdorf, Donau City Str. 1, 1220, Vienna, Austria

    Friederike Trognitz & Bodo Trognitz

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Correspondence to Stephanie Walter.

Additional information

This research was funded by Science Foundation Ireland (project 03-IN3-B414) and EU FP5 project FUCOMYR (QLRT-2000-02044).

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Walter, S., Brennan, J.M., Arunachalam, C. et al. Components of the gene network associated with genotype-dependent response of wheat to the Fusarium mycotoxin deoxynivalenol. Funct Integr Genomics 8, 421–427 (2008). https://doi.org/10.1007/s10142-008-0089-4

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  • DOI: https://doi.org/10.1007/s10142-008-0089-4

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