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Evaluation of repellent properties of botanical extracts against Culex tritaeniorhynchus Giles (Diptera: Culicidae)

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Abstract

In recent times, there were considerable efforts made to promote the use of environmentally friendly and biodegradable natural insecticides and repellents, particularly from botanical sources. The present study explored the effects of crude leaf ethyl acetate, acetone, and methanol extracts of Aegle marmelos (L.) Correa ex Roxb, Andrographis lineata Wallich ex Nees., Andrographis paniculata (Burm.f.) Wallich ex Nees., Cocculus hirsutus (L.) Diels, Eclipta prostrata L., and Tagetes erecta L. on repellent activity against Culex tritaeniorhynchus Giles. The maximum repellent activity was observed at 500 ppm in methanol extracts of A. marmelos, ethyl acetate extracts of A. lineata, C. hirsutus, and E. prostrata and the mean complete protection time ranged from 120 to 150 min with the different extracts tested. The ethyl acetate extract of A. lineata showed 100% repellency in 120 min; acetone extracts of A. marmelos and C. hirsutus and methanol extract of T. erecta showed complete protection in 90 min at 250 ppm, respectively. These results suggest that the leaf extracts of A. marmelos, A. lineata, and C. hirsutus have the potential to be used as an ideal eco-friendly approach for the control of the C. tritaeniorhynchus. Therefore, this study provides first report on the repellent activity against Japanese encephalitis, C. tritaeniorhynchus of plant extracts from Southern India.

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References

  • Aguilar HH, de Gives PM, Sánchez DO, Arellano ME, Hernández EL, Aroche UL, Valladares-Cisneros G (2008) In vitro nematocidal activity of plant extracts of Mexican flora against Haemonchus contortus fourth larval stage. Ann N Y Acad Sci 1149:158–160

    Article  PubMed  Google Scholar 

  • Amer A, Mehlhorn H (2006a) Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitol Res 99:466–472

    Article  PubMed  Google Scholar 

  • Amer A, Mehlhorn H (2006b) Repellency effect of forty-one essential oils against Aedes, Anopheles, and Culex mosquitoes. Parasitol Res 99(4):478–490

    Article  PubMed  Google Scholar 

  • Bagavan A, Kamaraj C, Rahuman AA, Elango G, Zahir AA, Pandiyan G (2009) Evaluation of larvicidal and nymphicidal potential of plant extracts against Anopheles subpictus Grassi, Culex tritaeniorhynchus Giles and Aphis gossypii Glover. Parasitol Res 104:1109–1117

    Article  CAS  PubMed  Google Scholar 

  • Bright AA, Babu A, Ignacimuth S, Dorn S (2001) Efficacy of crude extracts of Andrographis paniculata nees. on Callosobruchus chinensis L. during post harvest storage of cowpea. Indian J Exp Biol 39:715–718

    CAS  PubMed  Google Scholar 

  • Broussalis AM, Ferraro GE, Martino VS, Pinzón R, Coussio JD, Alvarez JC (1999) Argentine plants as potential source of insecticidal compounds. J Ethnopharmacol 67:219–223

    Article  CAS  PubMed  Google Scholar 

  • Burkill IH (1966) A dictionary of the economic products of the Malay Peninsula, vol I & II. Ministry of Agriculture and Co-operatives, Kuala Lumpur, p 2444

    Google Scholar 

  • Casida JE, Quistad GB (2000) Insecticide targets: learning to keep up with resistance and changing concepts of safety. Agric Chem Biotechnol 43:185–191

    CAS  Google Scholar 

  • Curtis CF, Lines JD, Baolin L, Renz A (1991) Natural and synthetic repellents. In: Curtis CF (ed) Control of disease vectors in the community. Wolf, London, pp 75–92

    Google Scholar 

  • Dutta A, Sukul NC (1982) Filaricidal properties of a wild herb, Andrographis paniculata. J Helminthol 56:81–84

