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Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio

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Abstract

Microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by WSSV and heat-inactivated Vibrio anguillarum, respectively. At 6 h post challenge (HPC), a total of 806 clones showed differential expression profile in WSSV-challenged samples, but not in Vibrio-challenged samples. The genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post WSSV-challenged (HPC-WSSV) tissues. However, a total of 155 clones showed differential expression in the Vibrio-challenged samples, but not in WSSV-challenged samples. Serine-type endopeptidase and lysosome-related genes were the most upregulated elements in tissues 6 h post Vibrio challenge (HPC-Vibrio). Totally, 188 clones showed differential expression in both 6 and 12 HPC-WSSV and HPC-Vibrio samples. Most of the differentially expressed genes (185/188) were downregulated in the samples of 12 HPC-WSSV, whereas upregulated in the samples at 6 and 12 HPC-Vibrio and 6 HPC-WSSV. The expression profiles of three differentially expressed genes identified in microarray hybridization were analyzed in hemocytes, lymphoid organ, and hepatopancreas of shrimp challenged by WSSV or Vibrio through real-time PCR. The results further confirmed the microarray hybridization results. The data will provide great help for us in understanding the immune mechanism of shrimp responding to WSSV or Vibrio.

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Acknowledgements

This work is financially supported by the Major State Basic Research Development Program of China (973 program) 2006CB101804, National Natural Science Foundation of China (30771639), and National 863 High-tech Program of China (2007AA09Z430, 2006AA10A402).

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, People’s Republic of China

    Bing Wang, Fuhua Li, Wei Luan, Yusu Xie, Chengsong Zhang, Zhan Luo, Lang Gui, Hui Yan & Jianhai Xiang

  2. Graduate School, Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China

    Wei Luan, Yusu Xie, Chengsong Zhang, Zhan Luo, Lang Gui & Hui Yan

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  1. Bing Wang
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  2. Fuhua Li
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  3. Wei Luan
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Correspondence to Jianhai Xiang.

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Wang and Li contributed equally to this work.

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Wang, B., Li, F., Luan, W. et al. Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio . Mar Biotechnol 10, 664–675 (2008). https://doi.org/10.1007/s10126-008-9105-x

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  • DOI: https://doi.org/10.1007/s10126-008-9105-x

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