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Genome-wide identification of BURP domain-containing genes in rice reveals a gene family with diverse structures and responses to abiotic stresses

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Abstract

Increasing evidence suggests that a gene family encoding proteins containing BURP domains have diverse functions in plants, but systematic characterization of this gene family have not been reported. In this study, 17 BURP family genes (OsBURP01–17) were identified and analyzed in rice (Oryza sativa L.). These genes have diverse exon–intron structures and distinct organization of putative motifs. Based on the phylogenetic analysis of BURP protein sequences from rice and other plant species, the BURP family was classified into seven subfamilies, including two subfamilies (BURP V and BURP VI) with members from rice only and one subfamily (BURP VII) with members from monocotyledons only. Two BURP gene clusters, belonging to BURP V and BURP VI, were located in the duplicated region on chromosome 5 and 6 of rice, respectively. Transcript level analysis of BURP genes of rice in various tissues and organs revealed different tempo-spatial expression patterns, suggesting that these genes may function at different stages of plant growth and development. Interestingly, all the genes of the BURP VII subfamily were predominantly expressed in flower organs. We also investigated the expression patterns of BURP genes of rice under different stress conditions. The results suggested that, except for two genes (OsBURP01 and OsBURP13), all other members were induced by at least one of the stresses including drought, salt, cold, and abscisic acid treatment. Two genes (OsBURP05 and OsBURP16) were responsive to all the stress treatments and most of the OsBURP genes were responsive to salt stress. Promoter sequence analysis revealed an over-abundance of stress-related cis-elements in the stress-responsive genes. The data presented here provide important clues for elucidating the functions of genes of this family.

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Abbreviations

ABA:

Abscisic acid

ABRE:

ABA responsive element

BURP:

BNM2, USP, RD22, and PG1β

DRE:

Dehydration-responsive element

LTRE:

Low temperature-responsive element

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Acknowledgments

This work was supported by the grants from the National Program on the Development of Basic Research, the National Program on High Technology Development, the National Natural Science Foundation, and the Ministry of Education of China.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China

    Xipeng Ding, Xin Hou, Kabin Xie & Lizhong Xiong

  2. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, China

    Lizhong Xiong

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  1. Xipeng Ding
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  2. Xin Hou
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  3. Kabin Xie
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  4. Lizhong Xiong
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Correspondence to Lizhong Xiong.

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X. Ding and X. Hou contributed equally to this work.

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Ding, X., Hou, X., Xie, K. et al. Genome-wide identification of BURP domain-containing genes in rice reveals a gene family with diverse structures and responses to abiotic stresses. Planta 230, 149–163 (2009). https://doi.org/10.1007/s00425-009-0929-z

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