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Resonance Raman spectroscopy of cytochrome c peroxidase variants that mimic manganese peroxidase

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Abstract

Cytochrome c peroxidase (CcP) variants with an engineered Mn(II) binding site, including MnCcP [CcP(MI, G41E, V45E, H181D)], MnCcP(W191F), and MnCcP(W191F, W51F), that mimic manganese peroxidase (MnP), have been characterized by resonance Raman (RR) spectroscopy. Analysis of the Raman bands in the 200–700 cm−1 and 1300–1650 cm−1 regions indicates that both the coordination and spin state of the heme iron in the variants differ from that of CcP(MI), the recombinant yeast CcP containing additional Met-Ile residues at the N-terminus. At neutral pH the frequencies of the ν3 mode indicate that a pure five-coordinate heme iron exists in CcP(MI) whereas a six-coordinate low-spin iron is the dominant species in the CcP variants with the engineered Mn(II) binding site. The H181D mutation, which weakens the proximal linkage to the heme iron, may be responsible for these spectral and structural changes. Raman spectra of the variants CcP(MI, W191F) and CcP(MI, W191F, W51F) were also obtained to clarify the structural and functional roles of mutations at two tryptophan sites. The W51F mutation was found to disrupt H-bonding to the distal water molecules and the resulting variants tended to form transitional or mixed coordination states that possess spectral and structural features similar to that of MnP. Such structural features, with a loosened distal water, may facilitate the binding of H2O2 and increase the rate constant for compound I formation. This effect, in addition to the elimination of an H-bond to ferryl oxygen by the same mutation, accounts for the increased MnP specific activity of MnCcP(W191F, W51F).

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Abbreviations

CcP:

cytochrome c peroxidase

CcP(MI):

recombinant yeast CcP containing Met-Ile at the N-terminus in addition to the normal wild-type CcP sequence

HRP:

horseradish peroxidase

MnCcP:

CcP(MI, G41E, V45E, H181D)

MnCcP(W191F):

CcP(MI, G41E, V45E, H181D, W191F)

MnCcP(W191F, W51F):

CcP(MI, G41E, V45E, H181D, W191F, W51F)

MnP:

manganese peroxidase

RR:

resonance Raman

WtCcP:

wild-type cytochrome c peroxidase

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Acknowledgements

This work was supported by the National Institutes of Health (grants S06GM08047 and GM62211).

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Authors and Affiliations

  1. Department of Chemistry, Jackson State University, Box 17910, Jackson, MS , 39217-0510, USA

    Manliang Feng, Hiroyasu Tachikawa & Xiaotang Wang

  2. Departments of Chemistry and Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL , 61801, USA

    Thomas D. Pfister, Alan J. Gengenbach & Yi Lu

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  1. Manliang Feng
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  2. Hiroyasu Tachikawa
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  3. Xiaotang Wang
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  4. Thomas D. Pfister
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  5. Alan J. Gengenbach
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  6. Yi Lu
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Correspondence to Hiroyasu Tachikawa.

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Feng, M., Tachikawa, H., Wang, X. et al. Resonance Raman spectroscopy of cytochrome c peroxidase variants that mimic manganese peroxidase. J Biol Inorg Chem 8, 699–706 (2003). https://doi.org/10.1007/s00775-003-0460-9

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