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Investigating the mechanisms of glyphosate resistance in Lolium multiflorum

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Abstract

Evolved resistance to the herbicide glyphosate has been reported in eleven weed species, including Lolium multiflorum. Two glyphosate-resistant L. multiflorum populations were collected, one from Chile (SF) and one from Oregon, USA (OR), and the mechanisms conferring glyphosate resistance were studied. Based on a Petri dish dose–response bioassay, the OR and the SF populations were two and fivefold more resistant to glyphosate when compared to the susceptible (S) population, respectively; however, based on a whole-plant dose–response bioassay, both OR and SF populations were fivefold more resistant to glyphosate than the S population, implying that different resistance mechanisms might be involved. The S population accumulated two and three times more shikimic acid in leaf tissue 96 h after glyphosate application than the resistant OR and SF populations, respectively. There were no differences between the S and the glyphosate-resistant OR and SF populations in 14C-glyphosate leaf uptake; however, the patterns of 14C-glyphosate translocation were significantly different. In the OR population, a greater percentage of 14C-glyphosate absorbed by the plant moved distal to the treated section and accumulated in the tip of the treated leaf. In contrast, in the S and in the SF populations, a greater percentage of 14C-glyphosate moved to non-treated leaves and the stem. cDNA sequence analysis of the EPSP synthase gene indicated that the glyphosate-resistant SF population has a proline 106 to serine amino acid substitution. Here, we report that glyphosate resistance in L. multiflorum is conferred by two different mechanisms, limited translocation (nontarget site-based) and mutation of the EPSP synthase gene (target site-based).

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Abbreviations

EPSP:

5-Enolpyruvylshikimate-3-phosphate

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Acknowledgments

The 14C-glyphosate leaf uptake and translocation experiments were carried out at Syngenta Vero Beach Research Center. We thank Syngenta Crop Protection for their economic and technical support.

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Authors and Affiliations

  1. Department of Crop and Soil Science, Oregon State University, Corvallis, OR, 97331-3002, USA

    Alejandro Perez-Jones, Kee-Woong Park & Carol A. Mallory-Smith

  2. Syngenta Crop Protection, Inc., Vero Beach Research Center, Vero Beach, FL, 32967, USA

    Nick Polge

  3. Department of Horticulture, University of Wisconsin, Madison, WI, 53706-1590, USA

    Jed Colquhoun

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  1. Alejandro Perez-Jones
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  2. Kee-Woong Park
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  3. Nick Polge
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  5. Carol A. Mallory-Smith
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Correspondence to Alejandro Perez-Jones.

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Perez-Jones, A., Park, KW., Polge, N. et al. Investigating the mechanisms of glyphosate resistance in Lolium multiflorum . Planta 226, 395–404 (2007). https://doi.org/10.1007/s00425-007-0490-6

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