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Effect of photobiomodulation on expression of IL-1β in skeletal muscle following acute injury

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Abstract

Muscle repair is regulated by growth factors and cytokines. Low-level laser therapy (LLLT) seems to influence acute inflammation and accelerate skeletal muscle repair. This study verifies the effect of LLLT on the expression of IL-1β in the tibialis anterior (TA) muscle of rats following acute injury. Wistar rats (n = 35) were allocated into three groups: control (without lesion and LLLT, n = 5), injury group (n = 15), and injury + LLLT group (n = 15). The acute injury was induced by the contact with a cooled metal probe (3 mm in diameter) during 10 s, twice, in the same muscle area. LLLT was used three times a week using the InGaAlP laser (660 nm; beam spot of 0.04 cm2, output power of 20 mW, power density of 500 mW/cm2, and energy density of 5 J/cm2 during 10 s). The animals were analyzed at 1, 7, and 14 days following injury. TA muscles samples were used for obtaining total RNA and performing cDNA synthesis. Real-time polymerase chain reactions were realized using IL-1β primer. There was a decrease in IL-1β expression after 7 days in LLLT group in comparison with the no treated group. In conclusion, LLLT was able to decrease IL-1β expression during the skeletal muscle repair following an acute injury.

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Acknowledgments

The authors would like to thank UNINOVE and FAPESP (2011/17638-2; 2011/04452-8) for financial support.

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Authors and Affiliations

  1. Rehabilitation Sciences and Biophotonics, Universidade Nove de Julho—UNINOVE, São Paulo, São Paulo, Brazil

    Kristianne Porta Santos Fernandes & Raquel Agnelli Mesquita Ferrari

  2. Department of Oral Pathology, School of Dentistry, Universidade de São Paulo, São Paulo, Brazil

    Fábio Daumas Nunes

  3. Rehabilitation Sciences, Universidade Nove de Julho—UNINOVE, São Paulo, São Paulo, Brazil

    Agnelo Neves Alves, Nadhia Helena Costa Souza, José Antônio Silva Jr & Sandra Kalil Bussadori

  4. Departamento de Pós Graduação, Mestrado em Ciências da Reabilitação, Universidade Nove de Julho—UNINOVE, Av. Francisco Matarazzo, 612, Água Branca, CEP 05001-100, São Paulo, São Paulo, Brazil

    Kristianne Porta Santos Fernandes

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  1. Kristianne Porta Santos Fernandes
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  2. Agnelo Neves Alves
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  3. Fábio Daumas Nunes
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  4. Nadhia Helena Costa Souza
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  5. José Antônio Silva Jr
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  6. Sandra Kalil Bussadori
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  7. Raquel Agnelli Mesquita Ferrari
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Correspondence to Kristianne Porta Santos Fernandes.

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Fernandes, K.P.S., Alves, A.N., Nunes, F.D. et al. Effect of photobiomodulation on expression of IL-1β in skeletal muscle following acute injury. Lasers Med Sci 28, 1043–1046 (2013). https://doi.org/10.1007/s10103-012-1233-x

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  • DOI: https://doi.org/10.1007/s10103-012-1233-x

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