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Population genetic structure of an ectomycorrhizal fungus Amanita manginiana in a subtropical forest over two years

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Abstract

The population genetic structure of the late-stage fungus Amanita manginiana in a natural forest in Dujiangyan, southwest China was examined over two years using inter-simple sequence repeat (ISSR) markers. Seven ISSR primers were used and 170 bands were obtained in this population: 134/160 and 135/153 bands were polymorphic for sporocarps of 2001 and 2002, respectively. Each sporocarp represented a single genet in 2001 and 2002, and no identical genets were found between the two years. The results of genetic similarity comparison, using unweighted pair group method with arithmetic means, and analysis of molecular variance, indicated that although genetic variances were mainly within individuals of the same year the genetic variance between years was statistically significant (P<0.001). Relationships between genetic similarity and spatial distance of pairwise sporocarps were also found to be different in the two years. The differences in genetic structure and genetic similarity between individuals of the two years implied that the sporocarps were not likely to be derived from continuous generations, i.e., the sporocarps collected in 2002 were not developed from sexual spores dispersed by sporocarps of 2001. We suggest that the life-cycle traits of ectomycorrhizal (ECM) fungi should be considered in genetic studies on ECM fungal populations.

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Acknowledgements

This work was supported by the State Key Basic Research and Development Plan of China Grant (2002CB111505 and G2000046802), National Natural Science Foundation of China Grant (30070018), and Chinese Academy of Sciences Grant (KSCX2-SW-101C). We are grateful to the Southwest Subalpine Botanical Garden, Chinese Academy of Sciences for providing climate data for the study site and to Dr. Xianming Gao, Dr. Canran Liu, and Dr. Xiaojun Du for providing information on the species composition of the study site. We appreciate comments and suggestions by Professor John Cairney, Professor Andrew Smith and two anonymous reviewers on an earlier version of the manuscript.

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Authors and Affiliations

  1. Laboratory of Quantitative Vegetation Ecology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing , China

    Yu Liang & Ke-ping Ma

  2. Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing , China

    Liang-dong Guo

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  1. Yu Liang
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  2. Liang-dong Guo
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  3. Ke-ping Ma
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Correspondence to Ke-ping Ma.

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Liang, Y., Guo, Ld. & Ma, Kp. Population genetic structure of an ectomycorrhizal fungus Amanita manginiana in a subtropical forest over two years. Mycorrhiza 15, 137–142 (2005). https://doi.org/10.1007/s00572-004-0311-8

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  • DOI: https://doi.org/10.1007/s00572-004-0311-8

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