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Surface activation of plasma-patterned carbon nanotube based DNA sensing electrodes

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Abstract

We have studied the effect of treatment of multiwalled carbon nanotubes (MWCNTs) for use in DNA-based biosensors with oxygen plasma. Well-patterned MWCNT electrodes were photolithographically fabricated on glass substrates. Pure oxygen was used for etching and functionalization of the MWCNT film in a lab-made plasma chamber. The resulting electrodes exhibited a dramatic change in the morphology of their surface, the chemical composition, and the electrochemical properties in terms of peak current and peak potential separation. The electrodes also showed increased DNA immobilization efficiency and much higher sensitivity in the detection of target DNA as compared to non-treated MWCNT electrodes. Plasma treatment was optimized and electrodes were characterized by atomic force microscopy, X-ray photoelectron spectroscopy, cyclic voltammetry, and differential pulse voltammetry.

Well-patterned MWCNT electrodes were photolithographically fabricated on glass substrates. Pure oxygen was used for etching and functionalization of the MWCNT film. The electrodes showed increased DNA immobilization efficiency and much higher sensitivity in the detection of target DNA as compared to non-treated MWCNT electrodes

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Acknowledgment

This work was supported by Grant No. K20601000002-07E0100-00210 from Korea Foundation for International Cooperation of Science & Technology.

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  1. Department of Biomicrosystem Technology, Korea University, Seoul, 136–701, Korea

    Eun Jin Park, Joon-Hyung Jin, Joon Hyub Kim & Nam Ki Min

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  1. Eun Jin Park
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  2. Joon-Hyung Jin
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  3. Joon Hyub Kim
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  4. Nam Ki Min
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Correspondence to Nam Ki Min.

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Park, E.J., Jin, JH., Kim, J.H. et al. Surface activation of plasma-patterned carbon nanotube based DNA sensing electrodes. Microchim Acta 174, 231–238 (2011). https://doi.org/10.1007/s00604-011-0605-4

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  • DOI: https://doi.org/10.1007/s00604-011-0605-4

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