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Composition and dynamics of microbial community in a zeolite biofilter-membrane bioreactor treating coking wastewater

  • Environmental biotechnology
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Abstract

In this study, a lab-scale anaerobic/anoxic/zeolite biofilter-membrane bioreactor (A1/A2/ZB-MBR) was designed to treat coking wastewater. The 454 pyrosequencing was used to obtain the composition and dynamics of microbial community about the treatment system. The results showed that the system yielded stable effluent chemical oxidation demand (158.5 ± 21.8 mg/L) and ammonia (8.56 ± 7.30 mg/L), but fluctuant total nitrogen (31.4–165.1 mg/L) concentrations. In addition, 66,256 16S rRNA gene sequences were obtained from A2 and ZB-MBR, and the microbial diversity and richness for five samples were determined. Although community compositions in the five samples were quite different, bacteria assigned to phylum Proteobacteria and class Flavobacteria commonly existed and dominated the microbial populations. The pyrosequencing analysis revealed that the microbial community shifted in the ZB-MBR with the presence of zeolite. Some taxa began to appear in ZB-MBR and contributed to the system performance. Additionally, Nitrosomonas and Nitrobacter gradually became the dominant ammonia-oxidizing bacteria and nitrite-oxidizing bacteria during the operation, respectively, which are favorable for the stabilized ammonia removal. Our results proved that the ZB-MBR is an alternative technique for treating coking wastewater.

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References

  • Adebusoye SA, Ilori MO, Picardal FW, Amund OO (2008) Cometabolic degradation of polychlorinated biphenyls (PCBs) by axenic cultures of Ralstonia sp strain SA–5 and Pseudomonas sp strain SA–6 obtained from Nigerian contaminated soils. World J Microbiol Biotechnol 24(1):61–68

    Article  CAS  Google Scholar 

  • APHA (2005) Standard methods for the examination of water and wastewater, 21st edn. American Public Health Association/American Water Works Association/Water Environmental Federation, Washington

    Google Scholar 

  • Arellano-Garcia L, Revah S, Ramirez M, Gomez JM, Cantero D (2009) Dimethyl sulphide degradation using immobilized Thiobacillus thioparus in a biotrickling filter. Environ Technol 30(12):1273–1279

    Article  PubMed  CAS  Google Scholar 

  • Bai YH, Shi Q, Wen DH, Li ZX, Jefferson WA, Feng CP, Tang XY (2012) Bacterial communities in the sediments of Dianchi Lake, a partitioned eutrophic waterbody in China. PLoS One 7(5):e37796

    Article  PubMed  CAS  Google Scholar 

  • Bai YH, Sun QH, Xing R, Wen DH, Tang XY (2011a) Analysis of denitrifier community in a bioaugmented sequencing batch reactor for the treatment of coking wastewater containing pyridine and quinoline. Appl Microbiol Biotechnol 90(4):1485–1492

    Article  PubMed  CAS  Google Scholar 

  • Bai YH, Sun QH, Sun RH, Wen DH, Tang XY (2011b) Bioaugmentation and adsorption treatment of coking wastewater containing pyridine and quinoline using zeolite-biological aerated filters. Environ Sci Technol 45(5):1940–1948

    Article  PubMed  CAS  Google Scholar 

  • Bai YH, Sun QH, Xing R, Wen DH, Tang XY (2010) Removal of pyridine and quinoline by bio-zeolite composed of mixed degrading bacteria and modified zeolite. J Hazard Mater 181(1–3):916–922

    Article  PubMed  CAS  Google Scholar 

  • Bai YH, Sun QH, Zhao C, Wen DH, Tang XY (2009) Aerobic degradation of pyridine by a new bacterial strain, Shinella zoogloeoides BC026. J Ind Microbiol Biotechnol 36(11):1391–1400

    Article  PubMed  CAS  Google Scholar 

  • Blackburne R, Vadivelu VM, Yuan ZG, Keller J (2007) Kinetic characterisation of an enriched Nitrospira culture with comparison to Nitrobacter. Water Res 41(14):3033–3042

    Article  PubMed  CAS  Google Scholar 

  • Figuerola ELM, Erijman L (2007) Bacterial taxa abundance pattern in an industrial wastewater treatment system determined by the full rRNA cycle approach. Environ Microbiol 9(7):1780–1789

    Article  PubMed  CAS  Google Scholar 

  • Figuerola ELM, Erijman L (2010) Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification. J Hazard Mater 181(1–3):281–288

    Article  PubMed  CAS  Google Scholar 

  • Ha PT, Lee TK, Rittmann BE, Park J, Chang IS (2012) Treatment of alcohol distillery wastewater using a Bacteroidetes-dominant thermophilic microbial fuel cell. Environ Sci Technol 46(5):3022–3030

