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New quantitative trait loci that regulate wound healing in an intercross progeny from DBA/1J and 129×1/SvJ inbred strains of mice

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Abstract

Wound healing/regeneration mouse models are few, and studies performed have mainly utilized crosses between MRL/MPJ (a good healer) and SJL/J (a poor healer) or MRL/lpr (a good healer) and C57BL/6J (a poor healer). Wound healing is a complex trait with many genes involved in the expression of the phenotype. Based on data from previous studies that common and additional quantitative trait loci (QTL) were identified using different crosses of inbred strains of mice for various complex traits, we hypothesized that a new cross would identify common and additional QTL, unique modes of inheritance, and interacting loci, which are responsible for variation in susceptibility to fast wound healing. In this study, we crossed DBA/1J (DBA, a good healer) and 129/SvJ (129, a poor healer) and performed a genome-wide scan using 492 (DBA×129) F2 mice and 98 markers to identify QTL that regulate wound healing/regeneration. Four QTL on chromosomes 1, 4, 12, and 18 were identified which contributed toward wound healing in F2 mice and accounted for 17.1% of the phenotypic variation in ear punch healing. Surprisingly, locus interactions contributed to 55.7% of the phenotype variation in ear punch healing. In conclusion, we have identified novel QTL and shown that minor interacting loci contribute significantly to wound healing in DBA×129 mice cross.

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Acknowledgements

Assistance award #DAMDBA7-99-1-9571 supported this work. The US Army Medical Research Acquisition Activity, 820 Chandler Street, Fort Detrick, MD 21702-5014, USA, is the awarding and administering acquisition office. The information contained in this publication does not necessarily reflect the position or policy of the Government, and no official endorsement should be inferred. The authors wish to thank Heather Davidson and Melanie Hamilton-Ulland for their excellent technical support and the JL Pettis VA Medical Center for their support.

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Authors and Affiliations

  1. Musculoskeletal Disease Center (151), Jerry L. Pettis Memorial Veterans Administration Medical Center, 11201 Benton Street, Loma Linda, CA, 92357, USA

    Godfred L. Masinde, Runzhi Li, Bay Nguyen, Hongrun Yu, Apurva K. Srivastava, Bouchra Edderkaoui, Jon E. Wergedal, David J. Baylink & Subburaman Mohan

  2. Department of Medicine, Loma Linda University, Loma Linda, CA, 92350, USA

    Apurva K. Srivastava, Jon E. Wergedal, David J. Baylink & Subburaman Mohan

  3. Department of Biochemistry, Loma Linda University, Loma Linda, CA, 92350, USA

    Subburaman Mohan

  4. Department of Physiology, Loma Linda University, Loma Linda, CA, 92350, USA

    Subburaman Mohan

Authors
  1. Godfred L. Masinde
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  2. Runzhi Li
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  3. Bay Nguyen
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  4. Hongrun Yu
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  5. Apurva K. Srivastava
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  6. Bouchra Edderkaoui
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  7. Jon E. Wergedal
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  8. David J. Baylink
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  9. Subburaman Mohan
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Corresponding author

Correspondence to Subburaman Mohan.

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The authors Masinde, Li, and Nguyen contributed equally to this article.

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Masinde, G.L., Li, R., Nguyen, B. et al. New quantitative trait loci that regulate wound healing in an intercross progeny from DBA/1J and 129×1/SvJ inbred strains of mice. Funct Integr Genomics 6, 157–163 (2006). https://doi.org/10.1007/s10142-005-0004-1

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  • DOI: https://doi.org/10.1007/s10142-005-0004-1

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