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Isolation and cDNA cloning of novel hydrogen peroxide-dependent phenol oxidase from the basidiomycete Termitomyces albuminosus

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Abstract

A novel hydrogen peroxide-dependent phenol oxidase (TAP) was isolated from the basidiomycete Termitomyces albuminosus. TAP is an extracellular monomeric enzyme with an estimated molecular weight of 67 kDa. The purified enzyme can oxidize various phenolic compounds in the presence of hydrogen peroxide, but cannot oxidize 3,4-dimethoxybenzyl (veratryl) alcohol. MnII was not required for catalysis by TAP. The optimum pH for TAP activity was 2.3, which is the lowest known optimum pH for a fungal phenol oxidase. The cDNA encoding TAP was cloned with reverse transcription-polymerase chain reaction (RT-PCR) using degenerate primers based on the N-terminal amino acid sequence of TAP and 5′ rapid amplification of cDNA ends (RACE)-PCR. The cDNA encodes a mature protein of 449 amino acids with a 55-amino-acid signal peptide. The deduced amino acid sequence of TAP showed 56% identity with dye-decolorizing heme peroxidase (DYP) from the ascomycete Geotrichum candidum Dec 1, but no homology with other known peroxidases from fungi.

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Acknowledgements

This work was partially supported by grants for the Bioarchitect Research Program and the Eco Molecular Science Research Program from RIKEN. The authors are grateful to the Biomolecular Characterization Laboratory in RIKEN for amino acid sequence analysis, and C. Disyen and K. Sirihongsuwan for assistance.

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Authors and Affiliations

  1. International Cooperative Research Project, Japan Science and Technology Corporation (JST-ICORP), Kasetsart University Research and Development Institute, Bangkok 10900, Thailand

    T. Johjima, M. Ohkuma & T. Kudo

  2. Bioscience Technology Center, RIKEN (The Institute of Physical and Chemical Research), Saitama 351–0198, Wako, Japan

    T. Johjima, M. Ohkuma & T. Kudo

  3. Graduate School of Integrated Science, Yokohama City University, Yokohama 230–0045, Suehiro, Tsurumi-ku, Japan

    T. Kudo

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  1. T. Johjima
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  2. M. Ohkuma
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  3. T. Kudo
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Correspondence to T. Johjima.

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Johjima, T., Ohkuma, M. & Kudo, T. Isolation and cDNA cloning of novel hydrogen peroxide-dependent phenol oxidase from the basidiomycete Termitomyces albuminosus . Appl Microbiol Biotechnol 61, 220–225 (2003). https://doi.org/10.1007/s00253-003-1236-4

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  • DOI: https://doi.org/10.1007/s00253-003-1236-4

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