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Antiherpetic potential of 6-bromoindirubin-3′-acetoxime (BIO-acetoxime) in human oral epithelial cells

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Abstract

Glycogen synthase kinase 3 (GSK-3) functions in the regulation of glycogen metabolism, in the cell cycle, and in immune responses and is targeted by some viruses to favor the viral life cycle. Inhibition of GSK-3 by 6-bromoindirubin-3′-acetoxime (BIO-acetoxime), a synthetic derivative of a compound from the Mediterranean mollusk Hexaplex trunculus, protects cells from varicella infection. In this study, we examined the effects of BIO-acetoxime against herpes simplex virus-1 (HSV-1) infection in human oral epithelial cells, which represent a natural target cell type. The results revealed that BIO-acetoxime relieves HSV-1-induced cytopathic effects and apoptosis. We also found that BIO-acetoxime reduced viral yields and the expression of different classes of viral proteins. Furthermore, addition of BIO-acetoxime before, simultaneously with or after HSV-1 infection significantly reduced viral yields. Collectively, BIO-acetoxime may suppress viral gene expression and protect oral epithelial cells from HSV-1 infection. These results suggest the possible involvement of GSK-3 in HSV-1 infection.

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Acknowledgements

This investigation was supported by the grant from Ministry of Education, Aim for the Top University Plan and by the Research Grant NSC 101-2314-B-010-033-MY2 from the National Science Council, Taiwan. The authors acknowledge the technical support provided by the Imaging Core Facility of Nanotechnology of the University System of Taiwan-National Yang-Ming University (UST-YMU), especially Pei-Jun Chen and Shu-Fen Lin for their technical support.

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Authors and Affiliations

  1. Institute of Oral Biology, National Yang-Ming University, No. 155, Sec. 2, Li-Nong St., Pei-Tou, Taipei, 11221, Taiwan

    Mei-Ju Hsu & Shan-Ling Hung

  2. Department of Dentistry, National Yang-Ming University, No. 155, Sec. 2, Li-Nong St., Pei-Tou, Taipei, 11221, Taiwan

    Shan-Ling Hung

  3. Department of Stomatology, Taipei Veterans General Hospital, No. 201, Sec. 2, Shih-Pai Road, Taipei, 11217, Taiwan

    Shan-Ling Hung

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  1. Mei-Ju Hsu
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Correspondence to Shan-Ling Hung.

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Hsu, MJ., Hung, SL. Antiherpetic potential of 6-bromoindirubin-3′-acetoxime (BIO-acetoxime) in human oral epithelial cells. Arch Virol 158, 1287–1296 (2013). https://doi.org/10.1007/s00705-013-1629-3

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  • DOI: https://doi.org/10.1007/s00705-013-1629-3

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