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Protein-directed in situ synthesis of platinum nanoparticles with superior peroxidase-like activity, and their use for photometric determination of hydrogen peroxide

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Abstract

Platinum nanoparticles (Pt-NPs) with sizes in the range from 10 to 30 nm were synthesized using protein-directed one-pot reduction. The model globular protein bovine serum albumin (BSA) was exploited as the template, and the resulting BSA/Pt-NPs were studied by transmission electron microscopy, energy dispersive X-ray spectroscopy, and resonance Rayleigh scattering spectroscopy. The modified nanoparticles display a peroxidase-like activity that was exploited in a rapid method for the colorimetric determination of hydrogen peroxide which can be detected in the 50 μM to 3 mM concentration range. The limit of detection is 7.9 μM, and the lowest concentration that can be visually detected is 200 μM.

Pt-NPs were synthesized using BSA-directed one-pot reduction and BSA/Pt-NPs composite can effectively catalyze the oxidation of TMB producing blue solution in the presence of H2O2.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No.21075078, 21105056) and the Natural Science Foundation of Shandong province, China (No.ZR2010BM005, ZR2011BQ001).

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Authors and Affiliations

  1. College of Chemistry and Material Science, Shandong Agricultural University, Taian, 271018, People’s Republic of China

    Lijian Chen, Nan Wang, Xindong Wang & Shiyun Ai

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  1. Lijian Chen
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  2. Nan Wang
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  3. Xindong Wang
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  4. Shiyun Ai
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Correspondence to Shiyun Ai.

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Chen, L., Wang, N., Wang, X. et al. Protein-directed in situ synthesis of platinum nanoparticles with superior peroxidase-like activity, and their use for photometric determination of hydrogen peroxide. Microchim Acta 180, 1517–1522 (2013). https://doi.org/10.1007/s00604-013-1068-6

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