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PCAF acetylates Runx2 and promotes osteoblast differentiation

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Abstract

Osteoblasts play a crucial role in bone formation. However, the molecular mechanisms involved in osteoblast differentiation remain largely unclear. Runt-related gene 2 (Runx2) is a master transcriptional factor for osteoblast differentiation. Here we reported that p300/CBP-associated factor (PCAF) directly binds to Runx2 and acetylates Runx2, leading to an increase in its transcriptional activity. Upregulation of PCAF in MC3T3-E1 cells increases the expression of osteogenic marker genes including alkaline phosphatase (ALP), osteocalcin (Ocn), and Osteopontin (Opn), and ALP activity was stimulated as well. Consequently, the mineralization of MC3T3-E1 cells was remarkably improved by PCAF. In contrast, PCAF knockdown decreases the mRNA levels of ALP, Ocn, and Opn. ALP activity and the mineralized area were attenuated under PCAF knockdown conditions. These results indicate that PCAF is an important regulator for promoting osteoblast differentiation via acetylation modification of Runx2.

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Acknowledgments

This work was supported in part by The Science and Technology Development Program of Nanjing (2012sc311020).

Conflict of interest

The authors have no conflicts of interest.

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Authors and Affiliations

  1. Department of Orthopaedics, 98 Hospital of PLA, Zhejiang, 313000, Huzhou, China

    Chao-Yang Wang, Zhong Wang, Jun-Ming Tan, Shun-Min Xing & De-Chun Chen

  2. Department of Orthopaedics, 81 Hospital of PLA, Nanjing, 210002, Jiangsu, China

    Shu-Feng Yang

  3. Department of Orthopaedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China

    Sheng-Ming Xu & Wen Yuan

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  1. Chao-Yang Wang
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  2. Shu-Feng Yang
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  3. Zhong Wang
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Corresponding authors

Correspondence to Chao-Yang Wang or Wen Yuan.

Additional information

C.-Y. Wang and S.-F. Yang contributed equally to this work.

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Wang, CY., Yang, SF., Wang, Z. et al. PCAF acetylates Runx2 and promotes osteoblast differentiation. J Bone Miner Metab 31, 381–389 (2013). https://doi.org/10.1007/s00774-013-0428-y

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  • DOI: https://doi.org/10.1007/s00774-013-0428-y

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