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Oral administration of inosine promotes recovery after experimental spinal cord injury in rat

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Abstract

Inosine, a purine nucleoside, is one of the novel substances, which can preserve the neuronal and glial viability and stimulate intact neurons to extend axons. We, herein, evaluated the effect of oral inosine treatment on spinal cord injury (SCI) recovery by means of locomotor and bladder function, quantification of neurons and spinal cord tissue sparing. Rats after compression SCI were divided into groups—SCI-Aqua and SCI-Inosine (daily application of aqua for injection or inosine)—locomotion of hind limbs (BBB score) and urinary bladder function were evaluated from day 1 to 28 after SCI. The neuronal profile was determined by immunohistochemistry with NeuN antibodies and tissue sparing by Luxol fast blue staining method. SCI affected the functional movement of hind limbs in both groups with gradual improvement (increased BBB score) during survival. However, we found a significant difference in BBB score and recovery of bladder function between SCI-Aqua and SCI-Inosine groups during the second week of survival following SCI. In addition, the number of NeuN positive cells and percentage of tissue sparing was also significantly higher in SCI-Inosine group when compared with the SCI-Aqua group. Daily oral administration of inosine after SCI throughout the survival was beneficial for locomotion and micturition, neuronal survival and tissue sparing. This indicates that inosine may represent one of the co-stimulatory factors for treatment strategies to promote neuronal plasticity after SCI.

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Acknowledgments

This work was supported by the financial support from the Scientific Grant Agency of Ministry of Education, Science, Research and Sport of the Slovak Republic and the Slovak academy of Sciences (VEGA no. 1/2945/12, VEGA no. 2/0169/13) and the Slovak Research and Development Agency (APVV no. 0472/11).

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Authors and Affiliations

  1. Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81, Kosice, Slovakia

    Maria Kuricova, Valent Ledecky, Tomas Liptak & Aladar Madari

  2. Institute of Neurobiology, Slovak Academy of Sciences, Centre of Excellence for Brain Research, Soltesovej 4-6, 040 01, Kosice, Slovakia

    Ivana Grulova, Lucia Slovinska, Miriam Nagyova & Dasa Cizkova

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  1. Maria Kuricova
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  2. Valent Ledecky
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Correspondence to Maria Kuricova.

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Kuricova, M., Ledecky, V., Liptak, T. et al. Oral administration of inosine promotes recovery after experimental spinal cord injury in rat. Neurol Sci 35, 1785–1791 (2014). https://doi.org/10.1007/s10072-014-1840-3

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  • DOI: https://doi.org/10.1007/s10072-014-1840-3

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