Abstract
Although the importance of glycoprotein Duffy in the human red cells invasion process by Plasmodium vivax merozoites has been demonstrated, little is known about the associations of FY polymorphisms with malaria vivax parasitic density. In this study, we investigated the associations of the SNPs 125 G>A, 265 C>T, and 298 G>A on FY gene and the SNP –33T>C on GATA box with the vivax malaria parasitic density in inhabitants of Amazon State, Brazil. Verifications of P. vivax, as well as the definition of parasitism, were determined by standard screening tests in 497 patients. FY phenotyping was performed in all samples by hemagglutination using gel cards. Molecular analysis for FY/GATA polymorphisms were performed by polymerase chain reaction–restriction fragment length polymorphism. Our data showed that in this population, FY*A/FY*B-33 and FY*B/FY*B-33 genotypes may be a selective advantage, reducing the frequency of P. vivax infection in the studied area. FY*A/FY*B and FY*A/FY*A genotypes showed to be associated with the rise of the frequency of P. vivax infection, and FY*B/FY*X and FY*A/FY*X showed to be associated with the low levels of parasitism. These results suggest that natural adaptations, in malaria-endemic regions, could be leading to the arising of partial defense mechanisms against P. vivax, which is different from the previously described in African descents, as well as adaptations that could be increasing the susceptibility of human to this kind of malaria.
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References
Albuquerque SRL (2003) Frequency of ABO, Rh, Kell, Kidd, Duffy and MNS blood group systems, in inhabitants from an endemic area of malaria in Amazonas State. Manaus, Dissertation, São Paulo Federal University
Alecrim MGC (2000) Clinical study, parasitic resistency and polymorphism in malaria caused by Plasmodium vivax in Manaus. Thesis, Amazonas Federal University
Barnwell JW, Wartheimer SP (1989) Plasmodium vivax: merozoite antigens, the Duffy blood group, and erythrocyte invasion. Prog Clin Biol Res 313:1–11
Brasil (2008) Health ministry. general office of health vigilance. Information system of epidemiologic vigilance—SIVEP. https://sis.funasa.gov.br/sivep_malaria. Accessed 22 Feb 2008
Bruce MC, Day KP (2003) Cross species regulation of Plasmodium parasitemia in semi-immune children from Papua New Guinea. Trends Parasitol 19:271–277
Castilho L, Rios M, Pellegrino J, Saad ST, Costa FF, Reid ME (2004) A novel FY Allele in Brazilians. Vox Sang 87:190–195
Cavasini CE, De Mattos LC, Couto AA, Couto VS, Gollino Y, Moreti LJ, Bonini-Domingos CR, Rossit AR, Castilho L, Machado RL (2007) Duffy blood group gene polymorphisms among malaria vivax patients in four areas of the Brazilian Amazon Region. Malar J 6:167
Cole-Tobian JL, Michon P, Dabod E, Mueller I, King CL (2007) Dynamics of asymptomatic Plasmodium vivax infection and Duffy binding protein polymorphisms in relation to parasitemia levels in Papua New Guinea children. Am J Trop Med Hyg 77:955–962
Dvorak JA, Miller LH, Whitehouse WC, Shiroishi T (1975) Invasion of erythrocytes by malaria merozoites. Science 187:748–750
Estalote AC, Proto Siqueira R, Silva WA, Zago MA, Palatinik M (2005) The mutation G298A Ala100Thr on the coding sequence of the Duffy antigen/chemokine receptor gene in non-caucasian Brazilians. Genet Mol Res 4:166–173
Fleiss JL (1981) Statistical methods for rates and proportions. Wiley, New York
Gautret P, Legros F, Koulmann P, Rodier MH, Jacquemin JL (2001) Imported Plasmodium vivax malaria in France: geographical origin and report of an atypical case acquired in Central or Western Africa. Acta Trop 78:177–181
