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Mutations affecting spindle pole body and mitotic exit network function are synthetically lethal with a deletion of the nucleoporin NUP1 in S. cerevisiae

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Abstract

Nuclear pore complexes (NPCs) are embedded in the nuclear envelope of eukaryotic cells and function to regulate passage of macromolecules in and out of the nucleus. Nup1 is one of 30 nucleoporins comprising the NPC of the yeast Saccharomyces cerevisiae and is located on the nucleoplasmic face of the NPC where it plays a role in mRNA export and protein transport. In order to further characterize the function of Nup1 we used a genetic approach to identify mutations that are synthetically lethal in combination with a deletion of NUP1 (nup1Δ). We have identified one such nup1 lethal mutant (nle6) as a temperature sensitive allele of nud1. NUD1 encodes a component of the yeast spindle pole body (SPB) and acts as scaffolding for the mitotic exit network (MEN). We observe that nle6/nud1 mutant cells have a normal distribution of NPCs within the nuclear envelope and exhibit normal rates of nuclear protein import at both the permissive and restrictive temperatures. nup1Δ also exhibits synthetic lethality with bub2Δ and bfa1Δ, both of which encode proteins that colocalize with Nud1 at spindle pole bodies and function in the mitotic exit network. However, we do not observe genetic interactions among nle6/nud1, bub2Δ, or bfa1Δ and mutations in the nucleoporin encoding genes NUP60 or NUP170, nor is nup1Δ synthetically lethal with the absence of components downstream in the mitotic exit network, including Lte1, Swi5, and Dbf2. Our results suggest a novel functional connection between Nup1 and proteins comprising both the spindle pole body and early mitotic exit network.

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Acknowledgments

The authors acknowledge S. Wente, J. Kilmartin, E. Schiebel, R. Li, N. Shulga, and D. Goldfarb for their generous sharing of yeast strains and plasmids. They also thank S. Geier, M. Gordon, and L. Parris for technical assistance and K. G. Belanger, M. Pettit, and K. Kokanovich for critical reading of this manuscript. Support for this work was provided by National Institutes of Health grant GM-65107 to K.D.B., Colgate summer undergraduate research fellowships to N.C.H. and N.T.A-G., and funding from the Howard Hughes Medical Institute in support of Colgate’s off-campus undergraduate research program at the NIH. M.B. was supported by funds from the Intramural Research Program of the NIH and NCI.

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  1. Nicola C. Harper

    Present address: Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA, 94720, USA

  2. Nicole T. Al-Greene

    Present address: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA

Authors and Affiliations

  1. Department of Biology, Colgate University, 13 Oak Dr., Hamilton, NY, 13346, USA

    Nicola C. Harper, Nicole T. Al-Greene & Kenneth D. Belanger

  2. Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20889, USA

    Munira A. Basrai

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  1. Nicola C. Harper
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Correspondence to Kenneth D. Belanger.

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Communicated by K. Kuchler.

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Harper, N.C., Al-Greene, N.T., Basrai, M.A. et al. Mutations affecting spindle pole body and mitotic exit network function are synthetically lethal with a deletion of the nucleoporin NUP1 in S. cerevisiae . Curr Genet 53, 95–105 (2008). https://doi.org/10.1007/s00294-007-0168-4

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