    Article  PubMed  CAS  Google Scholar 

  • Huson DH, Auch AF, Qi J, Schuster SC (2007) MEGAN analysis of metagenomic data. Genome Res 17(3):377–386

    Article  PubMed  CAS  Google Scholar 

  • Jin T, Zhang T, Yan QM (2010) Characterization and quantification of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in a nitrogen-removing reactor using T-RFLP and qPCR. Appl Microbiol Biotechnol 87(3):1167–1176

    Article  PubMed  CAS  Google Scholar 

  • Jones SE, Lennon JT (2010) Dormancy contributes to the maintenance of microbial diversity. PNAS 107(13):5881–5886

    Article  PubMed  CAS  Google Scholar 

  • Jorgensen TC, Weatherley LR (2003) Ammonia removal from wastewater by ion exchange in the presence of organic contaminants. Water Res 37(8):1723–1728

    Article  PubMed  CAS  Google Scholar 

  • Kim YM, Cho HU, Lee DS, Park D, Park JM (2011a) Influence of operational parameters on nitrogen removal efficiency and microbial communities in a full-scale activated sludge process. Water Res 45(17):5785–5795

    Article  PubMed  CAS  Google Scholar 

  • Kim YM, Lee DS, Park C, Park D, Park JM (2011b) Effects of free cyanide on microbial communities and biological carbon and nitrogen removal performance in the industrial activated sludge process. Water Res 45(3):1267–1279

    Article  PubMed  CAS  Google Scholar 

  • Kim YM, Cho HU, Lee DS, Park C, Park D, Park JM (2011c) Response of nitrifying bacterial communities to the increased thiocyanate concentration in pre-denitrification process. Bioresour Technol 102(2):913–922

    Article  PubMed  CAS  Google Scholar 

  • Lee TK, Doan TV, Yoo K, Choi S, Kim C, Park J (2010) Discovery of commonly existing anode biofilm microbes in two different wastewater treatment MFCs using FLX Titanium pyrosequencing. Appl Microbiol Biotechnol 87(6):2335–2343

    Article  PubMed  CAS  Google Scholar 

  • Li HB, Cao HB, Li YP, Zhang Y, Liu HR (2010) Innovative biological process for treatment of coking wastewater. Environ Eng Sci 27(4):313–322

    Article  CAS  Google Scholar 

  • Maranon E, Vazquez I, Rodriguez J, Castrillon L, Fernandez Y, Lopez H (2008) Treatment of coke wastewater in a sequential batch reactor (SBR) at pilot plant scale. Bioresour Technol 99(10):4192–4198

    Article  PubMed  CAS  Google Scholar 

  • McLellan SL, Huse SM, Mueller-Spitz SR, Andreishcheva EN, Sogin ML (2010) Diversity and population structure of sewage-derived microorganisms in wastewater treatment plant influent. Environ Microbiol 12(2):378–392

    Article  PubMed  CAS  Google Scholar 

  • Park S, Yu J, Byun I, Cho S, Park T, Lee T (2011) Microbial community structure and dynamics in a mixotrophic nitrogen removal process using recycled spent caustic under different loading conditions. Bioresour Technol 102(15):7265–7271

    Article  PubMed  CAS  Google Scholar 

  • Roldan MD, Blasco R, Caballero FJ, Castillo F (1998) Degradation of p-nitrophenol by the phototrophic bacterium Rhodobacter capsulatus. Arch Microbiol 169(1):36–42

    PubMed  CAS  Google Scholar 

  • Sanapareddy N, Hamp TJ, Gonzalez LC, Hilger HA, Fodor AA, Clinton SM (2009) Molecular diversity of a North Carolina wastewater treatment plant as revealed by pyrosequencing. Appl Environ Microbiol 75(6):1688–1696

    Article  PubMed  CAS  Google Scholar 

  • Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75(23):7537–7541

    Article  PubMed  CAS  Google Scholar 

  • Schmidt I, Sliekers O, Schmid M, Bock E, Fuerst J, Kuenen JG, Jetten MSM, Strous M (2003) New concepts of microbial treatment processes for the nitrogen removal in wastewater. FEMS Microbiol Rev 27(4):481–492

    Article  PubMed  CAS  Google Scholar 

  • Silva CC, Hayden H, Sawbridge T, Mele P, Kruger RH, Rodrigues MVN, Costa GGL, Vidal RO, Sousa MP, Torres APR, Santiago VMJ, Oliveira VM (2012) Phylogenetic and functional diversity of metagenomic libraries of phenol degrading sludge from petroleum refinery wastewater treatment system. AMB Express 2(1):1–13

