Abstract
Five known thymol derivatives were isolated from roots of Arnica montana transformed with Agrobacterium rhizogenes LBA 9402. The compounds were characterized by spectral methods. The pattern of thymol derivatives in light-grown hairy roots was slightly different from that in dark-grown ones. This is the first report on the presence of thymol derivatives in hairy roots of the plant.
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Bohlmann F, Zdero C (1972) Neue Thymol-Derivate aus Arnica amplexicaulis. Tetrahedron Lett 28:2827–2828
Bohlmann F, Niedballa U, Schülz J (1969) Über einige Thymolderivative aus Gaillardia- und Helenium-Arten. Chem Ber 102:864–871
Bohlmann F, Jakupovic J, Lonitz M (1977) Uber Inhaltsstoffe der Eupatorium—Gruppe. Chem Ber 110:301–314
Bohlmann F, Jakupovic J, King RM, Robinson H (1982) New labdane derivatives from Madia sativa. Phytochemistry 21:1103–1107
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
Gonzalez A, Barrera JB, Rosas FE, Hernandez ACY, Espineira J, Joseph-Nathan P (1986) Thymol derivatives from Schizogyne glaberrima. Phytochemistry 25:2889–2891
Herz W, Sosa E (1988) Sesquiterpene lactones and other constituents of Arnica acaulis. Phytochemistry 27:155–159
Hosokawa K, Matsuki R, Oikawa Y, Yamamura S (1997) Genetic transformation of gentian using wild-type Agrobacterium rhizogenes. Plant Cell Tissue Organ Cult 51:137–140
Maldonado E, Marquez CL, Ortega A (1992) A thymol derivative from Calea nelsonii. Phytochemistry 31:2527–2528
Metwally MA, Dawidar AM (1985) A thymol derivative from Inula crithmoides. Phytochemistry 24:1377–1378
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Passreiter CM, Matthiesen U, Willuhn G (1998) 10-Acetoxy-9-chloro-8,9-dehydrothymol and further thymol derivatives from Arnica sachalinensis. Phytochemistry 49:777–781
Petit A, David C, Dahl JG, Ellis P, Guyon P, Casse-Delbard F, Tempe J (1983) Further extension of opine concept: plasmids in Agrobacterium rhizogenes cooperate for opine degradation. Mol Gen Genet 190:2004–2014
Rossetti V, Lombard A, Sancin P, Buffa M, Di Stefano R (1984) Characterization of Arnica montana L. dried roots. Int J Crude Drug Res 22:53–60
Su BN, Takaishi Y, Yabuuchi T, Kusumi T, Tori M, Takaoka S, Honda G, Ito M, Takeda Y, Kodzhimatov OK, Ashurmetov O (2001) Sesquiterpenes and monoterpenes from bark of Inula macrophylla. J Nat Prod 64:466–471
Tori M, Ohara Y, Nakashima K, Sono M (2001) Thymol derivatives from Eupatorium fortunei. J Nat Prod 64:1048–1051
Vervliet G, Holsters M, Teuchy H, Montagi M, Schell J (1975) Characterization of different plaque-forming and defective temperate pharges in Agrobacterium strains. J Gen Virol 26:33–48
Wagner S, Suter A, Merfort I (2004) Skin penetration studies of Arnica preparations and of their sesquiterpene lactones. Planta Med 70:897–903
Weremczuk-Jeżyna I, Wysokińska H (2004) Kultury korzeni transformowanych Arnica montana—izolacja metabolitów wtórnych. Biotechnologia 2:54–60
Willuhn G (1972) Untersuchungen über die Inhaltsstoffe von Arnica—Arten. VII. Die Zusammensetzung des ätherischen Öles aus den unterirdischen Organen und Blütenkörbchen verschiedener Arnica—Arten. Planta Med 22:1–33
Willuhn G (1998) Arnica flowers: pharmacology, toxicology and analysis of the sesquiterpene lactones—their main active substances. ACS Symp Ser 691 Am Chem Soc 118–132
Zee OP, Kim DK, Lee KR (1998) Thymol derivatives from Carpesium divaricatum. Arch Pharm Res 21:618–620
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This investigation was supported by a research grant 503-312-1 from Medical University of Łódź.
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Communicated by H. Van Onckelen
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Weremczuk-Jeżyna, I., Kisiel, W. & Wysokińska, H. Thymol derivatives from hairy roots of Arnica montana . Plant Cell Rep 25, 993–996 (2006). https://doi.org/10.1007/s00299-006-0157-y
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DOI: https://doi.org/10.1007/s00299-006-0157-y