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PNA-Modified Magnetic Nanoparticles and Their Hybridization with Single-Stranded DNA Target: Surface Enhanced Raman Scatterings Study

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Abstract.

A 4-pyridyldithiol-derivatized peptide nucleic acid (PNA), which was designed to recognize a specific gene, was attached to 3-mercapropropyloxysilane coated magnetic nanoparticles (MNPs) via a thiol-disulfide exchange reaction. Subsequently, PNA-functionalized magnetic nanoparticles (PMNPs) were prepared. Sequentially the PMNPs were challenged with non-complementary and perfect-match DNA targets. The entire procedure was monitored using surface-enhanced Raman scattering (SERS). The results showed that the PMNPs were able to efficiently hybridize with the perfect-match ssDNA target and showed no affinity towards non-complementary DNA. This approach may provide a means of direct and label-free gene analysis.

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Authors and Affiliations

  1. College of Life and Environment Science, Shanghai Normal University, 200234, Shanghai, China

    Feng Wang, Hebai Shen, Jie Feng & Haifeng Yang

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  1. Feng Wang
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  2. Hebai Shen
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  3. Jie Feng
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  4. Haifeng Yang
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Correspondence to Hebai Shen.

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Wang, F., Shen, H., Feng, J. et al. PNA-Modified Magnetic Nanoparticles and Their Hybridization with Single-Stranded DNA Target: Surface Enhanced Raman Scatterings Study. Microchim Acta 153, 15–20 (2006). https://doi.org/10.1007/s00604-005-0460-2

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  • DOI: https://doi.org/10.1007/s00604-005-0460-2

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