Abstract
Elf5 belongs to the ets family of transcription factors and was cloned by homology in the DNA binding domain to the related, epithelial-specific ets factor, Elf3. Elf5 mRNA is expressed highly in normal tissue rich in secretory epithelial cells, including mammary gland, lung, kidney, prostate, salivary gland and stomach. The function of Elf5 and the cell types in which it is expressed remain uncharacterised. The presence of Elf5 mRNA in normal tissues, but absence in cancer tissues, may suggest a role for Elf5 in differentiation and development. We have generated a rabbit antiserum directed against a peptide in the Elf5 DNA-binding domain that is conserved between murine and human sequences. The antiserum specifically detects human and murine Elf5 proteins on western blots and shows specific staining on paraffin-embedded sections obtained from tissues including mammary gland, kidney, salivary gland and stomach. Epithelia from the bladder lining, lung and prostate did not stain for the presence of Elf5, though these organs express Elf5 mRNA. We show for the first time that Elf5 is primarily expressed in epithelial cells and is likely to be an epithelial-specific protein. The antiserum should prove useful in further analysis of the expression and function of Elf5.
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Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Brembeck F, Opitz OG, Libermann TA, Rustgi AK (2000) Dual function of the epithelial specific ets transcription factor, ELF3, in modulating differentiation. Oncogene 19:1941–1949
Chang C-H, Scott GK, Kuo W-L, Xiong X, Suzdaltseva Y, Park JW, Sayre P, Erny K, Collins C, Gray JW, Benz CC (1997) ESX: a structurally unique Ets overexpressed early during human breast tumorigenesis. Oncogene 14:1617–1622
Chen H, Nandi AK, Li X, Bieberich CJ (2002) NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter. Cancer Res 62:338–340
Harlow E, Lane D (1988) Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Kas K, Finger E, Grall F, Gu X, Akbarali Y, Boltax J, Weiss A, Oettgen P, Kapeller R, Libermann TA (2000) ESE-3, a novel member of an epithelium-specific ets transcription factor subfamily, demonstrates different target gene specificity from ESE-1. J Biol Chem 275:2986–2998
Kleinbaum LA, Duggan C, Ferreira E, Coffey GP, Buttice G, Burton FH (1999) Human chromosomal localization, tissue/tumor expression, and regulatory function of the ets family gene EHF. Biochem Biophys Res Commun 264:119–126
Neve R, Chang C-H, Scott GK, Wong A, Friis RR, Hynes Ne, Benz CC (1998) The epithelium-specific Ets transcription factor ESX is associated with mammary gland development and involution. FASEB J 12:1541–1550
Ng AY, Waring P, Ristevski S, Wang C, Wilson T, Pritchard M, Hertzog P, Kola I (2002) Inactivation of the transcription factor Elf3 in mice results in dysmorphogenesis and altered differentiation of intestinal epithelium. Gastroenterology 122:1455–1466
Nozawa M, Yomogida K, Kanno N, Nonomura N, Miki T, Okuyama A, Nishimune Y, Nozaki M (2000) Prostate-specific transcription factor hPSE is translated only in normal prostate epithelial cells. Cancer Res 60:1348–1352
Oettgen P, Carter KC, Augustus M, Barcinski M, Boltax J, Kunsch C Libermann TA (1997) The novel epithelial-specific Ets transcription factor gene ESX maps to human chromosome 1q32.1. Genomics 445:456–457
Oettgen P, Kas K, Dube A, Gu X, Grall F, Thamrongsak U, Akbarali Y, Finger E, Boltax J, Endress G, Munger K, Kunsch C, Libermann TA (1999) Characterisation of ESE-2, a novel ESE-1-related Ets transcription factor that is restricted to glandular epithelium and differentiated keratinocytes. J Biol Chem 274:29439–29452
Oettgen P, Finger E, Sun Z, Akbarali Y, Thamrongsak U, Boltax J, Grall F, Dube A, Weiss A, Brown L, et al (2000) PDEF, a novel prostate epithelium-specific ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression. J Biol Chem 275:1216–1225
Tymms MJ, Ng AY, Thomas RS, Schutte BC, Zhou J, Eyre HJ, Sutherland GR, Seth A, Rosenberg M, Papas T, et al (1997) A novel epithelial-expressed ETS gene, ELF3: human and murine cDNA sequences, murine genomic organization, human mapping to 1q32.2 and expression in tissues and cancer. Oncogene 15:2449–2462
Wang C-Y, Petryniak B, Ho I-C, Thompson CB, Leiden JM (1992) Evolutionarily conserved Ets family members display distinct DNA binding specificities. J Exp Med 175:1391–1399
Wasylyk C, Kerckaert J-P, Wasylyk B (1992) A novel modulator domain of Ets transcription factors. Genes Dev 6:965–974
Wasylyk B, Hahn SL, Giovane A (1993) The Ets family of transcription factors. Eur J Biochem 211:7–18
Wolvetang EJ, Wilson TJ, Sanij E, Busciglio J, Hatzistavrou T, Seth A, Hertzog PJ, Kola I (2003) ETS2 overexpression in transgenic models and in Down syndrome predisposes to apoptosis via the p53 pathway. Hum Mol Genet 12:247–255
Xu D, Wilson TJ, Chan D, De Luca E, Zhou J, Hertzog PJ, Kola I (2002) Ets1 is required for p53 transcriptional activity in UV-induced apoptosis in embryonic stem cells. EMBO J 21:4081–4093
Yamada N, Tamai Y, Miyamoto H, Nozaki M (2000) Cloning and expression of the mouse Pse gene encoding a novel Ets family member. Gene 241:267–274
Zhou J, Ng AY, Tymms MJ, Jermiin LS, Seth AK, Thomas RS, Kola I (1998) A novel transcription factor, ELF5, belongs to the ELF subfamily of ETS genes and maps to human chromosome 11p13–15, a region subject to LOH and rearrangement in human carcinoma cell lines. Oncogene 17:2719–2732
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The authors would like to thank Stuart Rodda at Mimotopes for his helpful advice on choosing the Elf5 peptide.
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Lapinskas, E.J., Palmer, J., Ricardo, S. et al. A major site of expression of the ets transcription factor Elf5 is epithelia of exocrine glands. Histochem Cell Biol 122, 521–526 (2004). https://doi.org/10.1007/s00418-004-0713-x
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DOI: https://doi.org/10.1007/s00418-004-0713-x