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Effect of heat shock on ultrastructure and calcium distribution in Lavandula pinnata L. glandular trichomes

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Abstract

The effects of heat shock (HS) on the ultrastructure and calcium distribution of Lavandula pinnata secretory trichomes are examined using transmission electron microscopy and potassium antimonate precipitation. After 48-h HS at 40°C, plastids become distorted and lack stroma and osmiophilic deposits, the cristae of the mitochondria become indistinct, the endoplasmic reticulum acquires a chain-like appearance with ribosomes prominently attached to the lamellae, and the plasma and organelle membranes become distorted. Heat shock is associated with a decrease in calcium precipitates in the trichomes, while the number of precipitates increases in the mesophyll cells. Prolonged exposure to elevated calcium levels may be toxic to the mesophyll cells, while the lack of calcium in the glands cell may deprive them of the normal protective advantages of elevated calcium levels. The inequality in calcium distribution may result not only from uptake from the transpiration stream, but also from redistribution of calcium from the trichomes to the mesophyll cells.

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Acknowledgments

The first author thanks Xu, XingLan and Hu, XiaoYing, instructors in the Electron Microscopy facility of the South China Botanical Garden, for their assistance in learning EM. We also thank two anonymous reviewers for their helpful comments on the manuscript.

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The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Guangdong Research Institute of Traditional Chinese Medicine, Guangzhou, 510520, China

    S. S. Huang

  2. Department of Biology, University of North Carolina at Greensboro, 312 Eberhart, P. O. Box 26170, Greensboro, NC, 27402-6170, USA

    B. K. Kirchoff

  3. Key Laboratory of Digital Botanical Garden in Guangdong, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    J. P. Liao

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  1. S. S. Huang
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Correspondence to S. S. Huang.

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Huang, S.S., Kirchoff, B.K. & Liao, J.P. Effect of heat shock on ultrastructure and calcium distribution in Lavandula pinnata L. glandular trichomes. Protoplasma 250, 185–196 (2013). https://doi.org/10.1007/s00709-012-0393-7

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  • DOI: https://doi.org/10.1007/s00709-012-0393-7

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