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Effect of Nitric Oxide and Hydrogen Peroxide on Adventitious Root Development from Cuttings of Ground-Cover Chrysanthemum and Associated Biochemical Changes

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Abstract

It is well known that plant adventitious root formation can be stimulated by the application of nitric oxide (NO) and hydrogen peroxide (H2O2) exogenously but the mechanism of this physiological response is still unclear. Ground-cover chrysanthemum (Dendranthema morifolium ‘Beiguozhicun’) was used to understand the effects of NO and H2O2 on the rooting of plant cuttings and the associated biochemical changes of the rooting zone during the rhizogenesis process. The results showed that the effect of NO or H2O2 on rooting of ground-cover chrysanthemum cuttings was dose-dependent, with a maximal biological response at 50 μM of NO donor sodium nitroprusside (SNP) or 200 μM H2O2. There was a synergistic effect between NO and H2O2 on mediating rooting. NO and H2O2 treatments at the proper dosage might increase the activities of polyphenol oxidase (PPO) and indoleacetic acid oxidase (IAAO) and the content of water-soluble carbohydrate (WSC) and total nitrogen, while decreasing the total polyphenol content of ground-cover chrysanthemum cuttings. In addition, rooting percentage was significantly correlated with these biochemical constituent activities or contents. Together, these results indicated that NO and H2O2 treatments enhanced adventitious root development synergistically and independently by stimulating the activities of PPO and IAAO enzymes and the content of carbohydrate and nitrogen and simultaneously repressing the production of polyphenol.

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References

  • Bagatharia SB, Chanda SV (1998) Changes in peroxidase and IAA oxidase activities during cell elongation in Phaseolus hypocotyls. Acta Physiol Plant 20(1):9–13

    Article  CAS  Google Scholar 

  • Beffa R, Martin HV, Pilet PE (1990) In vitro oxidation of indoleacetic acid by soluble auxin-oxidases and peroxidases from maize roots. Plant Physiol 94:485–491

    Article  CAS  PubMed  Google Scholar 

  • Beligni MV, Lamattina L (2001) Nitric oxide: a nontraditional regulator of plant growth. Trends Plant Sci 6:508–509

    Article  CAS  PubMed  Google Scholar 

  • Berthon JY, Battraw MJ, Gaspar T, Boyer N (1993) Early test using phenolic compounds and peroxidase activity to improve in vitro rooting of Sequoiadendron giganteum (Lindl.). Buchholz Saussurea 27:7

    Google Scholar 

  • Bolduc RJ, Cherry JH, Blair BO (1970) Increase in indoleacetic acid-oxidase activity of winter wheat by cold treatment and gibberellic acid. Plant Physiol 45:461–464

    Article  CAS  PubMed  Google Scholar 

  • Bright J, Desikan R, Hancock JT, Weir IS, Neill SJ (2006) ABA-induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesis. Plant J 45:113–122

    Article  CAS  PubMed  Google Scholar 

  • Chandler SF, Dodds JH (1983) The effect of phosphate, nitrogen and sucrose in the production of phenolics and solasidine in callus cultures of Solanum laciniatum. Plant Cell Rep 2:105–108

    Article  Google Scholar 

  • Coban H (2007) Determination of polyphenol oxidase activity during rooting in cuttings of some grape varieties (Vitis vinifera L). Asian J Chem 19:4020–4024

    CAS  Google Scholar 

  • Correa-Aragunde NM, Graziano ML, Lamattina L (2004) Nitric oxide plays a central role in determining lateral root development in tomato. Planta 218:900–905

    Article  CAS  PubMed  Google Scholar 

  • De Klerk GJ (1996) Markers of adventitious root formation. Agronomie 16:609–616

    Article  Google Scholar 

  • Delledonne M, Xia YJ, Dixon RA, Lamb C (1998) Nitric oxide functions as a signal in plant disease resistance. Nature 394:585–588

    Article  CAS  PubMed  Google Scholar 

  • Desikan R, Griffiths R, Hancock J, Neill S (2002) A new role for an old enzyme: nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana. Proc Nat Acad Sci USA 99:16314–16318

