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Gene expression profiling of human hepatoblastoma using archived formalin-fixed and paraffin-embedded tissues

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Abstract

We elucidated the genetic profile of hepatoblastomas (HBLs) to identify diagnostic and prognostic markers. RNA was extracted from 32 formalin-fixed, paraffin-embedded HBLs and corresponding nonneoplastic liver (NNL) tissues, and cDNA-mediated annealing, selection, extension, and ligation (DASL) chip assays were performed. Immunohistochemistry was performed to confirm the expression of Yin Yang 1 (YY1) protein in HBL. Twenty-four genes that were associated with signal transduction, cell–cell adhesion, cell cycle regulation, and apoptosis were differentially expressed in HBL and NNL tissues. Two apoptosis-associated genes, MYCN and BIRC5, were highly upregulated in HBL. Eight genes, including YY1 and IGF1, were upregulated in HBL cases that had a poor prognosis. Thirty-eight genes, including YY1, were differentially expressed according to histologic differentiation of HBL, and the immunohistochemical expression of YY1 was correlated with poor HBL differentiation. Thus, using DASL chip assays, we report the gene expression profiles of HBL, which suggest new candidate prognostic and diagnostic genetic markers and putative therapeutic targets for HBL.

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Acknowledgments

This study was supported by grant 0420090660 from the Clinical Institute of Seoul National University Hospital, Seoul, Republic of Korea.

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The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Pathology, Seoul National University College of Medicine, #28 Yongon-dong, Jongro-gu, Seoul, 110-799, South Korea

    Eun Shin, Kyoung-Bun Lee, Soo-Young Park, Soo-Hee Kim, Han-Suk Ryu & Ja-June Jang

  2. Department of Pathology, Yonsei University College of Medicine, Seoul, South Korea

    Young-Nyun Park

  3. Department of Pathology, Seoul Asan Medical Center, Seoul, South Korea

    Eunsil Yu

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  1. Eun Shin
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Correspondence to Ja-June Jang.

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Shin, E., Lee, KB., Park, SY. et al. Gene expression profiling of human hepatoblastoma using archived formalin-fixed and paraffin-embedded tissues. Virchows Arch 458, 453–465 (2011). https://doi.org/10.1007/s00428-011-1043-8

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  • DOI: https://doi.org/10.1007/s00428-011-1043-8

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