Skip to main content
SpringerLink
Log in

Hypothermia Attenuates Vascular Manifestations of Ventilator-Induced Lung Injury in Rats

  • Published:
Lung Aims and scope Submit manuscript

Abstract

Alveolar hemorrhage and pulmonary edema induced by mechanical ventilation are partly dependent on cardiac output. Because cardiac output is low during hypothermia, we hypothesized that hypothermia may protect against these vascular manifestations of ventilator-induced lung injury. Twenty-seven Sprague-Dawley rats were assigned to either normothermia (37 ± 1°C)-injurious ventilation (NT; n = 10), hypothermia (27 ± 1°C)- injurious ventilation (HT; n = 10), or nonventilated control (n = 7). The two ventilated groups were subjected to injurious ventilation of peak airway pressure 30 cm H2O with zero end-expiratory pressure for 20 min. Compared with the NT group, the hemorrhage/congestion score of the lung (11.2 ± 1.5 vs. 4.7 ± 1.6; p < 0.001) and the ratio of wet/dry lung weight (6.1 ± 0.8 vs. 5.0 ± 0.1; p = 0.046) of the HT group were lower. Compared with the NT group, protein concentration (3,471 ± 1,985 µg/ml vs. 1,374 ± 726 µg/ml; p = 0.003) and lactate dehydrogenase level (0.43 ± 0.22 U/ml vs. 0.18 ± 0.1 U/ml; p = 0.046) in bronchoalveolar lavage fluid of the HT group were lower. Whereas pressure-volume curve was shifted to the right in the NT group after injurious ventilation, it was not shifted in the HT group. In conclusion, hypothermia in rats attenuated the degrees of vascular manifestations and alveolar epithelial injuries induced by injurious ventilation, and preserved the mechanical properties of the lung.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5

Similar content being viewed by others

References

  1. MB Amato CS Barbas DM Medeiros et al. (1998) ArticleTitleEffect of a protective-ventilation strategy on mortality in the acute respiratory distress syndrome. N Engl J Med 338 347–354 Occurrence Handle1:STN:280:DyaK1c7ht1ejtw%3D%3D Occurrence Handle9449727

    CAS  PubMed  Google Scholar 

  2. R Behnia A Molteni CM Waters RJ Panos WF Ward HW Schnaper CH TS'Ao (1996) ArticleTitleEarly markers of ventilator-induced lung injury in rats. Ann Clin Lab Sci 26 437–450 Occurrence Handle1:STN:280:ByiD3szntVA%3D Occurrence Handle8879362

    CAS  PubMed  Google Scholar 

  3. S Bernard (1996) ArticleTitleInduced hypothermia in intensive care medicine. Anaesth Intens Care 24 382–388 Occurrence Handle1:STN:280:BymA1MbjvVI%3D

    CAS  Google Scholar 

  4. WD Biggar D Bohn G Kent (1983) ArticleTitleNeutrophil circulation and release from bone marrow during hypothermia. Infect Immun 40 708–712 Occurrence Handle1:STN:280:BiyC1cvntlU%3D Occurrence Handle6840858

    CAS  PubMed  Google Scholar 

  5. AF Broccard JR Hotchkiss N Kuwayama et al. (1998) ArticleTitleConsequences of vascular flow on lung injury induced by mechanical ventilation. Am J Respir Crit Care Med 157 1935–1942 Occurrence Handle1:STN:280:DyaK1c3osFygug%3D%3D Occurrence Handle9620930

    CAS  PubMed  Google Scholar 

  6. A Broccard RS Shapiro LL Schmitz AB Adams A Nahum JJ Marini (2000) ArticleTitleProne positioning attenuates and redistributes ventilator-induced lung injury in dogs. Crit Care Med 28 295–303 Occurrence Handle1:STN:280:DC%2BD3c7ntVChuw%3D%3D Occurrence Handle10708156

    CAS  PubMed  Google Scholar 

  7. Z Bshouty J Ali M Younes (1988) ArticleTitleEffect of tidal volume and PEEP on rate of edema formation in in situ perfused canine lobes. J Appl Physiol 64 1900–1907 Occurrence Handle1:STN:280:BieB2s3ovVE%3D Occurrence Handle3292503