    Article  CAS  PubMed  Google Scholar 

  • Elango G, Rahuman AA, Bagavan A, Kamaraj C, Zahir AA, Venkatesan C (2009a) Laboratory study on larvicidal activity of indigenous plant extracts against Anopheles subpictus and Culex tritaeniorhynchus. Parasitol Res 104:1381–1388

    Article  CAS  PubMed  Google Scholar 

  • Elango G, Bagavan A, Kamaraj C, Zahir AA, Rahuman AA (2009b) Oviposition-deterrent, ovicidal, and repellent activities of indigenous plant extracts against Anopheles subpictus Grassi (Diptera: Culicidae). Parasitol Res 105:1567–1576

    Article  CAS  PubMed  Google Scholar 

  • Elango G, Rahuman AA, Bagavan A, Kamaraj C, Zahir AA, Rajakumar G, Marimuthu S, Santhoshkumar T (2010) Efficacy of botanical extracts against Japanese encephalitis vector, Culex tritaeniorhynchus. Parasitol Res 106(2):481–492

    Article  PubMed  Google Scholar 

  • Fradin MS, Day JF (2002) Comparative efficacy of insect repellents against mosquito bite. New Engl J Med 347:13–18

    Article  CAS  PubMed  Google Scholar 

  • Ganapaty S, Dash GK, Subburaju T, Suresh P (2002) Diuretic, laxative and toxicity studies of Cocculus hirsutus aerial parts. Fitoterapia 73:28–31

    Article  CAS  PubMed  Google Scholar 

  • Govere J, Braack LEO, Durrheim DN, Hunt RH, Coetzee M (2001) Repellent effects on Anopheles arabiensis biting humans in Kruger Park, South Africa. Med Vet Entomol 15(3):287–292

    Article  CAS  PubMed  Google Scholar 

  • Govindarajan M (2009) Bioefficacy of Cassia fistula Linn. (Leguminosae) leaf extract against chikungunya vector, Aedes aegypti (Diptera: Culicidae). Eur Rev Med Pharmacol Sci 13:99–103

    CAS  PubMed  Google Scholar 

  • Jaenson TG, Pålsson K, Borg-Karlson AK (2006) Evaluation of extracts and oils of mosquito (Diptera: Culicidae) repellent plants from Sweden and Guinea-Bissau. J Med Entomol 43:113–119

    Article  CAS  PubMed  Google Scholar 

  • Kamaraj C, Rahuman AA, Bagavan A (2008) Antifeedant and larvicidal effects of plant extracts against Spodoptera litura (F.), Aedes aegypti L. and Culex quinquefasciatus Say. Parasitol Res 103(2):325–331

    Article  CAS  PubMed  Google Scholar 

  • Kamaraj C, Bagavan A, Rahuman AA, Zahir AA, Elango G, Pandiyan G (2009) Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitol Res 104(5):1163–1171

    Article  CAS  PubMed  Google Scholar 

  • Karunamoorthi K, Mulelam A, Wassie F (2008a) Laboratory evaluation of traditional insect/mosquito repellent plants against Anopheles arabiensis, the predominant malaria vector in Ethiopia. Parasitol Res 103(3):529–534

    Article  PubMed  Google Scholar 

  • Karunamoorthi K, Ramanujam S, Rathinasamy R (2008b) Evaluation of leaf extracts of Vitex negundo L. (Family: Verbenaceae) against larvae of Culex tritaeniorhynchus and repellent activity on adult vector mosquitoes. Parasitol Res 103:545–550

    Article  PubMed  Google Scholar 

  • Khanna VG, Kannabiran K, Getti G (2009) Leishmanicidal activity of saponins isolated from the leaves of Eclipta prostrata and Gymnema sylvestre. Indian J Pharmacol 41(1):32–35

    Article  CAS  PubMed  Google Scholar 

  • Lee SE, Kim JE, Lee HS (2001) Insecticide resistance in increasing interest. Agric Chem Biotechnol 44:105–112

