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Yeast hydrolysate as a low-cost additive to serum-free medium for the production of human thrombopoietin in suspension cultures of Chinese hamster ovary cells

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Abstract

To enhance the performance of a serum-free medium (SFM) for human thrombopoietin (hTPO) production in suspension cultures of recombinant Chinese hamster ovary (rCHO) cells, several low-cost hydrolysates such as yeast hydrolysate (YH), soy hydrolysate, wheat gluten hydrolysate and rice hydrolysate were tested as medium additives. Among various hydrolysates tested, the positive effect of YH on hTPO production was most significant. When 5 g l–1 YH was added to SFM, the maximum hTPO concentration in batch culture was 40.41 μg ml–1, which is 11.5 times higher than that in SFM without YH supplementation. This enhanced hTPO production in YH-supplemented SFM was obtained by the combined effect of enhanced q hTPO (the specific rate of hTPO production). The supplementation of YH in SFM increased q hTPO by 294% and extended culture longevity by >2 days if the culture was terminated at a cell viability of 50%. Furthermore, cell viability throughout the culture using YH-supplemented SFM was higher than that using any other hydrolysate-supplemented SFM tested, thereby minimizing degradation of hTPO susceptible to proteolytic degradation. In addition, YH supplementation did not affect in vivo biological activity of hTPO. Taken together, the results obtained demonstrate the potential of YH as a medium additive for hTPO production in serum-free suspension cultures of rCHO cells.

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Acknowledgements

The authors thank Quest International for providing different kinds of hydrolysates and Ms. Sang Lim Choi for amino acid analysis. This research was supported in part by grants from the Ministry of Science and Technology (National Research Laboratory Program) and the Ministry of Education (Brain Korea 21 Program).

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Authors and Affiliations

  1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373–1 Kusong-Dong, Yusong-Gu, 305–701, Daejon , Korea

    Y. H. Sung, J. Y. Chung & G. M. Lee

  2. Daewoong, San 84–1 Samgye-Ri, Pogok-Myun, Yongin, 449–814, Kyunggi-Do, Korea

    S. W. Lim & J. Y. Chung

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  1. Y. H. Sung
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  2. S. W. Lim
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  4. G. M. Lee
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Correspondence to G. M. Lee.

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Sung, Y.H., Lim, S.W., Chung, J.Y. et al. Yeast hydrolysate as a low-cost additive to serum-free medium for the production of human thrombopoietin in suspension cultures of Chinese hamster ovary cells. Appl Microbiol Biotechnol 63, 527–536 (2004). https://doi.org/10.1007/s00253-003-1389-1

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  • DOI: https://doi.org/10.1007/s00253-003-1389-1

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