    CAS  PubMed  Google Scholar 

  8. M Colmenero-Ruiz E Fernandez-Mondejar MA Fernandez-Sacristan R Rivera-Fernandez G Vazquez-Mata (1997) ArticleTitlePEEP and low tidal volume ventilation reduce lung water in porcine pulmonary edema. Am J Respir Crit Care Med 155 964–970 Occurrence Handle1:STN:280:ByiB2MflsF0%3D Occurrence Handle9117033

    CAS  PubMed  Google Scholar 

  9. TC Corbridge LD Wood GP Crawford MJ Chudoba J Yanos JI Sznajder (1990) ArticleTitleAdverse effects of large tidal volume and low PEEP in canine acid aspiration. Am Rev Respir Dis 142 311–315 Occurrence Handle1:STN:280:By%2BA2czhtlM%3D Occurrence Handle2200314

    CAS  PubMed  Google Scholar 

  10. RH Demling NC Staub LH Edmunds Jr (1975) ArticleTitleEffect of end-expiratory airway pressure on accumulation of extravascular lung water. J Appl Physiol 38 907–912 Occurrence Handle1:STN:280:CSqC2M7jvVY%3D Occurrence Handle1126901

    CAS  PubMed  Google Scholar 

  11. D Dreyfuss G Basset P Soler G Saumon (1985) ArticleTitleIntermittent positive-pressure hyperventilation with high inflation pressures produces pulmonary microvascular injury in rats. Am Rev Respir Dis 132 880–888 Occurrence Handle1:STN:280:BimD38zotlY%3D Occurrence Handle3901844

    CAS  PubMed  Google Scholar 

  12. D Dreyfuss G Saumon (1993) ArticleTitleRole of tidal volume, FRC, and end-inspiratory volume in the development of pulmonary edema following mechanical ventilation. Am Rev Respir Dis 148 1194–1203 Occurrence Handle1:STN:280:ByuD2MjmvV0%3D Occurrence Handle8239153

    CAS  PubMed  Google Scholar 

  13. D Dreyfuss G Saumon (1998) ArticleTitleVentilator-induced lung injury. Am J Respir Crit Care Med 157 294–323 Occurrence Handle1:STN:280:DyaK1c7gs1SqsA%3D%3D Occurrence Handle9445314

    CAS  PubMed  Google Scholar 

  14. D Dreyfuss P Soler G Basset G Saumon (1988) ArticleTitleHigh inflation pressure pulmonary edema. Respective effects of high airway pressure, high tidal volume, and positive end-expiratory pressure. Am Rev Respir Dis 137 1159–1164 Occurrence Handle1:STN:280:BiaD28%2Fms1w%3D Occurrence Handle3057957

    CAS  PubMed  Google Scholar 

  15. G Eshel G Reisler M Berkovitch S Shapira E Grauer J Barr (2002) ArticleTitleComparison of fast versus slow rewarming following acute moderate hypothermia in rats. Paediatr Anesth 12 235–242 Occurrence Handle10.1046/j.1460-9592.2002.00801.x

    Article  Google Scholar 

  16. Z Fu ML Costello K Tsukimoto et al. (1992) ArticleTitleHigh lung volume increases stress failure in pulmonary capillaries. J Appl Physiol 73 123–133 Occurrence Handle1:STN:280:By2A2s3lsFE%3D Occurrence Handle1506359

    CAS  PubMed  Google Scholar 

  17. L Gattinoni M Bombino P Pelosi et al. (1994) ArticleTitleLung structure and function in different stages of severe adult respiratory distress syndrome. JAMA 271 1772–1779 Occurrence Handle1:STN:280:ByuB2MvhslY%3D Occurrence Handle8196122

    CAS  PubMed  Google Scholar 

  18. GF Hartree (1972) ArticleTitleDetermination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem 48 422–427 Occurrence Handle1:CAS:528:DyaE38XksVGjur0%3D Occurrence Handle4115981

