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Evaluation of baculovirus-derived recombinant 53-kDa protein of Trichinella spiralis for detection of Trichinella-specific antibodies in domestic pigs by ELISA

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Abstract

The complete gene encoding the 53-kDa protein derived from Trichinella spiralis was cloned and expressed using a baculovirus-based system. Characterization of a purified fusion protein consisting of the 53-kDa protein and the glutathione S-transferase protein showed unspecific reactivity with swine pre-immune serum in both enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Subsequently, a purified C-terminal 6×His-tagged 53-kDa protein was used in an ELISA. The evaluation of the test using a negative serum panel showed a high specificity for the ELISA. Serum panels of pigs infected with T. spiralis of two independent experiments showed that pigs of one experiment were tested positive by the ELISA, whereas all sera of the second experiment were negative, indicating a low sensitivity of the ELISA. Furthermore, experimental evidence was found by using mass spectroscopy and Western blot analysis that the 53-kDa protein was not part of the excretory/secretory antigen of T. spiralis as shown in this study.

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Acknowledgements

The project was funded by RIEMSER Arzneimittel AG, the European Fund for Regional Development (EFRE) and the German federal state Mecklenburg-Western Pomerania.

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Authors and Affiliations

  1. Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493, Greifswald-Insel Riems, Germany

    Doreen Jung, Jens Peter Teifke, Axel Karger, Kathrin Michael & Egbert Mundt

  2. Institute for Medical Biochemistry and Molecular Biology, University of Greifswald, 17491, Greifswald, Germany

    Simone Venz

  3. Riemser Arzneimittel AG, 17493, Greifswald-Insel Riems, Germany

    Wolfgang Wittmann & Katharina Kindermann

  4. Federal Institute for Risk Assessment, 12277, Berlin, Germany

    Karsten Nöckler

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  1. Doreen Jung
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  2. Jens Peter Teifke
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  3. Axel Karger
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  4. Kathrin Michael
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  5. Simone Venz
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  8. Karsten Nöckler
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  9. Egbert Mundt
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Correspondence to Egbert Mundt.

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Jung, D., Teifke, J.P., Karger, A. et al. Evaluation of baculovirus-derived recombinant 53-kDa protein of Trichinella spiralis for detection of Trichinella-specific antibodies in domestic pigs by ELISA. Parasitol Res 100, 429–437 (2007). https://doi.org/10.1007/s00436-006-0296-7

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  • DOI: https://doi.org/10.1007/s00436-006-0296-7

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