Abstract
Molecular dynamics simulations were carried out for the mutant oseltamivir-NA complex, to provide detailed information on the oseltamivir-resistance resulting from the H274Y mutation in neuraminidase (NA) of avian influenza H5N1 viruses. In contrast with a previous proposal, the H274Y mutation does not prevent E276 and R224 from forming the hydrophobic pocket for the oseltamivir bulky group. Instead, reduction of the hydrophobicity and size of pocket in the area around an ethyl moiety at this bulky group were found to be the source of the oseltamivir-resistance. These changes were primarily due to the dramatic rotation of the hydrophilic –COO− group of E276 toward the ethyl moiety. In addition, hydrogen-bonding interactions with N1 residues at the -NH3 + and -NHAc groups of oseltamivir were replaced by a water molecule. The calculated binding affinity of oseltamivir to NA was significantly reduced from −14.6 kcal mol−1 in the wild-type to −9.9 kcal mol−1 in the mutant-type.
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Acknowledgments
This work was supported by the Thailand Research Fund and the Royal Golden Jubilee Ph.D. Program (3.C.CU/48/F.1). T. R. thanks the Commission on Higher Education for the Post-Doctoral Program. The authors thank the Computational Chemistry Unit Cell, Chulalongkorn University and National Center for Genetic Engineering and Biotechnology (BIOTEC) for computing facilities. The National Nanotechnology Center (NANOTEC) supplied Discovery Studio for molecular graphics.
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M. Malaisree and T. Rungrotmongkol have contributed equally to this work.
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Malaisree, M., Rungrotmongkol, T., Nunthaboot, N. et al. Source of oseltamivir resistance in avian influenza H5N1 virus with the H274Y mutation. Amino Acids 37, 725–732 (2009). https://doi.org/10.1007/s00726-008-0201-z
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DOI: https://doi.org/10.1007/s00726-008-0201-z