Kasehagen LJ, Mueller I, Kiniboro B, Bockarie MJ, Reeder JC, Kazura JW, Kastens W, Mcnamara DT, King CH, Whalen CC, Zimmerman PA (2007) Reduced Plasmodium vivax erythrocyte in PNG Duffy-negative heterozygotes. Plos One 2(3):e336
Langhi DM, Bordin JO (2006) Duffy blood group and malaria. Hematology 11:389–398
Mallinson G, Soo KS, Schall TJ et al (1995) Mutations in the erythrocyte chemokine receptor (Duffy) gene: the molecular basis of the Fya/Fyb antigens and identification of a deletion in the Duffy gene of an apparently healthy individual with the Fy(a-b-) phenotype. Br J Haematol 90:823–829
Mcqueen PG, Mckenzie FE (2006) Competition for red blood cells can enhance Plasmodium vivax parasitemia in mixed-species malaria infections. Am J Trop Med Hyg 1:112–125
Michon P, Wooley I, Wood EM, Kastens W, Zimmerman PA, Adams JS (2001) Duffy-null promoter heterozygozity reduces DARC expression and abrogates adhesion of the P. vivax ligand required for blood-stage infection. FEBS Lett 459:111–114
Miller LH, Mcauliffe FM, Mason SJ (1977) Erythrocyte receptor for malaria merozoites. Am J Trop Med Hyg 26:204–208
Miller LH (1994) Impact of malaria on genetic polymophism and genetic diseases in Africans and African Americans. Proc Natl Acad Sci USA 91:2415–2419
OPAS (2004) Organización Pan-americana de la Saúde. Normas de protocolos genéricos para la eficácia de la cloroquina para el tratamento de la malaria causada pelo P. vivax. Organización Pan-americana da la Saude, Washington
Parasol N, Reid M, Rios M, Castilho L, Harari I, Kosower NS A (1998) Novel mutation in the coding sequence of the FY*B allele of the Duffy chemokine receptor gene is associated with an altered erythrocyte phenotype. Blood 92:2237–2243
Pasvol G (2007) Eroding the resistance of Duffy negativity to invasion by Plasmodium vivax? Trans R Soc Trop Med Hyg 101:953–954
Ryan JR, Stoute JA, Amon J, Dunton RF, Mtalib R, Koros J, Owour B, Luckhart S, Wirtz RA, Barnwell JW, Rosenberg R (2006) Evidence for transmission of Plasmodium vivax among a duffy antigen negative population in western Kenya. Am J Trop Med Hyg 75:575–581
Tournamille C, Colin Y, Cartron JP, Kim CLV (1995) Disruption of a GATA motif in the Duffy gene promoter abolishes erythroid gene expression in Duffy-negative individual. Nat Genet 10:224–228
WHO World Health Organization (2008) World malaria report
Woolley IJ, Hotmire KA, Sramkoski RM, Zimmerman PA, Kazura JW (2000) Differential expression of the Duffy antigen receptor for chemokines according to RBC age and FY genotype. Transfusion 40:949–953
Yazdanbakhsh K, Rios M, Storry JR, Kosower N, Parasol N, Chauduri A, Reid ME (2000) Molecular mechanisms that lead to reduce expression of duffy antigens. Transfusion 40:310–320
Acknowledgments
Spartaco Astofi Filho, Dr. Ph.D. (UFAM), Leny da Mota Passos, Msc (HEMOAM), Franklin Simões, MSc (FMTAM), Marli M. Marques (FMTAM), Edalton Cesar Bezerra Sanguino (FAPEAM), Luciana Correa Tezza (FAPEAM), Laise Kelman Costa Magalhães (FAPEAM), Fernanda Chacon (FAPEAM), Karina (UNICAMP), Débora (UNICAMP), Felician (HEMOAM), Edson da Fonseca de Lira (HEMOAM).
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1. Financiadora de Estudos e Projetos—FINEP do Ministério da Ciência e Tecnologia—MCT
2. Fundação de Amparo à Pesquisa do Estado do Amazonas—FAPEAM
3. Hemocentro do Amazonas—HEMOAM
4. Universidade do Estado do Amazonas—UFAM
5. DiaMed Latino América
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Albuquerque, S.R.L., de Oliveira Cavalcante, F., Sanguino, E.C. et al. FY polymorphisms and vivax malaria in inhabitants of Amazonas State, Brazil. Parasitol Res 106, 1049–1053 (2010). https://doi.org/10.1007/s00436-010-1745-x
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DOI: https://doi.org/10.1007/s00436-010-1745-x