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Molecular epidemiology of HIV in a cohort of men having sex with men from Istanbul

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Abstract

In Turkey, the first HIV/AIDS case was reported in 1985. Since then the number of persons with HIV infection has increased, HIV is getting a public health problem. The aim of this study was to determine HIV-1 subtype diversity, drug resistance and gag cleavage site mutations among 20 HIV-infected men having sex with men from Istanbul, Turkey. The most prevalent subtype was found to be subtype B (50 %), but also the non-B subtypes A1, C and CRF02_AG, CRF03_AB and CRF06_cpx were found. Resistance-associated mutations were found in 6 patients (30 %) with 2/6 patients being therapy-experienced and 4/6 therapy-naïve at the time-point of analysis. In these patients, the nucleoside reverse transcriptase inhibitor (NRTI)-associated resistance mutations M41L, T215C, V75I, T69N, the non-NRTI associated mutations V106I, E138A, K103N and the protease inhibitor associated mutations Q58E and V82I were detected. Two virus strains also presented Gag cleavage site mutations. With increasing numbers of HIV-infected Turkish patients that require anti-retroviral treatment, HIV-1 drug-resistance testing is strongly recommended in order to choose the most active drug combination for therapy to achieve better clinical outcomes.

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Acknowledgments

This study was supported by the Istanbul University Scientific Research Project Nr. 10696 and by the Bundesministerium für Bildung und Forschung (01DL12017). We thank the Positive Living Association Istanbul for their contribution in sample collection.

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Authors and Affiliations

  1. Department of Microbiology and Clinical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey

    Dilek Alpsar, Ali Agacfidan & Emel Bozkaya

  2. Institute of Virology, University of Cologne, Fürst-Pückler-Straße 56, 50935, Köln, Germany

    Nadine Lübke, Jens Verheyen, Rolf Kaiser & Baki Akgül

  3. Department of Infectious Diseases, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey

    Haluk Eraksoy & Atahan Çağatay

Authors
  1. Dilek Alpsar
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  2. Ali Agacfidan
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  3. Nadine Lübke
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  4. Jens Verheyen
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  7. Emel Bozkaya
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  9. Baki Akgül
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Correspondence to Nadine Lübke.

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Alpsar, D., Agacfidan, A., Lübke, N. et al. Molecular epidemiology of HIV in a cohort of men having sex with men from Istanbul. Med Microbiol Immunol 202, 251–255 (2013). https://doi.org/10.1007/s00430-012-0285-7

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  • DOI: https://doi.org/10.1007/s00430-012-0285-7

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