    Article  Google Scholar 

  • Siripong S, Rittmann BE (2007) Diversity study of nitrifying bacteria in full-scale municipal wastewater treatment plants. Water Res 41(5):1110–1120

    Article  PubMed  CAS  Google Scholar 

  • Sueoka K, Satoh H, Onuki M, Mino T (2009) Microorganisms involved in anaerobic phenol degradation in the treatment of synthetic coke-oven wastewater detected by RNA stable-isotope probing. FEMS Microbiol Lett 291(2):169–174

    Article  PubMed  CAS  Google Scholar 

  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S Ribosomal DNA amplification for phylogenetic study. J Bacteriol 173(2):697–703

    PubMed  CAS  Google Scholar 

  • Wicker T, Schlagenhauf E, Graner A, Close TJ, Keller B, Stein N (2006) 454 sequencing put to the test using the complex genome of barley. Bmc Genomics 7:275

    Article  PubMed  Google Scholar 

  • Wilson MV, Shmida A (1984) Measuring beta diversity with presence absence data. J Ecol 72(3):1055–1064

    Article  Google Scholar 

  • Ye L, Shao M-F, Zhang T, Tong AHY, Lok S (2011) Analysis of the bacterial community in a laboratory-scale nitrification reactor and a wastewater treatment plant by 454-pyrosequencing. Water Res 45(15):4390–4398

    Article  PubMed  CAS  Google Scholar 

  • Yin N, Yang G, Zhong ZX, Xing WH (2011) Separation of ammonium salts from coking wastewater with nanofiltration combined with diafiltration. Desalination 268(1–3):233–237

    Article  CAS  Google Scholar 

  • Zhang H, Banaszak JE, Parameswaran P, Alder J, Krajmalnik-Brown R, Rittmann BE (2009) Focused-pulsed sludge pre-treatment increases the bacterial diversity and relative abundance of acetoclastic methanogens in a full-scale anaerobic digester. Water Res 43(18):4517–4526

    Article  PubMed  CAS  Google Scholar 

  • Zhang XJ, Yue SQ, Zhong HH, Hua WY, Chen RJ, Cao YF, Zhao LP (2011a) A diverse bacterial community in an anoxic quinoline-degrading bioreactor determined by using pyrosequencing and clone library analysis. Appl Microbiol Biotechnol 91(2):425–434

    Article  PubMed  CAS  Google Scholar 

  • Zhang LL, Leng SQ, Zhu RY, Chen JM (2011b) Degradation of chlorobenzene by strain Ralstonia pickettii L2 isolated from a biotrickling filter treating a chlorobenzene-contaminated gas stream. Appl Microbiol Biotechnol 91(2):407–415

    Article  PubMed  CAS  Google Scholar 

  • Zhang LL, Sun YH, Guo DL, Wu ZR, Jiang DM (2012) Molecular diversity of bacterial community of dye wastewater in an anaerobic sequencing batch reactor. Afr J Microbiol Res 6(35):6444–6453

    Article  CAS  Google Scholar 

  • Zhang T, Ye L, Tong AHY, Shao MF, Lok S (2011c) Ammonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors. Appl Microbiol Biotechnol 91(4):1215–1225

    Article  PubMed  CAS  Google Scholar 

  • Zhao WT, Huang X, Lee DJ, Wang XH, Shen YX (2009a) Use of submerged anaerobic–anoxic–oxic membrane bioreactor to treat highly toxic coke wastewater with complete sludge retention. J Membr Sci 330(1–2):57–64

    Article  CAS  Google Scholar 

  • Zhao WT, Huang X, Lee DJ (2009b) Enhanced treatment of coke plant wastewater using an anaerobic–anoxic–oxic membrane bioreactor system. Sep Purif Technol 66(2):279–286

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by an “863” Exploration Project (no. 2009AA06Z309) granted by the Ministry of Science and Technology of China.

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Authors and Affiliations

  1. School of Environment, Tsinghua University, Beijing, 100084, People’s Republic of China

    Xiaobiao Zhu, Jinping Tian, Cong Liu & Lujun Chen

  2. Key Lab of Water Science and Technology of Zhejiang Province, Jiaxing, 314050, People’s Republic of China

    Lujun Chen

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  1. Xiaobiao Zhu
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  2. Jinping Tian
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  3. Cong Liu
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  4. Lujun Chen
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Correspondence to Lujun Chen.

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Zhu, X., Tian, J., Liu, C. et al. Composition and dynamics of microbial community in a zeolite biofilter-membrane bioreactor treating coking wastewater. Appl Microbiol Biotechnol 97, 8767–8775 (2013). https://doi.org/10.1007/s00253-012-4558-2

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  • DOI: https://doi.org/10.1007/s00253-012-4558-2

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