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Carbon Catabolite Control is Important for Listeria monocytogenes Biofilm Formation in Response to Nutrient Availability

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Abstract

The foodborne pathogen Listeria monocytogenes has the ability to develop biofilm in food-processing environment, which becomes a major concern for the food safety. The biofilm formation is strongly influenced by the availability of nutrients and environmental conditions, and particularly enhanced in poor minimal essential medium (MEM) containing glucose rather than in rich brain heart infusion (BHI) broth. To gain better insight into the conserved protein expression profile in these biofilms, the proteomes from biofilm- and planktonic-grown cells from MEM with 50 mM glucose or BHI were compared using two-dimensional polyacrylamide gel electrophoresis followed by MALDI-TOF/TOF analysis. 47 proteins were successfully identified to be either up (19 proteins) or down (28 proteins) regulated in the biofilm states. Most (30 proteins) of them were assigned to the metabolism functional category in cluster of orthologous groups of proteins. Among them, up-regulated proteins were mainly associated with the pentose phosphate pathway and glycolysis, whereas a key enzyme CitC involved in tricarboxylic acid cycle was down-regulated in biofilms compared to the planktonic states. These data implicate the importance of carbon catabolite control for L. monocytogenes biofilm formation in response to nutrient availability.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (30970111), Excellent Youth Foundation of Hubei Scientific Committee (No. 2009CDA124) and CCNU Project (No. CCNU09Y01001).

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Authors and Affiliations

  1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Luo Yu Road 152, 430079, Wuhan, People’s Republic of China

    Qingchun Zhou, Xiaoqin Feng, Qiang Zhang, Feifei Feng, Xiaojiao Yin, Junli Shang, Huiping Qu & Qin Luo

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  1. Qingchun Zhou
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  2. Xiaoqin Feng
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  3. Qiang Zhang
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  4. Feifei Feng
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  7. Huiping Qu
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  8. Qin Luo
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Correspondence to Qin Luo.

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Qingchun Zhou and Xiaoqin Feng contributed equally to this work and should be considered as co-first authors.

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Zhou, Q., Feng, X., Zhang, Q. et al. Carbon Catabolite Control is Important for Listeria monocytogenes Biofilm Formation in Response to Nutrient Availability. Curr Microbiol 65, 35–43 (2012). https://doi.org/10.1007/s00284-012-0125-4

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  • DOI: https://doi.org/10.1007/s00284-012-0125-4

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