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Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control

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Abstract

The recent and continuing HFMD outbreak caused by EV71 in several provinces of China since March 2008 has affected thousands of children and resulted in nearly 50 deaths. In this study, a sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV71 and CV-A16. By using an internal amplification control, the real-time assay achieves detection of samples containing inhibitors and avoids false negatives. It should prove useful for clinical diagnosis of EV71 or CV-A16 infections.

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Acknowledgments

The work was partially supported by the National Natural Science Foundation of China (No. 20676042) and a grant from Shenzhen Taitai genomics, Inc.

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Authors and Affiliations

  1. Department of Food, Institution of Food safety, College of Light Industry and Food, South China University of Technology, 510640, Guangzhou, China

    Xing-Long Xiao, Yi-Gang Yu, Gu Chen, Dong-Mei Liu, Xiao-Feng Li, Xiao-Quan Yang & Hui Wu

  2. Shenzhen Taitai Genomics, Inc., 518057, Shenzhen, China

    Xing-Long Xiao, Hui-Fang Li & Jing-Wei Zhang

  3. Shenzhen Center for Disease Control and Prevention, 518000, Shenzhen, China

    Ya-Qing He & Hong Yang

Authors
  1. Xing-Long Xiao
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  2. Ya-Qing He
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  3. Yi-Gang Yu
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  4. Hong Yang
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  5. Gu Chen
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  6. Hui-Fang Li
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  7. Jing-Wei Zhang
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  8. Dong-Mei Liu
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  9. Xiao-Feng Li
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  10. Xiao-Quan Yang
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  11. Hui Wu
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Corresponding author

Correspondence to Hui Wu.

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Xiao, XL., He, YQ., Yu, YG. et al. Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control. Arch Virol 154, 121–125 (2009). https://doi.org/10.1007/s00705-008-0266-8

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  • DOI: https://doi.org/10.1007/s00705-008-0266-8

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