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Modeling simultaneous glucose and xylose uptake in Saccharomyces cerevisiae from kinetics and gene expression of sugar transporters

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Abstract

A kinetic model for glucose and xylose co-substrate uptake in Saccharomyces cerevisiae is presented. The model couples the enzyme kinetics with the glucose-dependent genetic expression of the individual transport proteins. This novel approach implies several options for optimizing the co-substrate utilization. Interestingly, the simulations predict a maximum xylose uptake rate at a glucose concentration >0 g/L, which suggests that the genetic expressions of the considered transport proteins are of importance when optimizing the xylose uptake. This was also evident in fed-batch simulations, where a distinct optimal glucose addition rate >0 g/L·h was found. Strategies for improving the co-substrate utilization by genetic engineering of the transport systems are furthermore suggested based on simulations.

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Authors and Affiliations

  1. Department of Chemical Engineering, Lund University, Box 124, 221 00, Lund, Sweden

    Magnus Bertilsson, Jonas Andersson & Gunnar Lidén

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  1. Magnus Bertilsson
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  2. Jonas Andersson
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  3. Gunnar Lidén
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Correspondence to Magnus Bertilsson.

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Magnus Bertilsson and Jonas Andersson contributed equally to the work.

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Bertilsson, M., Andersson, J. & Lidén, G. Modeling simultaneous glucose and xylose uptake in Saccharomyces cerevisiae from kinetics and gene expression of sugar transporters. Bioprocess Biosyst Eng 31, 369–377 (2008). https://doi.org/10.1007/s00449-007-0169-1

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  • DOI: https://doi.org/10.1007/s00449-007-0169-1

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