    CAS  PubMed  Google Scholar 

  19. I Hasinoff J Ducas RM Prewitt (1988) ArticleTitleIncreased cardiac output increases lung water in canine permeability pulmonary edema. J Crit Care 3 225–231

    Google Scholar 

  20. JR Hotchkiss Jr L Blanch G Murias et al. (2000) ArticleTitleEffects of decreased respiratory frequency on ventilator-induced lung injury. Am J Respir Crit Care Med 161 463–468 Occurrence Handle1:STN:280:DC%2BD3c7otVamtw%3D%3D Occurrence Handle10673186

    CAS  PubMed  Google Scholar 

  21. JG Laffey D Engelberts BP Kavanagh (2000) ArticleTitleInjurious effects of hypocapnic alkalosis in the isolated lung. Am J Respir Crit Care Med 162 399–405 Occurrence Handle1:STN:280:DC%2BD3cvjtlCjsA%3D%3D Occurrence Handle10934060

    CAS  PubMed  Google Scholar 

  22. J Mead T Takishima D Leith (1970) ArticleTitleStress distribution in lungs: a model of pulmonary elasticity. J Appl Physiol 28 596–608 Occurrence Handle1:STN:280:CS%2BC2sjntlM%3D Occurrence Handle5442255

    CAS  PubMed  Google Scholar 

  23. Y Moshkovitz TE David M Caleb CM Feindel MP de Sa (1998) ArticleTitleCirculatory arrest under moderate systemic hypothermia and cold retrograde cerebral perfusion. Ann Thorac Surg 66 1179–1184 Occurrence Handle10.1016/S0003-4975(98)00805-4 Occurrence Handle1:STN:280:DyaK1M%2FhsFChsA%3D%3D Occurrence Handle9800803

    Article  CAS  PubMed  Google Scholar 

  24. S Nyren M Mure H Jacobsson SA Larsson SG Lindahl (1999) ArticleTitlePulmonary perfusion is more uniform in the prone than in the supine position: scintigraphy in healthy humans. J Appl Physiol 86 1135–1141 Occurrence Handle1:STN:280:DyaK1M3htVaqug%3D%3D Occurrence Handle10194194

    CAS  PubMed  Google Scholar 

  25. JC Parker MI Townsley B Rippe AE Taylor J Thigpen (1984) ArticleTitleIncreased microvascular permeability in dog lungs due to high peak airway pressures. J Appl Physiol 57 1809–1816 Occurrence Handle1:STN:280:BiqD2sblt10%3D Occurrence Handle6511554

    CAS  PubMed  Google Scholar 

  26. L Puybasset P Cluzel N Chao AS Slutsky P Coriat JJ Rouby (1998) ArticleTitleA computed tomography scan assessment of regional lung volume in acute lung injury. The CT Scan ARDS Study Group. Am J Respir Crit Care Med 158 1644–1655 Occurrence Handle1:STN:280:DyaK1M%2FjsVOmtw%3D%3D Occurrence Handle9817720

    CAS  PubMed  Google Scholar 

  27. T Sakuma T Nishimura K Usude M Handa G Okaniwa S Suzuki S Fujimura (1997) ArticleTitleHypothermia inhibits the alveolar epithelial injury caused by hyposmotic albumin solution during preservation of the resected human lung. Surg Today 27 527–533 Occurrence Handle1:STN:280:ByiH2M7mtVU%3D Occurrence Handle9306546

    CAS  PubMed  Google Scholar 

  28. K Shibata N Cregg D Engelberts A Takeuchi L Fedorko BP Kavanagh (1998) ArticleTitleHypercapnic acidosis may attenuate acute lung injury by inhibition of endogenous xanthine oxidase. Am J Respir Crit Care Med 158 1578–1584 Occurrence Handle1:STN:280:DyaK1M%2FjsVOntA%3D%3D Occurrence Handle9817711

