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Mechanistic insight into the initiation step of the reaction of Burkholderia pseudomallei catalase-peroxidase with peroxyacetic acid

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Abstract

The reaction of the catalase-peroxidase of Burkholderia pseudomallei with peroxyacetic acid has been analyzed using stopped-flow spectrophotometry. Two well-defined species were observed, the first defined by an increase in intensity and narrowing of the Soret band at 407 nm and a 10-nm shift of the charge transfer band from 635 to 625 nm. These features are consistent with a ferric spectrum with a greater proportion of sixth-coordination character and are assigned to an FeIII–peroxyacetic acid complex. Complementary 9-GHz EPR characterization of the changes in the ferric signal of the resting enzyme induced by the binding of acetate in the heme pocket substantiates the proposal. Kinetic analysis of the spectral changes as a function of peroxyacetic acid concentration revealed two independent peroxyacetic acid binding events, one coincident with formation of the FeIII–peroxyacetic acid complex and the other coincident with the heme oxidation to the subsequent ferryl intermediate. A model to explain the need for two peroxyacetic acid binding events is proposed. The reaction of the W330F variant followed similar kinetics, although the characteristic spectral features of the FeIV=O Por•+ species were detected. The variant D141A lacking an aspartate at the entrance to the heme cavity as well as the R108A and D141A/R108A variants showed no evidence for the FeIII–peroxyacetic acid complex, only the formation of ferryl species with absorbance maxima at 414, 545, and 585 nm.

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Acknowledgments

This work was supported by the French CNRS and CEA Saclay (to A.I.), a Ph.D. Fellowship (CFR contract from CEA Saclay to J.C.), and grants from the Natural Sciences and Engineering Research Council of Canada (to P.C.L.) and the Canadian Research Chair Program (to P.C.L.).

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Authors and Affiliations

  1. Department of Microbiology, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

    Ben Wiseman & Peter C. Loewen

  2. Centre d’Etudes de Saclay, IBITEC, Service de Bioénergétique, Biologie Structurale et Mécanismes, CNRS URA 2096, Bat 532, 91191, Gif-sur-Yvette, France

    Julie Colin & Anabella Ivancich

  3. Department of Chemistry and Biochemistry, School of Life Sciences, University of Sussex, Brighton, BN1 9QG, UK

    Andrew T. Smith

Authors
  1. Ben Wiseman
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  2. Julie Colin
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  3. Andrew T. Smith
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  4. Anabella Ivancich
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  5. Peter C. Loewen
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Corresponding authors

Correspondence to Anabella Ivancich or Peter C. Loewen.

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Wiseman, B., Colin, J., Smith, A.T. et al. Mechanistic insight into the initiation step of the reaction of Burkholderia pseudomallei catalase-peroxidase with peroxyacetic acid. J Biol Inorg Chem 14, 801–811 (2009). https://doi.org/10.1007/s00775-009-0493-9

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  • DOI: https://doi.org/10.1007/s00775-009-0493-9

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