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Genetic variability of group A and B human respiratory syncytial viruses isolated from 3 provinces in China

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Summary

The genetic variability of HRSV in China was studied using nucleotide sequencing of the hypervariable C-terminal region of the G protein gene and phylogenetic analysis on 80 isolates obtained from three children’s hospitals over a period of three epidemic seasons, 1990/1991, 2000/2001, and 2003/2004. The results showed that 76/80 of these isolates belonged to group A and 4/80 belonged to group B. Phylogenetic analysis revealed that most of the group A isolates were genotype GA2 (74/76 isolates), and the other two isolates were GA3 and GA5. All group B isolates clustered into genotype GB3. There was substantial variation among the GA2 isolates, with nucleotide sequence and amino acid homologies ranging from 88.1–100% and 78.4–100%, respectively, in the hypervariable C-terminal region of the G protein gene. One group B virus, HRSV/Beijing/B/04/11, contained a 60-nucleotide duplication in the C-terminal region of the G protein, which was similar to what has been reported previously for isolates in several countries. This is the first report on the genetic diversity of human respiratory syncytial virus isolated during epidemic periods from children in China. These data provided a preliminary evaluation of patterns of circulation and the genetic diversity of isolates associated with HRSV epidemics within China.

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Abbreviations

ALRI:

Acute lower respiratory tract infection

CCDC:

Centers for Disease Control and Prevention of China

HRSV:

Human respiratory syncytial virus

PCR:

Polymerase chain reaction

RT-PCR:

Reverse transcription-polymerase chain reaction

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Authors and Affiliations

  1. China Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Beijing, China

    Y. Zhang, W. Xu & G. Liang

  2. Beijing Children’s Hospital, Beijing, China

    K. Shen, Z. Xie, Q. Lu & C. Liu

  3. Changchun Children’s Hospital, Changchun, China

    L. Sun

  4. Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, FDA, Bethesda, MD, USA

    J. A. Beeler

  5. Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA

    L. J. Anderson

Authors
  1. Y. Zhang
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  2. W. Xu
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  3. K. Shen
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  4. Z. Xie
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  5. L. Sun
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  6. Q. Lu
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  7. C. Liu
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  8. G. Liang
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  9. J. A. Beeler
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  10. L. J. Anderson
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Zhang, Y., Xu, W., Shen, K. et al. Genetic variability of group A and B human respiratory syncytial viruses isolated from 3 provinces in China. Arch Virol 152, 1425–1434 (2007). https://doi.org/10.1007/s00705-007-0984-3

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  • DOI: https://doi.org/10.1007/s00705-007-0984-3

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