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Tin disulfide nanoflakes decorated with gold nanoparticles for direct electrochemistry of glucose oxidase and glucose biosensing

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Abstract

Previously, we have prepared nanoflake-like tin disulfide (SnS2) and used for the immobilization of proteins and biosensing. We have now modified an electrode with a composite consisting of nanoflake-like SnS2 decorated with gold nanoparticles (Au-NPs) and have immobilized glucose oxidase (GOx) on its surface in order to study its direct electrochemistry. Scanning electron microscopy, electrochemical impedance spectroscopy, Fourier transform IR spectroscopy and cyclic voltammetry were used to examine the interaction between GOx and the AuNP-SnS2 film. It is shown that the composite film has a larger surface area and offers a microenvironment that facilitates the direct electron transfer between enzyme and electrode surface. The immobilized enzyme retains its bioactivity and undergoes a surface-controlled, reversible 2-proton and 2-electron transfer reaction, with an apparent electron transfer rate constant of 3.87 s -1. Compared to the nanoflake-like SnS2-based glucose sensor, the GOx-based biosensor exhibits a lower detection limit (1.0 :M), a better sensitivity (21.8 mA M -1  cm -2), and a wider linear range (from 0.02 to 1.3 mM). The sensor displays excellent selectivity, good reproducibility, and acceptable stability. It was successfully applied to reagentless sensing of glucose at −0.43 V.

The AuNPs decorated nanoflake-like SnS2 (AuNPs–SnS2) composite is for the first time prepared and used to construct novel glucose biosensor nanoflake-like SnS2 was firstly synthesized and SEM image of the nanoflake-like SnS2 (a) and TEM images of the nanoflake-like SnS2 (b), AuNPs (c) and AuNPs–SnS2 (d) are shown in above figure.

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Acknowledgments

This work was financially supported by National Natural Science Foundation of China (21075107, 21103148, 21005070 and 20975090), The Priority Academic Program Development of Jiangsu Higher Education Institution, Postdoctoral Science Foundation of China (20110491462), Postdoctoral Science Foundation of Jiangsu Province (1101020B, 1102142C) and the Open Research Fund of State Key Laboratory of Bioelectronics of Southeast University (2011E13).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, People’s Republic of China

    Juan Li, Zhanjun Yang, Yongcai Zhang, Suhua Yu, Qin Xu, Qishu Qu & Xiaoya Hu

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  1. Juan Li
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  2. Zhanjun Yang
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  3. Yongcai Zhang
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  4. Suhua Yu
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  5. Qin Xu
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  6. Qishu Qu
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  7. Xiaoya Hu
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Correspondence to Zhanjun Yang or Xiaoya Hu.

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Li, J., Yang, Z., Zhang, Y. et al. Tin disulfide nanoflakes decorated with gold nanoparticles for direct electrochemistry of glucose oxidase and glucose biosensing. Microchim Acta 179, 265–272 (2012). https://doi.org/10.1007/s00604-012-0889-z

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  • DOI: https://doi.org/10.1007/s00604-012-0889-z

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