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Cloning, tissue expression pattern and daily rhythms of Period1, Period2, and Clock transcripts in the flatfish Senegalese sole, Solea senegalensis

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Abstract

An extensive network of endogenous oscillators governs vertebrate circadian rhythmicity. At the molecular level, they are composed of a set of clock genes that participate in transcriptional–translational feedback loops to control their own expression and that of downstream output genes. These clocks are synchronized with the environment, although entrainment by external periodic cues remains little explored in fish. In this work, partial cDNA sequences of clock genes representing both positive (Clock) and negative (Period1, Period2) elements of the molecular feedback loops were obtained from the nocturnal flatfish Senegalese sole, a relevant species for aquaculture and chronobiology. All of the above genes exhibited high identities with their respective teleost clock genes, and Per–Arnt–Sim or basic helix–loop–helix binding domains were recognized in their primary structure. They showed a widespread distribution through the animal body and some of them displayed daily mRNA rhythms in central (retina, optic tectum, diencephalon, and cerebellum) and peripheral (liver) tissues. These rhythms were most robust in retina and liver, exhibiting marked Period1 and Clock daily oscillations in transcript levels as revealed by ANOVA and cosinor analysis. Interestingly, expression profiles were inverted in retina and optic tectum compared to liver. Such differences suggest the existence of tissue-dependent zeitgebers for clock gene expression in this species (i.e., light for retina and optic tectum and feeding time for liver). This study provides novel insight into the location of the molecular clocks (central vs. peripheral) and their different phasing and synchronization pathways, which contributes to better understand the teleost circadian systems and its plasticity.

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Acknowledgments

This work was supported by grants from the Spanish Ministerio de Ciencia e Innovación (MICINN, AGL2007-66507-C02-01) and Junta de Andalucía (P06-AGR-01939) to José A. Muñoz-Cueto, and a predoctoral fellow of the Spanish MICINN (BES-2005-8629) to Águeda J. Martín-Robles. We thank Francesca Confente for her help in sampling. We also thank all staff from the “Planta de Cultivos Marinos” (University of Cádiz) for maintaining the animals used in these studies. Sequencing analyses were performed in Servicio Central de Ciencia y Tecnología (University of Cádiz). This is the contribution nº 1 from the CEIMAR Journal Publication Series.

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Authors and Affiliations

  1. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Campus de Excelencia Internacional del Mar (CEI MAR), 11510, Puerto Real, Spain

    Águeda J. Martín-Robles & José A. Muñoz-Cueto

  2. Departamento de Biomedicina, Biotecnología y Salud Pública, Facultad de Ciencias, Universidad de Cádiz, 11510, Puerto Real, Spain

    Águeda J. Martín-Robles & Carlos Pendón

  3. CACYTMAR, Institutos de Investigación, Campus Universitario de Puerto Real, 11510, Puerto Real, Spain

    Águeda J. Martín-Robles & José A. Muñoz-Cueto

  4. Department of Cell and Developmental Biology, Centre for Cell and Molecular Dynamics, University College of London, London, WC1E 6DE, UK

    David Whitmore

  5. Departamento de Fisiología, Facultad de Biología, Universidad de Murcia, 30100, Murcia, Spain

    Francisco Javier Sánchez-Vázquez

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  1. Águeda J. Martín-Robles
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  3. Francisco Javier Sánchez-Vázquez
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  5. José A. Muñoz-Cueto
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Correspondence to Carlos Pendón or José A. Muñoz-Cueto.

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Martín-Robles, Á.J., Whitmore, D., Sánchez-Vázquez, F.J. et al. Cloning, tissue expression pattern and daily rhythms of Period1, Period2, and Clock transcripts in the flatfish Senegalese sole, Solea senegalensis . J Comp Physiol B 182, 673–685 (2012). https://doi.org/10.1007/s00360-012-0653-z

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