    CAS  PubMed  Google Scholar 

  29. SE Sinclair DA Kregenow WJ Lamm IR Starr EY Chi MP Hlastala (2002) ArticleTitleHypercapnic acidosis is protective in an in vivo model of ventilator-induced lung injury. Am J Respir Crit Care Med 166 403–408 Occurrence Handle10.1164/rccm.200112-117OC Occurrence Handle12153979

    Article  PubMed  Google Scholar 

  30. InstitutionalAuthorNameThe Acute Respiratory Distress Syndrome Network (2000) ArticleTitleVentilation with lower tidal volumes as compared with traditional tidal volumes for acute lung injury and the acute respiratory distress syndrome. N Engl J Med 342 1301–1308 Occurrence Handle10793162

    PubMed  Google Scholar 

  31. T Toung P Saharia S Permutt GD Zuidema JL Cameron (1977) ArticleTitleAspiration pneumonia: beneficial and harmful effects of positive end-expiratory pressure. Surgery 82 279–283 Occurrence Handle1:STN:280:CSiB3MnkvVI%3D Occurrence Handle327600

    CAS  PubMed  Google Scholar 

  32. DJ Tschumperlin J Oswari AS Margulies (2000) ArticleTitleDeformation-induced injury of alveolar epithelial cells. Effect of frequency, duration, and amplitude. Am J Respir Crit Care Med 162 357–362 Occurrence Handle1:STN:280:DC%2BD3cvjtleqtw%3D%3D Occurrence Handle10934053

    CAS  PubMed  Google Scholar 

  33. K Tsuno K Miura M Takeya T Kolobow T Morioka (1991) ArticleTitleHistopathologic pulmonary changes from mechanical ventilation at high peak airway pressures. Am Rev Respir Dis 143 1115–1120 Occurrence Handle1:STN:280:By6B387jslI%3D Occurrence Handle2024823

    CAS  PubMed  Google Scholar 

  34. T Tveita K Ytrehus M Skandfer et al. (1996) ArticleTitleChanges in blood flow distribution and capillary function after deep hypothermia in rat. Can J Physiol Pharm 74 376–381 Occurrence Handle10.1139/cjpp-74-4-376 Occurrence Handle1:CAS:528:DyaK28XksV2jurk%3D

    Article  CAS  Google Scholar 

  35. SM Walther KB Domino RW Glenny MP Hlastala (1999) ArticleTitlePositive end-expiratory pressure redistributes perfusion to dependent lung regions in supine but not in prone lambs. Crit Care Med 27 37–45 Occurrence Handle1:STN:280:DyaK1M7jtFGqsg%3D%3D Occurrence Handle9934891

    CAS  PubMed  Google Scholar 

  36. HH Webb DF Tierney (1974) ArticleTitleExperimental pulmonary edema due to intermittent positive pressure ventilation with high inflation pressures. Protection by positive end-expiratory pressure. Am Rev Respir Dis 110 556–565 Occurrence Handle1:STN:280:CSqD2M3psFA%3D Occurrence Handle4611290

    CAS  PubMed  Google Scholar 

  37. C Wenisch E Narzt DI Sessler et al. (1996) ArticleTitleMild intraoperative hypothermia reduces production of reactive oxygen intermediates by polymorphonuclear leukocytes. Anesth Analg 82 810–816 Occurrence Handle1:STN:280:BymB3c7otlc%3D Occurrence Handle8615502

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This study was supported by a fund from the Asan Institute for Life Sciences (#2001-161) and a fund from the Korean Academy of Tuberculosis and Respiratory Diseases (2000).

Author information

Authors and Affiliations

  1. Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea 138-600

    C. -M. Lim, S. -B. Hong, Y. Koh, S. D. Lee, W. S. Kim, D. -S. Kim & W. D. Kim

Authors
  1. C. -M. Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. -B. Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Koh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. D. Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. W. S. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. -S. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. W. D. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. -M. Lim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lim, CM., Hong, SB., Koh, Y. et al. Hypothermia Attenuates Vascular Manifestations of Ventilator-Induced Lung Injury in Rats . Lung 181, 23–34 (2003). https://doi.org/10.1007/s00408-002-0111-x

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00408-002-0111-x

Keywords

Search

Navigation