    Article  CAS  PubMed  Google Scholar 

  • Druege U, Zerche S, Kadner R, Ernst M (2000) Relation between nitrogen status, carbohydrate distribution and subsequent rooting of chrysanthemum cuttings as affected by preharvest nitrogen supply and cold-storage. Ann Bot 85:687–701

    Article  CAS  Google Scholar 

  • Dunand C, Crèvecoeur M, Penel C (2007) Distribution of superoxide and hydrogen peroxide in Arabidopsis root and their influence on root development: possible interaction with peroxidases. New Phytol 174:332–341

    Article  CAS  PubMed  Google Scholar 

  • Ferrer AS, Bru R, Cabanes J, Carmona FG (1988) Characterization of catecholase and cresolase activities of monastrell grape polyphenol oxidase. Phytochemistry 27:319–321

    Article  Google Scholar 

  • Foreman J, Demidchik V, Bothwell JH, Mylona P, Miedema H, Torres MA, Linstead P, Costa S, Brownlee C, Jones JD, Davies JM, Dolan L (2003) Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. Nature 422:442–446

    Article  CAS  PubMed  Google Scholar 

  • Gouvěa CM, Souza CP, Magalhaes CA, Martin IS (1997) NO-releasing substances that induce growth elongation in maize root segments. Plant Growth Regul 21:183–187

    Article  Google Scholar 

  • Gove JP, Hoyle MC (1975) The isozymic similarity of indoleacetic acid oxidase to peroxidase in birch and horseradish. Plant Physiol 56:684–687

    Article  CAS  PubMed  Google Scholar 

  • Haissig BE, Davis TD, Riemenschneider DE (1992) Researching the controls of adventitious rooting. Physiol Plant 84:310–317

    Article  CAS  Google Scholar 

  • Hu X, Neill SJ, Tang Z, Cai W (2005) Nitric oxide mediates gravitropic bending in soybean roots. Plant Physiol 137:663–670

    Article  CAS  PubMed  Google Scholar 

  • Jàsik J, De Klerk GJ (1997) Anatomical and ultrastructural examination of adventitious root formation in stem slices of apple. Biol Plant 39:79–90

    Article  Google Scholar 

  • Joo JH, Bae YS, Lee JS (2001) Role of auxin-induced reactive oxygen species in root gravitropism. Plant Physiol 3:1055–1060

    Article  Google Scholar 

  • Knowles NR, Ries SK (1981) Rapid growth and apparent total nitrogen increases in rice and corn plants following applications of triacontanol. Plant Physiol 68:1279–1284

    Article  CAS  PubMed  Google Scholar 

  • Li M, Leung DWM (2000) Starch accumulation is associated with adventitious root formation in hypocotyl cuttings of Pinus radiata. J Plant Growth Regul 19:423–428

    CAS  Google Scholar 

  • Li SW, Xue LG, Xu SJ, Feng HY, An LZ (2007) Hydrogen peroxide involvement in formation and development of adventitious roots in cucumber. Plant Growth Regul 52:173–180

    Article  CAS  Google Scholar 

  • Liu ZH, Hsiao IC, Pan YW (1996) Effect of naphthaleneacetic acid on endogenous indole-3-acetic acid, peroxidase and auxin oxidase in hypocotyls cuttings of soybean during root formation. Bot Bull Acad Sin 37(4):247–253

    CAS  Google Scholar 

  • Lombardo MC, Graziano ML, Polacco JC, Lamattina L (2006) Nitric oxide functions as a positive regulator of root hair development. Plant Signal Behav 1:28–33

    PubMed  Google Scholar 

  • Neill S, Barros R, Bright J, Desikan R, Hancock J, Harrison J, Morris P, Ribeiro D, Wilson I (2008) Nitric oxide, stomatal closure, and abiotic stress. J Exp Bot 59(2):165–176