    CAS  Google Scholar 

  • Liu N, Xu Q, Zhu F, Zhang L (2006) Pyrethroid resistance in mosquitoes. Insect Sci 13:159–166

    Article  Google Scholar 

  • Mehlhorn H, Schmahl G, Schmidt J (2005) Extract of the seeds of the plant Vitex agnus castus proven to be highly efficacious as a repellent against ticks, fleas, mosquitoes and biting flies. Parasitolo Res 95:363–365

    Article  Google Scholar 

  • Mullai K, Jebanesan A, Pushpanathan T (2008) Mosquitocidal and repellent activity of the leaf extract of Citrullus vulgaris (cucurbitaceae) against the malarial vector, Anopheles stephensi liston (diptera culicidae). Eur Rev Med Pharmacol Sci 12(1):1–7

    CAS  PubMed  Google Scholar 

  • Natarajana N, Corkb A, Boomathia N, Pandia R, Velavana S, Dhakshnamoorthy G (2006) Cold aqueous extracts of African marigold, Tagetes erecta for control tomato root knot nematode, Meloidogyne incognita. Crop Protection 25:1210–1213

    Article  Google Scholar 

  • Pitasawat B, Choochote W, Tuetun B, Tippawangkosol P, Kanjanapothi D, Jitpakdi A, Riyong D (2003) Repellency of aromatic turmeric Curcuma aromatica under laboratory and field conditions. J Vector Ecol 28:234–240

    PubMed  Google Scholar 

  • Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K (2008) Isolation and identification of mosquito larvicidal compound from Abutilon indicum (Linn.) Sweet. Parasitol Res 102(5):981–988

    Article  PubMed  Google Scholar 

  • Rahuman AA, Bagavan A, Kamaraj C, Saravanan E, Zahir AA, Elango G (2009) Efficacy of larvicidal botanical extracts against Culex quinquefasciatus Say (Diptera: Culicidae). Parasitol Res 104:1365–1372

    Article  CAS  PubMed  Google Scholar 

  • Ramya S, Jayakumararaj R (2009) Antifeedant Activity of Selected Ethno-botanicals used by Tribals of Vattal Hills on Helicoverpa armigera (Hübner). J Pharm Res 2(8):1414–1418

    Google Scholar 

  • Ravi V, Vanajakshi S, Gowda A, Chandramuki A (1989) A laboratory diagnosis of Japanese encephalitis using monoclonal antibodies and correlation of findings with the outcome. J Med Virol 29:221–223

    Article  CAS  PubMed  Google Scholar 

  • Reuben R, Gajanana A (1997) Japanese encephalitis in India. Indian J Pediatr 64:243–251

    Article  CAS  PubMed  Google Scholar 

  • Samarasekera JK, Khambay BP, Hemalal KP (2004) A new insecticidal protolimonoid from Aegle marmelos. Nat Prod Res 18(2):117–122

    Article  CAS  PubMed  Google Scholar 

  • Sarkari NBS, Barthwal SP, Gupta AK, Bagga MMS, Mishra SN,Mishra VK (1984) A clinical appraisal of two epidemics ofJapanese encephalitis in eastern Utter Pradesh. In: Proceedings of National conference on Japanese encephalitis, pp 34–40

  • Sawangjaroen N, Subhadhirasakul S, Phongpaichit S, Siripanth C, Jamjaroen K, Sawangjaroen K (2005) The in vitro anti-giardial activity of extracts from plants that are used for self-medication by AIDS patients in southern Thailand. Parasitol Res 95(1):17–21

    Article  CAS  PubMed  Google Scholar 

  • Singh RN, Saratchandra B (2005) The development of botanical products with special reference to seri-ecosystem. Caspian J Env Sci 3:1–8

    CAS  Google Scholar 

  • Sokal RR, Rohlf JF (1981) Biometry principles and practice of statistics in biological research, 2nd edn. WH Freeman and Company, New York

