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A genetic linkage map of the vervet monkey (Chlorocebus aethiops sabaeus)

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Abstract

The spectacular progress in genomics increasingly highlights the importance of comparative biology in biomedical research. In particular, nonhuman primates, as model systems, provide a crucial intermediate between humans and mice. The close similarities between humans and other primates are stimulating primate studies in virtually every area of biomedical research, including development, anatomy, physiology, immunology, and behavior. The vervet monkey (Chlorocebus aethiops sabaeus) is an important model for studying human diseases and complex traits, especially behavior. We have developed a vervet genetic linkage map to enable mapping complex traits in this model organism and facilitate comparative genomic analysis between vervet and other primates. Here we report construction of an initial genetic map built with about 360 human orthologous short tandem repeats (STRs) that were genotyped in 434 members of an extended vervet pedigree. The map includes 226 markers mapped in a unique order with a resolution of 9.8 Kosambi centimorgans (cM) in the vervet monkey genome, and with a total length (including all 360 markers) of 2726 cM. At least one complex and 11 simple rearrangements in marker order distinguish vervet chromosomes from human homologs. While inversions and insertions can explain a similar number of changes in marker order between vervet and rhesus homologs, mostly inversions are observed when vervet chromosome organization is compared to that in human and chimpanzee. Our results support the notion that large inversions played a less prominent role in the evolution within the group of the Old World monkeys compared to the human and chimpanzee lineages.

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Acknowledgments

The authors thank Dr. Stephen Sawcer (Genetic Analysis of Multiple Sclerosis in Europeans Initiative, Cambridge, UK) for providing fluorescently labeled human markers and Maricel Almonte and Harpreet Sandhu for their contribution in genotyping. This work was supported by NIH grant R01 RR016300 (NBF) and funding from Genome Canada and Genome Québec (KD). The Center for Primate Neuroethology, which supplied the pedigree samples, was supported by NIH grants MH061852 and RR019963 (LAF).

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Authors and Affiliations

  1. Center for Neurobehavioral Genetics, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, California, 90095, USA

    Anna J. Jasinska, Susan Service, Matthew Levinson, Erin Slaten, Oliver Lee, Nelson B. Freimer & Roel A. Ophoff

  2. Department of Human Genetics, University of California, Los Angeles, California, 90095, USA

    Eric Sobel & Roel A. Ophoff

  3. Center for Primate Neuroethology, The Jane and Terry Semel Institute for Neuroscience and Human Behavior University of California, Los Angeles, California, 90095, USA

    Lynn A. Fairbanks, Julia N. Bailey, Matthew J. Jorgensen & Sherry E. Breidenthal

  4. Department of Psychiatry and Biobehavioral Sciences, University of California, Los Angeles, California, 90095, USA

    Lynn A. Fairbanks & Roel A. Ophoff

  5. Research Institute of McGill University Health Centre, McGill University and Genome Québec Innovation Centre, and Departments of Human Genetics and Experimental Medicine, McGill University, Montréal, Québec, H3A 1A1, Canada

    Ken Dewar & Thomas J. Hudson

  6. Ontario Institute for Cancer Research, 101 College Street, Toronto, Ontario, M5G 1L7, Canada

    Thomas J. Hudson

  7. Departments of Psychiatry and Human Genetics, McGill University, Montréal, H3A 1A1, Québec, Canada

    Roberta Palmour

  8. Department of Medical Genetics and Rudolf Magnus Institute, University Medical Center Utrecht, Universiteitsweg 100, 3584CG, Utrecht, The Netherlands

    Roel A. Ophoff

  9. UCLA Center for Neurobehavioral Genetics, Gonda Center, Rm. 3506, 695 Charles E. Young Dr. S., Box 951761, Los Angeles, CA, 90095, USA

    Nelson B. Freimer

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  1. Anna J. Jasinska
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  2. Susan Service
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  3. Matthew Levinson
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  5. Oliver Lee
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  7. Lynn A. Fairbanks
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  8. Julia N. Bailey
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Correspondence to Nelson B. Freimer.

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Jasinska, A.J., Service, S., Levinson, M. et al. A genetic linkage map of the vervet monkey (Chlorocebus aethiops sabaeus). Mamm Genome 18, 347–360 (2007). https://doi.org/10.1007/s00335-007-9026-4

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  • DOI: https://doi.org/10.1007/s00335-007-9026-4

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