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A Novel Gene Cluster soxSRT Is Essential for the Chemolithotrophic Oxidation of Thiosulfate and Tetrathionate by Pseudaminobacter salicylatoxidans KCT001

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Abstract

Chemolithotrophic sulfur oxidation (Sox) in the α-proteobacterium Pseudaminobacter salicylatoxidans KCT001 was found to be governed by the gene cluster soxSRT–soxVWXYZABCD. Independent transposon-insertion mutations in the genes soxB, soxC, soxD, and also in a novel open reading frame (ORF), designated as soxT, afforded revelation of the entire sox locus of this bacterium. The deduced amino acid sequence of the novel ORF soxT comprised 362 residues and exhibited significant homology with hypothetical proteins of diverse origin, including a permease-like transport protein of Escherichia coli. Two contiguous ORFs, soxR and soxS, immediately preceded the soxT gene. The gene cluster soxSRT was located upstream of soxVWXYZABCD and was transcribed divergently with respect to the latter. Chemolithotrophic utilization of both thiosulfate and tetrathionate was observed to have been impaired in all of these Sox− mutants, implicating the involvement of the gene cluster soxSRT–soxVWXYZABCD in the oxidation of both thiosulfate and tetrathionate.

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Acknowledgments

The assistance provided by Biswanath De Sarkar and Nityananda Patra is acknowledged. This work was supported by the Council of Scientific and Industrial Research, Government of India, Extramural Research Unit-II, under Project No. 37/1091/02-EMR-II. S. M. and B. D. were supported by C. S. I. R. fellowships.

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Authors and Affiliations

  1. Department of Microbiology, Bose Institute, P-1/12, C. I. T. Scheme VII-M, Kolkata, 700 054, India

    Chandrajit Lahiri, Sukhendu Mandal, Wriddhiman Ghosh, Bomba Dam & Pradosh Roy

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  1. Chandrajit Lahiri
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  2. Sukhendu Mandal
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  3. Wriddhiman Ghosh
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  4. Bomba Dam
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  5. Pradosh Roy
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Correspondence to Sukhendu Mandal.

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Pradosh Roy Deceased

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Lahiri, C., Mandal, S., Ghosh, W. et al. A Novel Gene Cluster soxSRT Is Essential for the Chemolithotrophic Oxidation of Thiosulfate and Tetrathionate by Pseudaminobacter salicylatoxidans KCT001. Curr Microbiol 52, 267–273 (2006). https://doi.org/10.1007/s00284-005-0176-x

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  • DOI: https://doi.org/10.1007/s00284-005-0176-x

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