    Google Scholar 

  • Sugati SS, Sudjaswadi W, Rini S, Wien W (1999) Andrographis paniculata (Burm.f.) Wallich ex Nees. In: de Padua LS, Bunyapraphatsara N, Lemmens RHMJ (eds) PROSEA—plant resources of Southeast Asia No. 12(1). Medicinal and poisonous plants 1. Backhuys, Leiden, pp 119–123

    Google Scholar 

  • Suman DS, Shrivastava AR, Parashar BD, Pant SC, Agrawal OP, Prakash S (2008) Scanning electron microscopic studies on egg surface morphology and morphometrics of Culex tritaeniorhynchus and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 104(1):173–176

    Article  CAS  PubMed  Google Scholar 

  • Tawatsin A, Asavadachanukorn P, Thavara U, Wongsinkongman P, Bansidhi J, Boonruad T, Chavalittumrong P, Soonthornchareon N, Komalamisra N, Mulla MS (2006) Repellency of essential oils extracted from plants in Thailand against four mosquito vectors (Diptera: Culicidae) and oviposition deterrent effects against Aedes aegypti (Diptera: Culicidae). Southeast Asian J Trop Med Public Health 37:915–931

    CAS  PubMed  Google Scholar 

  • Tawatsin A, Wratten SD, Scott RR, Thavara U, Techandamrongsin Y (2001) Repellency of volatile oils from plants against three mosquito vectors. J Vector Ecol 26:76–82

    CAS  PubMed  Google Scholar 

  • Tyagi BK, Ramnath T, Shahi AK (1994) Evaluation of repellency effect of Tagetus minuta (Family: Compositae) against the vector mosquitoes Anopheles stephensi Liston. Culex quinquefasciatus Say and Aedes aegypti (L.). Int Pest Contr 39:48

    Google Scholar 

  • Venkatachalam MR, Jebanesan A (2001) Repellent activity of Ferronia elephantum Corr. (Rutaceae) leaf extract against Aedes aegypti (L.). Bioresour Technol 76:287–288

    Article  CAS  PubMed  Google Scholar 

  • Yang YC, Lee SG, Lee HK, Kim MK, Lee SH, Lee HS (2002) A piperidine amide extracted from Piper longum L. fruit shows activity against Aedes aegypti mosquito larvae. J Agric Food Chem 50:3765–3767

    Article  CAS  PubMed  Google Scholar 

  • Zaridah MZ, Idid SZ, Omar AW, Khozirah S (2001) In vitro antifilarial effects of three plant species against adult worms of subperiodic Brugia malayi. J Ethnopharmacol 78:79–84

    Article  CAS  PubMed  Google Scholar 

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Acknowledgment

The authors are grateful to C. Abdul Hakeem College Management, Dr. S. Mohammed Yousuff, Principal, Dr. K. Abdul Subhan, Reader, and HOD of Zoology Department, for their help and suggestion.

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Authors and Affiliations

  1. Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509, Vellore District, Tamil Nadu, India

    Gandhi Elango, Abdul Abdul Rahuman, Abdul Abduz Zahir, Chinnaperumal Kamaraj, Asokan Bagavan, Govindasamy Rajakumar, Chidambaram Jayaseelan, Thirunavukkarasu Santhoshkumar & Sampath Marimuthu

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  1. Gandhi Elango
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  2. Abdul Abdul Rahuman
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  3. Abdul Abduz Zahir
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  4. Chinnaperumal Kamaraj
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  5. Asokan Bagavan
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  6. Govindasamy Rajakumar
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  7. Chidambaram Jayaseelan
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  8. Thirunavukkarasu Santhoshkumar
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  9. Sampath Marimuthu
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Correspondence to Abdul Abdul Rahuman.

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Elango, G., Rahuman, A.A., Zahir, A.A. et al. Evaluation of repellent properties of botanical extracts against Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitol Res 107, 577–584 (2010). https://doi.org/10.1007/s00436-010-1897-8

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  • DOI: https://doi.org/10.1007/s00436-010-1897-8

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