    Article  CAS  PubMed  Google Scholar 

  • Pagnussat GC, Simontacchi M, Puntarulo S, Lamattina L (2002) Nitric oxide is required for root organogenesis. Plant Physiol 129:954–956

    Article  CAS  PubMed  Google Scholar 

  • Pagnussat CG, Lanteri ML, Lombardo MC, Lamattina L (2004) Nitric oxide mediates the indole acetic acid induction activation of a mitogen-activated protein kinase cascade involved in adventitious root development. Plant Physiol 135:279–286

    Article  CAS  PubMed  Google Scholar 

  • Paul MJ, Driscoll SP, Lawlor DW (1991) The effect of cooling on photosynthesis, amounts of carbohydrate and assimilate export in sunflower. J Exp Bot 240:845–852

    Article  Google Scholar 

  • Pei ZM, Murata Y, Benning G, Thomine S, Klüsener B, Allen GJ, Grill E, Schroeder JI (2000) Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature 406:731–734

    Article  CAS  PubMed  Google Scholar 

  • Rentel MC, Lecourieux D, Ouaked F, Usher SL, Petersen L, Okamoto H, Knight H, Peck SC, Grierson CS, Hirt H, Knight MR (2004) OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis. Nature 427:858–861

    Article  CAS  PubMed  Google Scholar 

  • Rout GR (2006) Effect of auxins on adventitious root development from single node cuttings of Camellia sinensis (L.) Kuntze and associated biochemical changes. Plant Growth Regul 48:111–117

    Article  CAS  Google Scholar 

  • She XP, Song XG, He JM (2004) The role and relationship of nitric oxide and hydrogen peroxide in light/dark-regulated stomatal movement in Vicia faba. Acta Bot Sin 46:1292–1300

    CAS  Google Scholar 

  • Smart DR, Kocsis L, Walker MA, Stockert C (2003) Dormant buds and adventitious root formation by Vitis and other woody plants. J Plant Growth Regul 21:296–314

    Article  Google Scholar 

  • Su GX, Zhang WH, Liu YL (2006) Involvement of hydrogen peroxide generated by polyamine oxidative degradation in the development of lateral roots in soybean. J Integr Plant Biol 48:426–432

    Article  CAS  Google Scholar 

  • Van Handel E (1968) Direct microdetermination of sucrose. Anal Biochem 22:280–283

    Article  PubMed  Google Scholar 

  • Wang SY, Jiao HJ, Faust M (1991) Changes in the activities of catalase, peroxidase, and polyphenol oxidase in apple buds during bud break induced by thidiazuron. J Plant Growth Regul 10:33–39

    Article  Google Scholar 

  • Zhang A, Jiang M, Zhang J, Ding H, Xu S, Hu X, Tan M (2007) Nitric oxide induced by hydrogen peroxide mediates abscisic acid-induced activation of the mitogen-activated protein kinase cascade involved in antioxidant defense in maize leaves. New Phytol 175:36–50

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 40501076) and the Key Scientific and Technological Project of Lanzhou city, Lanzhou, China (No. 05-1-39; 07-1-04). The authors are grateful to the editors and the anonymous reviewers for their valuable comments and help.

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Authors and Affiliations

  1. Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, College of Agronomy, Gansu Agricultural University, Lanzhou, 730070, China

    Wei-Biao Liao

  2. Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730070, China

    Wei-Biao Liao & Hong-Lang Xiao

  3. College of Prataculture, Gansu Agricultural University, Lanzhou, 730070, China

    Mei-Ling Zhang

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  1. Wei-Biao Liao
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  2. Hong-Lang Xiao
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Correspondence to Mei-Ling Zhang.

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Liao, WB., Xiao, HL. & Zhang, ML. Effect of Nitric Oxide and Hydrogen Peroxide on Adventitious Root Development from Cuttings of Ground-Cover Chrysanthemum and Associated Biochemical Changes. J Plant Growth Regul 29, 338–348 (2010). https://doi.org/10.1007/s00344-010-9140-5

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  • DOI: https://doi.org/10.1007/s00344-010-9140-5

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