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Transformation of apple (Malus domestica Borkh.) with the stilbene synthase gene from grapevine (Vitis vinifera L.) and a PGIP gene from kiwi (Actinidia deliciosa)

  • Genetic Transformation and Hybridization
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Abstract

The objective of the present research was to introduce genes with antifungal potential into the commercially important apple cvs. Elstar and Holsteiner Cox in order to establish resistance against fungal diseases. The gene encoding the stilbene synthase (Vst1) from Vitis vinifera L., responsible for the synthesis of the phytoalexin resveratrol in grapevine, and the gene for a polygalacturonase-inhibiting protein (PGIP) from kiwi (Actinidia deliciosa) were transferred into Holsteiner Cox and Elstar via Agrobacterium tumefaciens-mediated transformation. A total of nine transgenic Holsteiner Cox clones and one transgenic E clone carrying the stilbene-synthase gene as well as three transgenic Holsteiner Cox lines harbouring the polygalacturonase-inhibiting protein from Kiwi were identified via polymerase chain reaction and Southern blot analysis. High performance liquid chromatography analysis revealed the accumulation of a resveratrol-derivate, a glycoside, in transgenic Vst1 plants.

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Fig. 1a, b.
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Fig. 7A, B.
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Abbreviations

BAP :

6-Benzylaminopurine

E :

Elstar

H :

Holsteiner Cox

HPLC :

High performance liquid chromatography

IBA :

Indole-3-butyric acid

NAA :

α-Naphthaleneacetic acid

TDZ :

Thidiazuron

YEP :

Yeast extract broth

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Acknowledgements

We thank Rüdiger Hain, Bayer AG, for providing the Vst1 gene and the Scottish Research Center for providing PSCP2. Elstar in vitro cultures were kindly provided by Anette Urbanietz, BAZ Ahrensburg.

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Authors and Affiliations

  1. Abteilung Obstbau, Institut für Gemüse- und Obstbau, Universität Hannover, Am Steinberg 3, 31157, Sarstedt, Germany

    I. Szankowski & J. Schönherr

  2. Lehrgebiet Molekulargenetik, Universität Hannover, Herrenhaeuserstr. 2, 30419, Hannover, Germany

    H-J. Jacobsen & H. Kiesecker

  3. Bundesforschungsanstalt für Ernährung, Institut für Ernährungsphysiologie, Haid- und Neustr. 9, 76131, Karlsruhe, Germany

    K. Briviba & J. Fleschhut

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  1. I. Szankowski
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  2. K. Briviba
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  3. J. Fleschhut
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  4. J. Schönherr
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  5. H-J. Jacobsen
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  6. H. Kiesecker
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Correspondence to I. Szankowski.

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Communicated by H. Lörz

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Szankowski, I., Briviba, K., Fleschhut, J. et al. Transformation of apple (Malus domestica Borkh.) with the stilbene synthase gene from grapevine (Vitis vinifera L.) and a PGIP gene from kiwi (Actinidia deliciosa). Plant Cell Rep 22, 141–149 (2003). https://doi.org/10.1007/s00299-003-0668-8

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  • DOI: https://doi.org/10.1007/s00299-003-0668-8

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